1993
DOI: 10.1055/s-2007-1002099
|View full text |Cite
|
Sign up to set email alerts
|

Effects of Arterial-Portal Glucose Difference on Gluconeogenesis from Lactate in the Isolated Bivascular-Perfused Rat Liver

Abstract: The effects of arterial-portal glucose difference on the gluconeogenesis from lactate were studied using the bivascular perfused liver isolated from the fasted rat. The liver was cyclically perfused at flow rates of 14 ml/min from the portal vein and of 7 ml/min from the hepatic artery with the total volume of 35 ml of perfusion medium containing 2 mM glucose, 3 mM lactate and (U-14C)-lactate for 20 min. Glucose was infused at a rate of 27.75 mumol/min into the arterial cannula (A-experiment) or the portal can… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
4
0

Year Published

1997
1997
2013
2013

Publication Types

Select...
6

Relationship

2
4

Authors

Journals

citations
Cited by 6 publications
(4 citation statements)
references
References 12 publications
0
4
0
Order By: Relevance
“…Incorporation of 14 C lactate into glucose in perfusion was assayed according to a previously reported method [9]. Briefly, perfusate was deproteinized and boiled to liberate CO 2 gas.…”
Section: Gluconeogenesismentioning
confidence: 99%
“…Incorporation of 14 C lactate into glucose in perfusion was assayed according to a previously reported method [9]. Briefly, perfusate was deproteinized and boiled to liberate CO 2 gas.…”
Section: Gluconeogenesismentioning
confidence: 99%
“…The disposal of this excess glucose falls largely on the white adipose tissue (WAT), which is already strained by the excess lipid [7], thereby favoring glycolysis and lactate production [21]. The excess lactate is used by the liver for gluconeogenesis [22] or lipid synthesis [23] (Fig. 1).…”
Section: The Pathognomic Links: Insulin Resistancementioning
confidence: 99%
“…Pancreas and liver were perfused according to the previously reported methods (Mokuda and Sakamoto, 1998;Mokuda et al, 1993). The pancreas was perfused at a rate of 4 ml/min.…”
Section: Pancreas and Liver Perfusionmentioning
confidence: 99%
“…Insulin values were determined by the use of a rat insulin standard (Cosmo Bio, Tokyo) and the other components of a radioimmunoassay kit (Shionogi, Osaka) with its intra-and interassay CV of 5.8% and 5.2%. Perfusate glucose-1-14 C was measured by the previously reported method (Mokuda et al, 1993). Briefly, perfusate was deproteinized and boiled to liberate CO 2 gas.…”
Section: Analysesmentioning
confidence: 99%