Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes—A possible role of betel-quid chewing in metabolic syndrome

Hsu, Hsin-Fen; Tsou, Tsui‐Chun; Chao, How‐Ran; Shy, Cherng-Gueih; Kuo, Ya‐Ting; Tsai, Feng-Yuan; Yeh, Szu-Ching; Ko, Ying‐Chin

doi:10.1016/j.taap.2010.04.008

Cited by 61 publications

(48 citation statements)

References 47 publications

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“…Another research by Hsu et al (2010) demonstrated that arecoline could inhibit adipogenic differentiation, and induced adenylyl cyclase-dependent lipolysis, interfered with insulin-induced glucose uptake. In addition, a research reported the effects of arecoline on pancreas-duodenum homeobox-1 (PDX-1) mRNA expression in rats with type 2 diabetes mellitus, and found that arecoline could prevent the dysfunction of b cells of pancreas induced by high fructose and the potential mechanism related to up-regulation of PDX-1 (Qi et al 2010).…”

Section: Anti-parasitic Effectsmentioning

confidence: 99%

The pharmacology, toxicology and potential applications of arecoline: a review

Liu

Peng

et al. 2016

Pharmaceutical Biology

114

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Context: Arecoline is an effective constituent of Areca catechu L. (Arecaceae) with various pharmacological effects. However, investigations also revealed that long use of arecoline could arouse some oral diseases. Objective: The present review gathers the fragmented information available in the literature (before 1 October 2015) regarding pharmacology and toxicology of arecoline. We also discussed the potential developments and applications of arecoline in the future. Methods: All the available information regarding the arecoline is compiled from scientific databases, including Science Direct, PubMed, Web of Science, Scopus, etc. Results: Previous research demonstrated that arecoline is one of the major effective constituents in A. catechu. Additionally, arecoline has a wide spectrum of pharmacological activities including effects on nervous, cardiovascular, digestive and endocrine systems and anti-parasitic effects. What's more, arecoline is reported to be the primary toxic constituent of A. catechu, and the main toxic effects include oral submucous fibrosis (OSF), oral squamous cell carcinoma (OSCC) and genotoxicity. Conclusion: Arecoline has great potential to be a therapeutic drug for various ailments. However, further investigations are needed in the future to reduce or eliminate its toxicities before developing into new drug. ARTICLE HISTORY

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Section: Anti-parasitic Effectsmentioning

confidence: 99%

The pharmacology, toxicology and potential applications of arecoline: a review

Liu

Peng

et al. 2016

Pharmaceutical Biology

114

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“…In some experiments, adipocytes were cultured for 25 h with specific inhibitors of kinases (c-Jun NH 2 -terminal protein kinase (JNK), SP600125; p38 mitogen-activated protein kinase (p38MAPK), SB203580; Extracellular Signal-Regulated kinases 1/2 (ERK1/2), PD 98059) or of adenylate cyclase (MDL-12,330A), before the addition of ACEA or SR141716 for the last 24 h. All inhibitors were from Calbiochem (La Jolla, CA, USA); the concentrations used were based on those recommended by the manufacturer or previously published in the literature. [21][22][23][24] Because SR141716, SP600125, SB203580 and PD 98059 were dissolved in dimethylsulfoxide and ACEA was dissolved in ethanol, dimethylsulfoxide or ethanol was used as vehicle for the control conditions. The final concentrations of dimethylsulfoxide or ethanol in medium were o0.1%.…”

Section: Cell or Adipose Tissue Culturementioning

confidence: 99%

Endocannabinoids regulate adipokine production and the immune balance of omental adipose tissue in human obesity

Ge¹,

Maury²,

Rycken³

et al. 2012

Int J Obes

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OBJECTIVES: (1) To investigate whether modulation of the cannabinoid type 1 receptor (CB1R) directly regulates the production of adiponectin (ApN) and other adipokines in omental adipose tissue (OAT) of obese subjects, (2) to establish in which cellular fraction of OAT the effects of CB1R blockade take place and (3) to unravel the underlying mechanisms. SUBJECTS AND METHODS: OAT was obtained from 30 obese subjects (body mass index: 40.6±1.3 kg m À 2 ) undergoing abdominal surgery. Primary cultures of explants or of freshly isolated adipocytes or stromal-vascular cells (SVCs) were used. RESULTS: In OAT explants, the CB1R blocker Rimonabant upregulated ApN gene expression. mRNA abundance of omentin that exhibits insulin-sensitizing properties was upregulated as well. Conversely, mRNA levels of two pro-inflammatory cytokines, macrophage inflammatory protein (MIP)-1b and interleukin (IL)-7 were downregulated. We next examined where these effects took place within OAT. CB1R expression was similar in both cellular fractions. In isolated mature adipocytes, blockade of CB1R reproduced the increase of ApN mRNA and the decrease of IL-7 mRNA, while inducing a rise of ApN secretion into the medium. In isolated SVC, gene expression of omentin, which is restricted to this fraction, was augmented, while that of MIP-1b was diminished. Finally, we deciphered the mechanisms leading to ApN regulation by the endocannabinoid system (ES). We first established that ApN regulation was actually mediated by the CB1R: ApN gene expression was upregulated by Rimonabant and downregulated by the CB1R agonist arachidonyl-2-chloroethylamide (ACEA). Upregulation of ApN by Rimonabant was unaltered by inhibiting cAMP production. However, downregulation of ApN by ACEA was fully reversed by an inhibitor of p38 mitogen-activated protein kinase (p38MAPK) and ACEA increased p38MAPK phosphorylation. CONCLUSIONS: Blockade of CB1R attenuates the inflammatory state in both cellular fractions of OAT either by increasing ApN and omentin production or by decreasing mRNAs of MIP-1b and IL-7. ApN regulation by the ES partly involves p38MAPK.

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“…During the period, culture medium was changed every 2 days. Following the treatments, cells were stained with Oil Red O solution as previously described in detail (Hsu et al 2010). …”

Section: Adipogenic Differentiation Of Mouse 3t3-l1 Cellsmentioning

confidence: 99%

A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens

Wang

Chao

et al. 2010

Biotechnol Lett

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A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity.

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Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes—A possible role of betel-quid chewing in metabolic syndrome

Cited by 61 publications

References 47 publications

The pharmacology, toxicology and potential applications of arecoline: a review

The pharmacology, toxicology and potential applications of arecoline: a review

Endocannabinoids regulate adipokine production and the immune balance of omental adipose tissue in human obesity

A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens

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