2009
DOI: 10.1016/j.bej.2008.12.017
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Effects of arcA and arcB genes knockout on the metabolism in Escherichia coli under aerobic condition

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Cited by 45 publications
(34 citation statements)
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“…A summary of genes whose knockout mutant fluxes have been reported in literature are shown in Figure 1 [211,2643]. While knockout flux studies are clearly an area of strong interest, bias in coverage and poor comparability of results have prevented these results from being utilized to their full potential.…”
Section: Metabolic Flux Analysis Studies Of E Coli Knockoutsmentioning
confidence: 99%
“…A summary of genes whose knockout mutant fluxes have been reported in literature are shown in Figure 1 [211,2643]. While knockout flux studies are clearly an area of strong interest, bias in coverage and poor comparability of results have prevented these results from being utilized to their full potential.…”
Section: Metabolic Flux Analysis Studies Of E Coli Knockoutsmentioning
confidence: 99%
“…135 genes (54), mainly acting as a negative transcriptional regulator of genes encoding enzymes involved in aerobic pathways, such as the major dehydrogenase enzymes of the tricarboxylic acid (TCA) cycle and the glyoxylate shunt (60). Genes encoding enzymes related to fermentation pathways become activated by ArcAϳP under microoxic or anoxic conditions (36,44,50). Respiratory pathways are also affected by the ArcBA system, and the expression of cyoABCDE and cydAB is repressed and activated by ArcAϳP, respectively (36,60).…”
mentioning
confidence: 99%
“…ArcA (anaerobic redox control) is the cytosolic response regulator of the dual-component regulator system ArcAB [31, 32]. It is reported that phosphorylated ArcA can repress genes expression of TCA cycle and glyoxylate shunt, such as gltA , acnAB , icdA , sucABCD , sdhCDAB , fumA , mdh, and aceB [33–35].…”
Section: Discussionmentioning
confidence: 99%
“…Our previous results demonstrated that the acetate concentrations of arcA mutants in BL21(DE3) were decreased by more than 70% and the productions of PG and 3HP were increased by more than twice. The decreased acetate concentrations and increased PG and 3HP productions of arcA mutants could be caused by the following reasons: the higher activity of TCA cycle and glyoxylate pathway; the more metabolic flux through pyruvate dehydrogenase (PDH) complex, which catalyzes pyruvate to acetyl-CoA [13, 32, 36]. IclR (isocitrate lyase regulator) is a local regulator, inhibiting genes expression of aceBAK operon in glyoxylate pathway.…”
Section: Discussionmentioning
confidence: 99%