Effects of antiinflammatory drugs on arthritic cartilage: A high‐frequency quantitative ultrasound study in rats

Jaffre, B.; Watrin, A.; Lœuille, Damien; Gillet, Pierre; Netter, Patrick; Laugier, Pascal; Saied, A.

doi:10.1002/art.11023

Cited by 27 publications

(14 citation statements)

References 28 publications

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“…Cherin et al (20) demonstrated a relationship between quantitative ultrasound and maturation-related changes in rat cartilage, while Jaffre et al (21) reported that quantitative 55-MHz ultrasound allowed detection of early cartilage lesions due to experimental arthritis, as well as enabling monitoring of the effects of antiinflammatory drugs. Therefore, high-frequency ultrasonography could be useful for investigating structural changes in the cartilage matrix and evaluating the efficacy of specific therapeutic agents.…”

Section: Discussionmentioning

confidence: 99%

Differential acoustic properties of early cartilage lesions in living human knee and ankle joints

Hattori¹,

Takakura²,

Ishimura³

et al. 2005

Arthritis & Rheumatism

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Objective. Although numerous studies have been performed to determine whether there are histologic, biochemical, biomechanical, and metabolic differences between knee and ankle cartilage, none have investigated the presence of such differences in living human cartilage. We previously developed an ultrasonic evaluation system for articular cartilage that analyzes the A-mode echogram using wavelet transformation. The current study was undertaken to determine whether the acoustic properties of living human cartilage differ between knee and ankle joints.Methods. Twenty-eight patients were subjected to ultrasonic evaluation under arthroscopy. After arthroscopic grading, the cartilage was measured using an ultrasonic probe. Two quantitative parameters were used, i.e., the maximum magnitude and the echo duration at the 95% interval of the maximum magnitude.Results. In intact cartilage, the maximum magnitude and echo duration did not differ between the knee and the ankle. In lesional cartilage, in contrast, the maximum magnitude was higher, and the echo duration was shorter, in the ankle than in the knee. These differences were statistically significant.Conclusion. Ultrasound findings could be used to judge the degree of early cartilage degeneration in vivo on the basis of objective data such as the maximum magnitude and echo duration. Because we were unable to quantitatively analyze the biochemical and biomechanical properties of the cartilage in this study, our biochemical and biomechanical findings are based only on qualitative assessment. Nevertheless, the results indicate that this ultrasonic evaluation system may be useful for elucidating the processes of articular cartilage degeneration in osteoarthritis.Osteoarthritis (OA), also referred to as degenerative joint disease, is one of the most prevalent chronic conditions, although not all joints are equally affected by the disease (1). Studies of cadavers have revealed that full-thickness cartilage defects occur ϳ20 times more often in the knee than in the ankle (2,3). Symptomatic OA with radiographic changes occurs ϳ8-9.4 times more frequently in the knee than in the ankle in patients seeking treatment (4,5). Therefore, several researchers have focused on comparing the cartilages from these 2 joints to determine whether they possess histologic, biochemical, biomechanical, and/or metabolic differences (6-10). There has been a great deal of effort, in studies using cartilage from cadavers, to elucidate the differences between these 2 joints. Indeed, such studies may help us to understand the different susceptibilities of these 2 joints and the pathogenesis of OA in general. However, none of these investigations have been conducted with living human cartilage.We previously developed a novel system for evaluating articular cartilage, in which the acoustic properties of articular cartilage are measured by introducing an ultrasound probe into the knee joint under arthroscopy (11,12). The analysis system is based on wavelet transformation of the reflex echogram from arti...

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Section: Discussionmentioning

confidence: 99%

Differential acoustic properties of early cartilage lesions in living human knee and ankle joints

Hattori¹,

Takakura²,

Ishimura³

et al. 2005

Arthritis & Rheumatism

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“…Results of these studies are variable. For example, treatment with aspirin, diclofenac, ibuprofen, indomethacin, and tenoxicam, was associated with impaired bone healing in rats and rabbits [69][70][71][72][73][74][75][76][77][78][79][80][81][82][83][84][85][86][87]. The main effects observed in those models included inhibition of Haversian (cortical bone) remodeling, alterations in spinal fusions, poor mineralization of bone matrix, decreased osteoid formation and callus bone formation, decreased bone mineral content, reduced mechanical strength of replacement tissue, decreased amounts of cortical bone and inhibition of new bone formation [73,77,[80][81][82][88][89][90][91][92].…”

Section: Effects Of Non-selective Nsaids On Bone Healingmentioning

confidence: 99%

Effects of cyclooxygenase inhibition on bone, tendon, and ligament healing

Radi

Khan

2005

Inflamm. res.

