Effects of arachidonate on cultured pig thyroid cells and their stimulation by thyrotropin

Gérard, Céline; Haye, Bernard

doi:10.1016/0014-5793(81)80419-x

Cited by 11 publications

(3 citation statements)

References 13 publications

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“…A previous analysis has shown variations of annexin content under activation of the adenylate cyclase system by TSH [16]. TSH introduction to thyroid cells in vitro induces thyroid hormone biosynthesis (shown by an increase in protein-binding iodine) [24]. This hormonal neosynthesis does not lead to a decrease in the annexin content as expected from in vivo experiments.…”

Section: Discussionmentioning

confidence: 70%

In vivo Modulation of Annexins I, II and V Expression by Thyroxine and Methylthiouracil

Btaouri

Claisse

Bellon

et al. 1996

European Journal of Biochemistry

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Regulation of annexin concentration and localization were investigated in thyroid tissues of hypothyroid [methylthiouracil (MeSur) treatment], euthyroid (control) and hyperthyroid [thyroxine (T4) treatment] rats.A low level of circulating thyroid hormones induces a decrease of total thyroid calcium-binding protein concentration when compared with the concentration in unstimulated animals. Conversely, concentrations of annexins I, I1 and V increase. The accumulation of these proteins in two subcellular compartments (cytosolic and particulate fractions) can be reversed by addition of thyroid hormones. The finding of a specific increase in annexins concentration in thyroid-hormone-deficient rats, with a general decrease of the total calcium-binding protein content points to a very important role of these proteins in the cells. Furthermore, hyperthyroidism gives opposite results. To investigate the transduction pathway of annexins I-, 11-and V-induced biosynthesis by thyroid hormones in thyroid glands, we used cultured pig thyroid cells as in vitro model system. In previous work [16], we have shown that annexin concentrations and localization are under TSH control via the adenylate cyclase pathway. In the presence of MeSur (in the culture medium), the protein-binding iodine remains low, indicative of weak thyroid hormone synthesis (data not shown) and that the annexins content is unchanged.These results suggest that, in thyroid tissue, an indirect mechanism links thyroid hormones to annexin expressions via the TSH feed-back loop, and excludes autocrine regulation.

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Section: Discussionmentioning

confidence: 70%

In vivo Modulation of Annexins I, II and V Expression by Thyroxine and Methylthiouracil

Btaouri

Claisse

Bellon

et al. 1996

European Journal of Biochemistry

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“…The preparation, the culture and washing of thyroid cells were as in [1,2]. The 'chronic treatment' was applied during the culture period of the cells.…”

Section: Preparation Culture Washings Of Thyroid Cellsmentioning

confidence: 99%

“…In thyroid, the arachidonate liberated by the TSH action on specific PI phospholipases [3-61, which is a substrate for cycle-oxygenase and lipoxygenase [3,7-91, modulates phospholipid metabolism [ 1] and the iodination process [ 1, lo].…”

Section: Cyclic Amp Assaymentioning

confidence: 99%

Effects of eicosatetraynoic acid (ETYA) on cultured pig thyroid cells

Haye¹,

Gérard²

1982

FEBS Letters

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The arachidonate inhibition of the adenylate-cyclase system of cultured pig thyroid cells was not mediated by cyclooxygenase, lipoxygenase or peroxidase metabolites. Indeed ETYA, an inhibitor of cyclooxygenase and lipoxygenase, and methimazole, an inhibitor of peroxidase and iodination were without effect on the arachidonate inhibition. Moreover the effect of arachidonate was amplified by a combination with ETYA. In 32P incorporation experiments we observed a modification of the labelling of individual phospholipids of cultured pig thyroid cells resulting in a decrease into phosphatidylinositol (PI) and an increase into phosphatidate (PA) of arachidonate and ETYA-treated cells. These results may be explained by an inhibition of CDP-diacylglycerol : inositol transferase and conversely a stimulation of PI specific phospholipase C yielding a decrease in PI and an increase in PA, which inhibits in turn adenylate cyclase activity possibly by Ca*+ translocation. Eicosatetraynoic acid Arachidonic acid Porcine thyroid cell PhospholipidThyrotropin Cyclic AMP

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Control by TSH of the Production by Thyroid Cells of an Inositol Phosphateglycan, Putative Mediator of the cAMP-Independent Effects in the Gland

Martiny

Thuilliez

Lambert

et al. 1990

Biology of Cellular Transducing Signals

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Effects of arachidonate on cultured pig thyroid cells and their stimulation by thyrotropin

Cited by 11 publications

References 13 publications

In vivo Modulation of Annexins I, II and V Expression by Thyroxine and Methylthiouracil

In vivo Modulation of Annexins I, II and V Expression by Thyroxine and Methylthiouracil

Effects of eicosatetraynoic acid (ETYA) on cultured pig thyroid cells

Control by TSH of the Production by Thyroid Cells of an Inositol Phosphateglycan, Putative Mediator of the cAMP-Independent Effects in the Gland

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