Effects of antifreeze proteins on cryopreserved sterlet (Acipenser ruthenus) sperm motility variables and fertilization capacity

Xin, Miaomiao; Tučková, Vladimíra; Rodina, Marek; Kholodnyy, Vitaliy; Dadras, Hadiseh; Boryshpolets, Sergii; Shaliutina‐Kolešová, Anna; Linhart, Otomar

doi:10.1016/j.anireprosci.2018.07.007

Cited by 18 publications

(11 citation statements)

References 40 publications

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“…The present study revealed improved PMI values by 15% and 6% for sperm cryopreserve using 10 µg/mL AFPIII combined with 8% DMSO and 2% GLY, respectively, compared to PMI values reported by Kim et al [2]. It has been previously reported that the addition of AFPIII can robustly improve the PMI of cryopreserved sperm of sterlet [9] and seabream [27,28]. Results of the present study indicate that 10 µg/mL AFPIII has the potential to improve the plasma membrane stability of cryopreserved sperm of Pacific abalone.…”

Section: Discussionsupporting

confidence: 55%

“…Results of the present study indicate that 10 µg/mL AFPIII has the potential to improve the plasma membrane stability of cryopreserved sperm of Pacific abalone. The interaction between AFP and the sperm plasma membrane has been previously reported for different species [9,28].…”

Section: Discussionmentioning

confidence: 74%

“…Fertility is an important indicator of the reproduction success of cryopreserved sperm. The freeze-thaw process of cryopreservation is recognized as a detrimental factor for sperm function and fertility [9]. The goal of the improvement in quality of cryopreserved sperm is to achieve a higher fertilization rate.…”

Section: Discussionmentioning

confidence: 99%

“…Although penetrating cryoprotectants can increase cell membrane fluidity through protein and lipid reorganization [5], they can also hamper intracellular ice crystal formation by decreasing the freezing point temperature, which is known to be an undesirable mechanism of sperm death [6,7]. The sperm cryopreservation process involves changes of extreme temperature that can lead to cryoinjuries (decreases of plasma membrane integrity, acrosomal integrity, mitochondrial membrane potential, motility, and viability) of morphological structures and physiology [8][9][10]. These cryoinjuries can alter the functional status of sperm proteins in the plasma membrane, midpiece, nucleus, and flagella, thus affecting the motility and fertilization capacity of sperm [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Considering the above facts, the sperm cryopreservation protocol for this commercially valuable species needs to be improved. Sperm cryopreservation using antifreeze proteins (AFPs) can improve post-thaw sperm quality of different species of fish, including sea bream [27][28][29], sterlet [9,15], common carp [30], and Persian sturgeon [31]. AFPs are specific proteins that can protect cells by reducing the freezing point, interacting with the plasma membrane, adjusting ice crystallization, and protecting recrystallization [9,[32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Antifreeze Protein III on Sperm Cryopreservation of Pacific Abalone, Haliotis discus hannai

Hossen

Sharker

Cho

et al. 2021

IJMS

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Pacific abalone (Haliotis discus hannai) is a highly commercial seafood in Southeast Asia. The aim of the present study was to improve the sperm cryopreservation technique for this valuable species using an antifreeze protein III (AFPIII). Post-thaw sperm quality parameters including motility, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), DNA integrity, fertility, hatchability, and mRNA abundance level of heat shock protein 90 (HSP90) were determined to ensure improvement of the cryopreservation technique. Post-thaw motility of sperm cryopreserved with AFPIII at 10 µg/mL combined with 8% dimethyl sulfoxide (DMSO) (61.3 ± 2.7%), 8% ethylene glycol (EG) (54.3 ± 3.3%), 6% propylene glycol (PG) (36.6 ± 2.6%), or 2% glycerol (GLY) (51.7 ± 3.0%) was significantly improved than that of sperm cryopreserved without AFPIII. Post-thaw motility of sperm cryopreserved with 2% MeOH and 1 µg/mL of AFPIII was also improved than that of sperm cryopreserved without AFPIII. A combination of 10 µg/mL AFPIII with 8% DMSO resulted in the highest post-thaw motility, showing AI of 60.1 ± 3.9%, PMI of 67.2 ± 4.0%, and MMP of 59.1 ± 4.3%. DNA integrity of sperm cryopreserved using 10 µg/mL AFPIII combined with 8% DMSO was not significantly (p > 0.05) different from that of fresh sperm. Cryopreservation using a combination of AFPIII with 8% DMSO improved fertilization and hatching rates of sperm compared to that of cryopreservation without supplementation of 10 µg/mL AFPIII. Sperm cryopreserved using AFPIII showed higher mRNA abundance levels of HSP90 than those cryopreserved without AFPIII. Results of the present study suggest that 10 µg/mL AFPIII combined with 8% DMSO can be used for large scale cryopreservation of Pacific abalone sperm and for hatchery production.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Antifreeze Protein III on Sperm Cryopreservation of Pacific Abalone, Haliotis discus hannai

