Effects of arabinoxylan and chlorogenic acid on the intestinal microbiota in dextran sulfate sodium–treated mice

Abstract

Dietary non-starch polysaccharides and phenolics are usually ingested at the same time. They are both regarded as prebiotics, and they regulate the intestinal microbiota through various mechanisms. Notably, however, reports of their combined or synergistic effects are rare. Arabinoxylan (AX), a polysaccharide, and chlorogenic acid (CA), a polyphenol, are widely consumed, and their effects on the microbiota have previously been discussed. In the present study, they were given to dextran sulfate sodium (DSS)–treated mice, separately and together, and the intestinal microbiota were investigated by high-throughput sequencing. The data showed that CA attenuated body weight loss, colon shortening, and histological damage in DSS-treated mice, while neither AX nor the AX+CA combination exhibited any ameliorating potential. AX+CA had less of a modulating effect on intestinal microbiota profiles than did CA. AX+CA administration increased the relative abundance of Flavonifractor, Coprobacillus, and Clostridium_XlVa, and decreased the abundance of Robinsoniella and Lactobacillus. Compared to AX and CA, AX+CA contributed to a more complicated shift in the biological functions of the intestinal microbiotaAX seemed to weaken the beneficial effects of CA, at least in the present experimental model of DSS-induced colitis. The combined effects and mechanisms of dietary polysaccharides and phenolic compounds on the intestinal microbiota and on overall health still need to be further investigated.

Cucurbitacin E Alleviates Colonic Barrier Function Impairment and Inflammation Response and Improves Microbial Composition on Experimental Colitis Models

“…However, these changes varied in different experiments. 12,20,21 These inconsistent results indicate that the effects of DSS on gut microbes may also affect by host microbial composition and other factors. Nevertheless, cucurbitacin E supplementation alleviated the alterations caused by the DSS treatment.…”

Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells

“…In contrast, a previous study failed to determine a preventive effect against DSS-induced acute colitis, which is triggered by gut barrier destruction and subsequent innate immune cell activation [ 22 ]. The reason that AX did not affect DSS-induced colitis might be due to the low dosage of AX used in the previous report.…”

“…However, few studies have demonstrated the beneficial effects of AX in IBD. Only one recent study has evaluated the impact of AX intervention on DSS-induced colitis, an IBD model triggered by an intestinal barrier disorder, followed by bacterial invasion and subsequent innate immune cell activation [ 22 ]. However, no conclusions have been drawn regarding the preventive effects of AX against colitis.…”

Modified rice bran arabinoxylan as a nutraceutical in health and disease—A scoping review with bibliometric analysis

“…For example, RBAC research has been featured in research characterising as a functional ingredient of defatted rice bran [ 58 ] and a fermented rice bran by-product with anticancer properties [ 22 , 59 – 61 ], as a polyphenolic compound based on hemicelluloses [ 62 ] or polysaccharides [ 63 ] beneficial for gastrointestinal disorders and cancer [ 64 ], and as natural food to protect against gamma-induced oxidative damage [ 65 ]. RBAC is also commonly cited as a representative arabinoxylan in the diverse group of dietary fibres that exist in food [ 66 ] with anticancer and antioxidant effects [ 67 ] and the capacity to modulate gut microbiota [ 68 ] for combating human chronic diseases [ 69 ]. Results from RBAC research also affected the research design of oncological experimental models [ 70 ], especially in preventing tumour growth [ 71 ].…”