Effects of Arbuscular mycorrhizal fungi isolated from white clovers (Trifolium repensL) on soil bacteria and fungi

Abstract

White clover potted experiments were performed to investigate the effects of seven indigenous arbuscular mycorrhizal fungi (AMF) communities isolated from different test plots subjected to long-term fertilisation on soil enzyme activities, number of soil bacteria and fungi. The results showed that the inoculation of arbuscular mycorrhizal fungi communities increased the mycorrhizal infection rate of the plants and promoted the growth of plants. The Mnp treatment was most effective. The shoot biomass, root biomass, potassium and nitrogen uptake of the white clover in Mnp treatment group were increased by 61.54%, 84.00%, 62.50% and 46.71% respectively, compared with those in non-inoculation treatment. The inoculation of AMF communities had little effect on the number of bacteria in the soil, but significantly increased the number of soil fungi. Mnk treatment group had the highest number of fungi in the soil, which was 9.91 times that of the non-inoculation treatment group. The catalase and dehydrogenase activities were both significantly improved in Mnp treatment by 28.12% and 205.38% respectively, compared with those of the control treatment (-M). The urease, invertase and cellulase activities reached the highest levels in the Mck treatment; they were increased by 142.79%, 41.17% and 77.62% respectively, compared with those of the control treatment. Pearson correlation analysis showed that the soil enzyme activity was not correlated with the mycorrhizal infection rate, but correlated with the spore number of the AMF community. The impact of AMF community on soil quality is important for us to understand the function of the ecosystems. Relevant study provides important guidance for maintaining the balance of the soil-plant system and the development of sustainable agriculture.

Response of a wild-type and modern cowpea cultivars to arbuscular mycorrhizal inoculation in sterilized and non-sterilized soil

“…The study demonstrated that AMF inoculation increased nodulation and N fixation, which in turn improved N nutrition. Similarly, a study done by Huang et al (2014), on white clover (Trifolium repens) found a significant correlation between percentage AMF colonization and number of nodules. Nonetheless, contrary findings were obtained in alfalfa (Catford et al 2003), whereby colonization of roots with AMF systematically inhibited further mycorrhization as well as nodule formation.…”

Illumina MiSeq Sequencing Reveals Correlations among Fruit Ingredients, Environmental Factors, and AMF Communities in Three Lycium Barbarum Producing Regions of China

“…Spearman correlation analysis revealed that three genera, Claroideoglomus , Dominikia , and Funneliformis , were significantly negatively correlated with urease, showing a negative effect of urease and total nitrogen. AMF inoculation is known to increase the soil urease, convertase, and cellulase activities ( 23 ). Ammonia, an enzymatic product of urease, is not irreplaceable.…”

“…The difference in quantity and diversity at the genus level of the AMF communities leads to different amounts of root exudates. These differences lead to different soil invertase activity and cellulase activity ( 23 ). Besides, soil dehydrogenases are closely related to soil respiratory metabolism, citric acid cycle, and nitrogen metabolism and can be used to represent the metabolic activities of soil microorganisms.…”

Arbuscular Mycorrhizal Fungi Enhance Sea Buckthorn Growth in Coal Mining Subsidence Areas in Northwest China

“…showed that inoculation with mycorrhizal fungi significantly increased the number of fungi in soil in which white clover was growing but had little effect on the number of bacteria in the soil, while Li et al . found that AMF increased the numbers of bacteria and actinomycetes and reduced the number of fungi [ 41 , 42 ]. Here, AMF improved the rhizosphere microenvironment, nutrient status and organic matter content, together with the acid phosphatase activity, which may have contributed to the increase in the number of microorganisms and in the metabolic activity of the rhizosphere.…”