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Cyclooxygenases (COX-1 and COX-2) catalyze the conversion of arachidonic acid to prostaglandins (PGs). PGs play a significant role in bone metabolism in health and disease. Conventional non-selective, non-steroidal anti-inflammatory drugs (NSAIDs) and selective COX-2 inhibitors (COXIBs) are widely used in patients with musculoskeletal conditions and as a post-surgical analgesics. Due to their effects on PG synthesis, these drugs have been hypothesized to affect the healing process of bone fractures and orthopedic surgical sites. A variety of experimental models of bone, ligament, and tendon repair have assessed the effects of these selective and non-selective COX inhibitors in animals, but with variable outcomes. At this time, large-scale, robust clinical study data do not exist, limiting the relevant assessment of experimental animal data to humans. Here, we provide a critical review of available data on the role of PGs and the effect of COX inhibitors on bone, tendon, and ligament repair. Collectively, this assessment suggests potential involvement of PGs in the healing process of these tissues via modulation by non-selective NSAIDs and selective COX-2 inhibitors.

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“…All previous studies using the same model have described findings more than 10 days after induction. mortem analysis, examined the disease at more advanced stages, restricted imaging to a single modality (either MRI or CT), with or without direct histological correlation (1,7,(12)(13)(14). In our study, high-resolution microMRI and microCT successfully detected very early inflammatory changes within the first week of injection of the adjuvant.…”

Section: Discussionmentioning

confidence: 87%

Early findings of small‐animal MRI and small‐animal computed tomography correlate with histological changes in a rat model of rheumatoid arthritis

Lee

Greve

Leaffer³

et al. 2008

NMR in Biomedicine

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With the use of a commonly utilized animal model of rheumatoid arthritis, the central goal of this work was to determine how well the small-animal imaging tools, small-animal MRI (microMRI) and small-animal X-ray computed tomography (microCT), can detect very early histological changes that occur immediately after induction of the disease. Arthritis was induced in rats by injecting complete Freund's adjuvant into the tail. Right hind paws of living rats were evaluated with 4.7 T microMRI with T1-weighted spin echo and inversion recovery sequences. Paw specimens were also evaluated with microCT and by histological examination (n = 29). MicroMR images were scored for the presence of joint effusion, soft tissue swelling, bone marrow changes, and bone erosions. MicroCT measured bone mineral density (BMD). Histology scores were obtained from representative slides from the same rats. The correlation between BMD, MRI and histology was analyzed using linear regression analysis and analysis of covariance. MRI abnormalities were detected on day 5 after induction as joint effusion and soft tissue swelling, followed by bone marrow changes on day 6 and bone erosion on day 8. BMD measured by microCT decreased, the decrease becoming significant on day 7 (P < 0.019). Soft tissue swelling, joint effusion, and bone erosion scores on microMRI correlated with histology (r2 approximately 0.7). Bone marrow changes were seen more clearly with microMRI than by histological examination. Bone loss could be detected earlier by microCT than on histological sections. In conclusion, microMRI and microCT can be used to evaluate early disease changes within 1 week of induction in the adjuvant-induced arthritis model, and have the ability to detect certain manifestations of disease earlier than histological analysis. The use of small-animal imaging techniques potentially allows earlier diagnosis, improved subject stratification, earlier drug implementation, and therefore improved drug trials in animal models of rheumatoid arthritis.

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Effects of antiinflammatory drugs on arthritic cartilage: A high‐frequency quantitative ultrasound study in rats

Cited by 27 publications

References 28 publications

Differential acoustic properties of early cartilage lesions in living human knee and ankle joints

Differential acoustic properties of early cartilage lesions in living human knee and ankle joints

Effects of cyclooxygenase inhibition on bone, tendon, and ligament healing

Early findings of small‐animal MRI and small‐animal computed tomography correlate with histological changes in a rat model of rheumatoid arthritis

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