Effects of ascorbate fatty ester derivatives on erythrocyte membrane lipoperoxidation

Abstract

6-O-alkyl ascorbic acid esters (ASC n ) are amphiphilic molecules that behave as surfactants in aqueous solution. ASC n have shown some physical and rheological properties that suggest a potential utility as drug carriers.The present paper aims to evaluate the effect of ASC n on erythrocyte properties in order to get information regarding the relationship between osmotic fragility, erythrocyte deformability and membrane lipoperoxidation process. The assays were performed at the following concentrations: the critical micelar concentration (CMC), producing 10% hemolysis (CH 10 ) and producing 50% hemolysis (CH 50 ). We observed that ASC n (ASC 8 , ASC 10 and ASC 12 ), at concentration nearby CMC, neither affected cell deformability nor produced lipoperoxidation. Nevertheless, at higher concentrations (CH 10 and CH 50 ), the RBCs incubated with ASC n were affected by a significant and progressive loss of deformability, simultaneously with an increase of osmotic fragility and membrane lipoperoxidation.

Hemorheological consequences of hind limb ischemia-reperfusion differs in normal and gonadectomized male and female rats

“…The reactive free radicals cause direct and cascade-like harmful effects, including lipid peroxidation of the membrane, methemoglobin and Heinz-body formation -so increasing the inner viscosity of the cells as well as protein modification (e.g. by sulfhydryl cross-linking) -and consequent functional changes in transmembrane proteins, ion channels, structural proteins [4,8,13,34,45]. Changes in micro-environmental conditions (e.g.…”

Podávanie vitamínu C zlepšuje reológiu plnej krvi u zdravých ľudí / Vitamin C supplementation promotes whole blood rheology in healthy humans

“…Všetky uvedené parametre opísané v našej štúdii sme merali pri monitorovaní vybraných parametrov antioxidačného stavu a oxidačného stresu v plazme. Tieto analýzy boli nevyhnutné, keďže vitamín C sme podávali v pomerne vysokej dávke (1 000 mg/denne), ktorá by mohla mať aj prooxidač- ný účinok a spôsobiť poškodenie erytrocytov stimuláciou peroxidácie lipidov (43). Napriek tomu sme v aktuálnej, ako aj v už publikovanej štúdii (19) nepozorovali žiadny vzťah medzi zmenami charakteristík erytrocytov a parametrami antioxidačného stavu v plazme.…”