Effects of Antiseptics on Pulpal Healing under Calcium Hydroxide Pulp Capping: A Pilot Study

Abstract

Objectives:The objective of this pilot study was to evaluate the effects of three different antiseptic materials on healing processes of direct pulp therapies with Ca(OH)2 histopathologically.Methods:Twenty-eight upper and lower first molar teeth from 7 male Wistar rats were used in this study. Four cavities were prepared in each rat in four quadrants, and each quadrant represented different experimental groups. In Group I: 0.5% sodium hypochlorite (NaOCl); in Group II: 2% chlorhexidine digluconate (CHX); in Group III: 0.1% octenidine dihydrochloride (OCT); and in Group IV 0.9% sterile saline was applied to the exposure site with a sterile cotton pellet for 3 minutes. After hemorrhage control, the pulps were capped with hard setting Ca(OH)2 and, finally, restored with IRM. The animals were euthanized at 21 days post-operatively. After sacrificing, routine histological procedures were performed and evaluated statistically with non-parametric Kruskal-Wallis test among the groups and two-by-two comparisons by using the Mann-Whitney U test for inflammatory response and tissue organization scores at the confidence interval of 95%.Results:There were significant differences in inflammatory response and tissue organization scores between the groups (P<.05). Statistical evaluation of inflammatory response showed that Group IV was significantly different from Groups I, II and III separately with a higher inflammatory cell response (P<.05) whereas no significant differences were detected between the other groups in two-by-two comparisons (P>.05). Healthy coronal and radicular pulp tissue organization scores indicated that the Group I has better pulp tissue organization than Group IV and this was significantly different (P<.05) whereas no significant differences were observed between the other groups separately (P>.05).Conclusions:The antiseptic materials used in this study created an environment that, rather than saline solution, may affect clinical and histological success in a positive way.

Chlorhexidine, ethanol, lipopolysaccharide and nicotine do not enhance the cytotoxicity of a calcium hydroxide pulp capping material

“…Studies by other authors show that chlorhexidine does not negatively interfere with the properties of calcium hydroxide (Signoretti et al. 2011, Bal et al. 2011).…”

Direct Pulp Capping: Which is the Most Effective Biomaterial? A Retrospective Clinical Study

“…Bleeding should be controlled by exerting pressure at the exposure site for 3 to 5 min with a sterile cotton ball soaked in the haemostatic/disinfection solution. Several authors have studied the efficacy and influence of these solutions on pulp tissue regeneration [34,35,36,37]. Although saline has limited effects, it is traditionally the most widely used.…”

Direct pulp capping of primary molars using a novel fast-setting calcium silicate cement: a randomized clinical trial with 12-month follow-up

“…In the current study, peripheral infected dentin was removed thoroughly and the case selection was strictly limited to exposure site less than one mm with controllable hemostasis. In an attempt to reduce the bacterial load, we constantly irrigated the cavity with sodium hypochlorite before the pulpal exposure and chlorhexidine after the pulpal exposure [40].…”