Effects of anticancer drugs on glia–glioma brain tumor model characterized by acoustic impedance microscopy

Soon, Thomas Tiong Kwong; Chean, Tan Wei; Yamada, Hikari; Takahashi, Kenta; Hozumi, Naohiro; Kobayashi, Kazuto

doi:10.7567/jjap.56.07jf15

Cited by 26 publications

(25 citation statements)

References 42 publications

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“…The speed of sound (SOS) through tissues [26][27][28][29][30][31][32][33][34][35] or acoustic impedance of samples [36,37] can be calculated by SAM, and two-dimensional distributions are mapped. Similarly, cells and organelles can be resolved by acoustic microscopy using higher frequencies of 100-1200 MHz [38][39][40][41][42][43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Designating Vulnerability of Atherosclerotic Plaques

Bilen¹

2020

Lipid Peroxidation Research

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Microcalcification is an indication of vulnerability of plaques in humans. With conventional imaging modalities, screening of micrometer-sized structures in vivo with high spatial resolution has not been achieved. The goal of this study is to evaluate the potentials of micro-computed tomography (micro-CT), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), time-resolved fluorescence spectroscopy (TRFS), scanning acoustic microscopy (SAM), and photoacoustic microscopy (PAM) in the determination of atherosclerotic plaques with microcalcifications and, therefore, the prospect of constructing a modality on a catheter system. The discrimination of microcalcifications within the fibrocalcific plaques and, therefore, the effectivity of these imaging techniques are discussed. The potential of quantum dots (QDs) in biological imaging is also elucidated since they attract great attention as contrast and therapeutic agents, owing unique properties including good light stability, low toxicity, strong fluorescence intensity, and changing emission wavelength with QD size, ranging from 10 to 100 Å in radius.

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Section: Introductionmentioning

confidence: 99%

Designating Vulnerability of Atherosclerotic Plaques

Bilen¹

2020

Lipid Peroxidation Research

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“…Two-dimensional maps of an area of around 5 × 5 mm are obtained in a couple of minutes. Similarly, cells and organelles can be examined using higher frequencies of 100 to 1200 MHz 21–28 .…”

Section: Introductionmentioning

confidence: 99%

Determination of Ultrastructural Properties of Human Carotid Atherosclerotic Plaques by Scanning Acoustic Microscopy, Micro-Computer Tomography, Scanning Electron Microscopy and Energy Dispersive X-Ray Spectroscopy

Bilen

Şener

Albeniz

et al. 2019

Sci Rep

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Microcalcification is the precursor of vulnerability of plaques in humans. Visualization of such small structures in vivo with high spatial resolution is an unsolved issue. The goal of this study is to evaluate the potential of scanning acoustic microscopy (SAM) in the determination of atherosclerotic plaques with calcifications by validating this technique with micro-computer tomography (micro-CT), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The fibrocalcific plaques were obtained from 12 different patients and initially examined with micro-CT. The images exhibited calcifications within these plaques. For imaging with SAM, approximately 5 μm thick slices were prepared. Sound speed values within calcified regions were measured to be greater than the ones in collagen-rich regions. These fibrocalcific plaques were also examined with SEM and EDS revealing collagen and calcium deposition within these samples. The consistency of the results obtained by all of the modalities involved in our study is an indication of the potential of SAM as a clinical tool for the diagnosis of vulnerable plaques.

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“…SAM calculates either the speed of sound (SOS) through tissues 12 – 21 or acoustic impedance of samples 22 , 23 and two-dimensional distributions are mapped. Similarly, acoustic microscopy can resolve cells and organelles using higher frequencies of 100 to 1200 MHz 24 – 31 .…”

Section: Introductionmentioning

confidence: 99%

Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques

Bilen

Gökbulut

Kafa

et al. 2018

Sci Rep

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Atherosclerotic plaques constitute the primary cause of heart attack and stroke. However, we still lack a clear identification of the plaques. Here, we evaluate the feasibility of scanning acoustic microscopy (SAM) and time-resolved fluorescence spectroscopy (TRFS) in atherosclerotic plaque characterization. We perform dual-modality microscopic imaging of the human carotid atherosclerotic plaques. We first show that the acoustic impedance values are statistically higher in calcified regions compared with the collagen-rich areas. We then use CdTe/CdS quantum dots for imaging the atherosclerotic plaques by TRFS and show that fluorescence lifetime values of the quantum dots in collagen-rich areas are notably different from the ones in calcified areas. In summary, both modalities are successful in differentiating the calcified regions from the collagen-rich areas within the plaques indicating that these techniques are confirmatory and may be combined to characterize atherosclerotic plaques in the future.

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Effects of anticancer drugs on glia–glioma brain tumor model characterized by acoustic impedance microscopy

Cited by 26 publications

References 42 publications

Designating Vulnerability of Atherosclerotic Plaques

Designating Vulnerability of Atherosclerotic Plaques

Determination of Ultrastructural Properties of Human Carotid Atherosclerotic Plaques by Scanning Acoustic Microscopy, Micro-Computer Tomography, Scanning Electron Microscopy and Energy Dispersive X-Ray Spectroscopy

Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques

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