Effects of Antimicrobial Peptides Surfactin Administration on Growth Performance, Intestinal Digestive Enzymes Activities and Some Serum Biochemical Parameters of Orange-spotted Grouper (Epinephelus coioides) JuvenilesShao

Abstract

This trial was conducted to investigate the effects of dietary surfactin supplementation on the growth performance, intestinal digestive enzyme activities, and some serum biochemical parameters of orange-spotted grouper (Epinephelus coioides) juveniles. Three hundred and sixty fish were randomly divided into six treatment groups with four replicates in each group and 15 fish in each replicate. The dietary surfactin levels of four treatment groups were 0 (control group), 25, 50, 100, 150, and 200 mg/kg, respectively. The trial period was 8 weeks. Compared with the control group, final body weight, weight gain rate, and feed conversion ratio were improved significantly by 100mg/kg surfactin supplementation (P<0.05). No significant differences of feeding rate and survival rate were found between the control group and all surfactin supplementation groups (P>0.05). Protease and lipase activity in intestine of 50, 100, and 150 mg/kg surfactin supplementation groups were significantly higher than those of the control group (P<0.05). Amylase activity was not affected significantly by surfactin supplementation (P>0.05). The blood urea nitrogen levels, and acid phosphatase and alkaline phosphatase activity in 100, 150, and 200 mg/kg surfactin supplementation groups were significantly ameliorated (P<0.05). The albumin level of 100 mg/kg surfactin supplementation group was significantly higher than that of the control group. Lysozyme activity of 50, 100, and 150 mg/kg surfactin supplementation groups was significantly higher than the control group (P<0.05). In conclusion, we demonstrated a promotion of growth performance and improvement of intestinal digestive enzyme activity, and some serum biochemical parameters of the orange-spotted grouper juveniles by appropriate surfactin levels supplemented in diet.

Effects of antimicrobial peptide APSH‐07 on the growth performance, anti‐oxidation responses, stress resistance and intestine microbiota in large yellow croakerLarimichthys crocea

“…Kumari et al () found that dietary lactoferrin at 100 mg/kg level significantly enhanced serum LZM activity in Asian catfish Clarias batrachus ( p < .05). In the research of Zhai et al (), the higher activity of LZM in the 100 mg/kg of dietary surfactin‐supplemented groups indicates that the orange‐spotted grouper fed with surfactin‐supplemented diets might have strong innate immune capability. Similarly, a clear incremental trend was observed in activity of AKP and ACP in dietary antimicrobial peptide APSH‐07‐supplemented groups.…”

“…It is capable of promoting phagocytosis through modification of the pathogen surface molecules (Yin et al, ). Due to the stimulation of antimicrobial peptide APSH‐07, AKP could involve in the cell metabolism of fish, improving the non‐specific immunity (Zhai et al, ). The above results in present study suggested that the antimicrobial peptide APSH‐07 could play an active role in the activation of non‐specific defence and anti‐oxidation in large yellow croaker.…”

“…In the research of Zhou et al (), the supply of apidaecin (15 and 30 mg/kg) could improve the growth and feed utilization of common carp Cyprinus carpio . Zhai, Sun, and Chen () revealed that growth performance of orange‐spotted grouper was significantly improved by 100 mg/kg of dietary surfactin supplementation. In the study of Lygren et al (), SGR was unaffected by the dietary bovine lactoferrin in Atlantic salmon.…”

Influences of dietary antimicrobial peptide APSH‐07 on the growth performance, immune response and vibriosis resistance of abaloneHaliotis discus hannaiIno

“…The activity of LZM in muscle (Chen et al., 2010), haemolymph and hepatopancreas (Song et al., 2010) of L. vannamei was previously reported to be increased after feeding with the dietary antimicrobial peptide (extractives from houseflies, Musca domestica and Penaeidins respectively). Meanwhile, the promoting effect of antimicrobial peptides on the activity of LZM in serum has been widely demonstrated in fish, such as Asian catfish Clarias batrachu (Kumari, Swain, & Sahoo, 2003), orange‐spotted grouper E. coioides (Zhai, Sun, & Chen, 2017) and large yellow croaker L. crocea (Ge et al., 2019). Increased activities of ACP in serum were observed in common carp C. carpio (Nguyen et al., 2016) and large yellow croaker L. crocea (Ge et al., 2019) fed with dietary antimicrobial peptides.…”

Dietary emulsifier and antioxidant improved astaxanthin utilization and antioxidant capacity of rainbow trout ( Oncorhynchus mykiss )

“…Note: Different lowercase letters of values at the same sampling time indicate significant differences among groups (p < .05). AST-50, 50 mg kg −1 of synthetic astaxanthin; AST-100, 100 mg kg −1 of synthetic astaxanthin; CON, control; ST, sodium taurocholate; SUR, surfactin; TP, tea polyphenols of tilapia (Oreochromis niloticus) (Zhai et al, 2016a) and orangespotted grouper (Epinephelus coioides) (Zhai et al, 2017). As emulsifiers, sodium taurocholate and surfactant can increase the contacting area between lipid and lipase through emulsification and improve the intestinal digestive enzymes activities, thus promoting the digestion and absorption of lipid, then the growth of farmed animals.…”