Hossen

Sharker

Cho

et al. 2021

IJMS

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Effect of appropriate extenders to maintain sperm functionality during short-term storage of sterlet (Acipenser ruthenus) sperm with fertilization assay under hatchery conditions

Shazada,

Siddique,

Zhang

et al. 2024

Fish Physiol Biochem

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Short-term storage and management of sperm in vitro is an easy and economical process in which suitable extenders can be utilized to extend the storage period and prevent sperm function impairment. Therefore, the current study aimed to evaluate the effect of suitable extenders during the short-term storage of sterlet sperm and determine their fertilizing capacity and hatching success. Three extenders containing a composition of 16, 20, and 24 mM NaCl, 1 mM KCl, 0.1 mM CaCl2, 10 mM Tris, pH 8.0 with osmolarity of 46, 55, and 62 mOsm/kg, were used to dilute the sperm of four sexually mature sterlet males (n = 4). Using a CASA system, the motility and velocity of undiluted and diluted sperm with extenders (E1 – E3) were assessed over 6 days at 0–2 °C. The short-term stored diluted sperm was then used in the fertilization and hatching assay, and undiluted fresh and stored sperm was used as a control. A two-way factorial analysis of variance (ANOVA) model confirmed significant effects on sperm motility, curvilinear velocity (VCL), and straight-line velocity (VSL) (P < 0.001), as well as their interaction with the extender. The model was decomposed into a one-way ANOVA to examine the impacts of extenders and storage time. With increasing storage periods, the sperm motility and velocity gradually decreased for diluted sperm with three extenders (E1–E3) but sharply decreased for undiluted sperm (Control). The motility of undiluted sperm was found 3.77 ± 4.09% at 4 days, whereas sperm diluted with extenders showed 57.57 ± 12.33% (E1), 64.34 ± 11.86% (E2), and 61.40 ± 12.41% (E3) motility at 6 days. This study explored extenders optimized with higher osmolarity (39–62 mOsm/kg) and lower K+ (1 mmol/L) as the most suitable medium for storing sterlet sperm for 6 days. After 6 days post storage, sperm diluted with extenders E1–E3 achieved a fertilization rate of 31.29 ± 14.2%, 31.66 ± 8.84%, and 30.67 ± 10.02%, respectively, and hatching success of 29.58 ± 13.4%, 30.50 ± 7.89%, and 27.95 ± 9.62%, respectively with freshly ovulated eggs.

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Seminal plasma transferrin effects on cryopreserved common carp Cyprinus carpio sperm and comparison with bovine serum albumin and antifreeze proteins

Shaliutina‐Kolešová

Dietrich

Xian

et al. 2019

Animal Reproduction Science

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Effects of antifreeze proteins on cryopreserved sterlet (Acipenser ruthenus) sperm motility variables and fertilization capacity

Cited by 18 publications

References 40 publications

Effects of Antifreeze Protein III on Sperm Cryopreservation of Pacific Abalone, Haliotis discus hannai

Effects of Antifreeze Protein III on Sperm Cryopreservation of Pacific Abalone, Haliotis discus hannai

Effect of appropriate extenders to maintain sperm functionality during short-term storage of sterlet (Acipenser ruthenus) sperm with fertilization assay under hatchery conditions

Seminal plasma transferrin effects on cryopreserved common carp Cyprinus carpio sperm and comparison with bovine serum albumin and antifreeze proteins

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