Effects of Arginine Deprivation on Polyoma Virus Infection of Mouse Embryo Cultures

Winters, Alvin L.; Consigli, Richard A.

doi:10.1099/0022-1317-10-1-53

Cited by 23 publications

(6 citation statements)

References 11 publications

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“…Replication of a number of DNA viruses including human adenoviruses has been shown to be sensitive to the absence of arginine from the medium (Becker et al 1967, Goldblum et al 1968, Rouse et al 1963, Tankersley 1964, Winters & Consigli 1971. In the present experiments this was found to be the case also with SA7 virus, although the block was not complete as shown by a slight increase in the titre of infectious virus.…”

Section: Discussionsupporting

confidence: 50%

GROWTH OF SIMIAN ADENOVIRUS SA₇ DURING ARGININE STARVATION

1972

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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The effect of arginine starvation on the growth of simian adenovirus SA7 has been studied. Omission of arginine from the medium inhibited production of infectious virus. Extracts from virus-infected cells maintained in arginine-free medium were tested for immature virus particles. Small amounts of virus activity found in these extracts seemed to be caused by the low levels of normal virus present, rather than by defective forms of virus.

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Section: Discussionsupporting

confidence: 50%

GROWTH OF SIMIAN ADENOVIRUS SA₇ DURING ARGININE STARVATION

1972

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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“…If macrophage-produced arginase turns out to be important in herpes simplex virus infections in vivo, we may be sure that other viruses will be affected in the same way. Arginine is essential for the production of poxviruses (2), adenoviruses (24), and papovaviruses (28). We have evidence that vaccinia virus is indeed restricted in culture by macrophages; an experiment done in the same way as that illustrated in Fig.…”

Section: Discussionmentioning

confidence: 81%

Inhibition of herpes simplex virus multiplication by activated macrophages: a role for arginase?

Wildy¹,

Gell²,

Rhodes³

et al. 1982

Infect Immun

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Proteose-peptone-activated mouse macrophages can prevent productive infection by herpes simplex virus in neighboring cells in vitro whether or not those cells belong to the same animal species. The effect does not require contact between the macrophages and the infected cells, may be prevented by adding extra arginine to the medium, and may be reversed when extra arginine is added 24 h after the macrophages. Arginase activity was found both intracellularly and released from the macrophages. The extracellular enzyme is quite stable; 64% activity was found after 48 h of incubation at 37°C in tissue culture medium. No evidence was found that the inefficiency of virus replication in macrophages was due to self-starvation by arginase. As might be predicted macrophages can, by the same mechanism, limit productive infection by vaccinia virus.

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“…Since Rouse et al (22) discovered the effect of arginine on the growth of adenovirus in KB cells, there have been a number of reports of similar phenomena with different mammalian virus and host cell systems (1,2,5,8,15,16,21,26,27). Arginine is required for cell growth and virus production in A. albopictus C6/36 cells as shown in this report.…”

Section: Discussionmentioning

confidence: 72%

Amino Acid Requirements for the Growth of Aedes albopictus Clone C6/36 Cells and for the Production of Dengue and Chikungunya Viruses in the Infected Cells

Sasao

Igarashi

Fukai

1980

Microbiology and Immunology

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Amino acid requirements for the growth of Aedes albopictus, clone C6/36, cells and for the production of dengue (DEN) and Chikungunya (CHIK) viruses were examined by growing the cells or the viruses in media which were deprived of one of the 20 amino acids. Cell growth was markedly inhibited when cystine was omitted from the medium, and to a lesser extent by arginine deprivation. On the other hand, omission of alanine, asparagine, aspartic acid, and glutamic acid at the same time did not affect cell growth.Marked accumulation of alanine was observed in the medium when the cells were grown for 8 days in complete medium, with concomitant depletion of aspartic acid and glutamic acid. The production of CHIK virus was inhibited markedly by omission of cystine from the medium after virus infection, while the production of DEN viruses was more affected by glycine deprivation, although cystine deprivation also inhibited virus production to a lesser extent. On the other hand, production of CHIK and DEN viruses was not affected when alanine, asparagine, aspartic acid, and glutamic acid were omitted from the medium at the same time.Dengue (DEN) virus (a flavivirus, family Togaviridae) and Chikungunya (CHIK) virus (an alphavirus, family Togaviridae) are mosquito-borne arboviruses (3, 4), and infections with these viruses are important public health problems in Southeast Asia (10, 11). Studies on the growth characteristics and modes of existence of these viruses in vector mosquitoes are required to understand the basic principles of their ecology and the epidemiology of the diseases caused by the viruses. We have been studying the growth of these viruses in cultured mosquito cells, which can be considered a simplified system comparable to the vector mosquitoes. Recently, a clone, C6/36, (12) was isolated from a line of Singh's Aedes albopictus cells (23), and was shown to be more sensitive to DEN and CHIK viruses than the original uncloned cells.

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Effects of Arginine Deprivation on Polyoma Virus Infection of Mouse Embryo Cultures

Cited by 23 publications

References 11 publications

GROWTH OF SIMIAN ADENOVIRUS SA₇ DURING ARGININE STARVATION

GROWTH OF SIMIAN ADENOVIRUS SA₇ DURING ARGININE STARVATION

Inhibition of herpes simplex virus multiplication by activated macrophages: a role for arginase?

Amino Acid Requirements for the Growth of Aedes albopictus Clone C6/36 Cells and for the Production of Dengue and Chikungunya Viruses in the Infected Cells

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Effects of Arginine Deprivation on Polyoma Virus Infection of Mouse Embryo Cultures

Cited by 23 publications

References 11 publications

GROWTH OF SIMIAN ADENOVIRUS SA7 DURING ARGININE STARVATION

GROWTH OF SIMIAN ADENOVIRUS SA7 DURING ARGININE STARVATION

Inhibition of herpes simplex virus multiplication by activated macrophages: a role for arginase?

Amino Acid Requirements for the Growth of Aedes albopictus Clone C6/36 Cells and for the Production of Dengue and Chikungunya Viruses in the Infected Cells

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GROWTH OF SIMIAN ADENOVIRUS SA₇ DURING ARGININE STARVATION

GROWTH OF SIMIAN ADENOVIRUS SA₇ DURING ARGININE STARVATION