Effects of Ascorbic Acid on Proliferation and Collagen Synthesis in Relation to the Donor Age of Human Dermal Fibroblasts

Phillips, Charlotte L.; Combs, S.; Pinnell, Sheldon R.

doi:10.1111/1523-1747.ep12393187

Cited by 176 publications

(128 citation statements)

References 28 publications

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“…The hydroxylation of proline involves the activation of a reactive iron-oxygen complex, and ascorbic acid is required for the reduction of Fe 3ϩ formed in this reaction (25). Previous studies have shown that the addition of ascorbic acid stimulates collagen production in many metazoans (26)(27)(28)(29)(30)(31)(32); however, its effect on collagen production in basal metazoans such as corals is not known. The addition of ascorbic acid to the culture media increased collagen production by Ϸ40% in M. digitata cell cultures (4.4 Ϯ 0.2 weight percent collagen relative to total protein without ascorbic acid compared with 6.2 Ϯ 0.5% with ascorbic acid; P Յ 0.01; Fig.…”

Section: Resultsmentioning

confidence: 99%

Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro

Helman¹,

Natale²,

Sherrell

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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The evolution of multicellularity in animals required the production of extracellular matrices that serve to spatially organize cells according to function. In corals, three matrices are involved in spatial organization: (i) an organic ECM, which facilitates cell-cell and cell-substrate adhesion; (ii) a skeletal organic matrix (SOM), which facilitates controlled deposition of a calcium carbonate skeleton; and (iii) the calcium carbonate skeleton itself, which provides the structural support for the 3D organization of coral colonies. In this report, we examine the production of these three matrices by using an in vitro culturing system for coral cells. In this system, which significantly facilitates studies of coral cell physiology, we demonstrate in vitro excretion of ECM by primary (nondividing) tissue cultures of both soft (Xenia elongata) and hard (Montipora digitata) corals. There are structural differences between the ECM produced by X. elongata cell cultures and that of M. digitata, and ascorbic acid, a critical cofactor for proline hydroxylation, significantly increased the production of collagen in the ECM of the latter species. We further demonstrate in vitro production of SOM and extracellular mineralized particles in cell cultures of M. digitata. Inductively coupled plasma mass spectrometry analysis of Sr/Ca ratios revealed the particles to be aragonite. De novo calcification was confirmed by following the incorporation of 45 Ca into acid labile macromolecules. Our results demonstrate the ability of isolated, differentiated coral cells to undergo fundamental processes required for multicellular organization.aragonite ͉ cell culture ͉ cnidaria ͉ calcification ͉ C orals (class, Anthozoa) are the most basal cnidarians and the first animal phylum with an organized neural system and complex active behavior (1). The embryonic gastrula develops to form an outer ectoderm and an inner endoderm separated by the mesoglea, a noncellular fibrous jelly-like material (2). The two germ layers are spatially structured by an ECM in which embedded, interstitial (stem) cells give rise to nematocysts, mucous glands, and sensory or nerve cells (2, 3). Many corals also precipitate calcium carbonate in the form of aragonite on a skeletal organic matrix (SOM) template (4, 5). The precipitation pattern is highly controlled between colonies, giving rise to morphological structures that are used as primary phenotypic markers of species in extant reefs and fossil samples.The basic cellular processes responsible for the production of ECM, SOM, and calcium carbonate skeleton remain largely unknown. Molecular, genetic, and physiological analyses of cellular processes in corals have been elusive mainly because it is difficult to grow corals under controlled conditions in the laboratory and because of the genetic and physiological complexities inherent in associations of the animals with symbionts and parasites. All zooxanthellate corals harbor intracellular symbiotic dinoflagellates (zooxanthellae) within their endoderm cells; the al...

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Section: Resultsmentioning

confidence: 99%

Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro

Helman¹,

Natale²,

Sherrell

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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“…52 On day 14, AA had no significant effect on increasing the cell proliferation. This was probably due to the fact that the cells concentrated on ECM synthesis and deposition and stopped proliferating.…”

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confidence: 91%

The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering

Bačáková¹,

Musı́lková²,

Riedel³

et al. 2016

IJN

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Fibrin plays an important role during wound healing and skin regeneration. It is often applied in clinical practice for treatment of skin injuries or as a component of skin substitutes. We prepared electrospun nanofibrous membranes made from poly( l -lactide) modified with a thin fibrin nanocoating. Fibrin surrounded the individual fibers in the membrane and also formed a thin fibrous mesh on several places on the membrane surface. The cell-free fibrin nanocoating remained stable in the cell culture medium for 14 days and did not change its morphology. On membranes populated with human dermal fibroblasts, the rate of fibrin degradation correlated with the degree of cell proliferation. The cell spreading, mitochondrial activity, and cell population density were significantly higher on membranes coated with fibrin than on nonmodified membranes, and this cell performance was further improved by the addition of ascorbic acid in the cell culture medium. Similarly, fibrin stimulated the expression and synthesis of collagen I in human dermal fibroblasts, and this effect was further enhanced by ascorbic acid. The expression of beta 1 -integrins was also improved by fibrin, and on pure polylactide membranes, it was slightly enhanced by ascorbic acid. In addition, ascorbic acid promoted deposition of collagen I in the form of a fibrous extracellular matrix. Thus, the combination of nanofibrous membranes with a fibrin nanocoating and ascorbic acid seems to be particularly advantageous for skin tissue engineering.

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“…There is much evidence on the regulation of these functions by the addition of various cytokines to cell cultures. TGF-β, TNF-α, and IFN-γ may inhibit or stimulate production of dermal extracellular matrix proteins and collagenase enzyme (14)(15)(16)(17).…”

Section: Discussionmentioning

confidence: 99%

Investigation of joint hypermobility in individuals with hyperbilirubinemia

et al. 2017

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Objective: Benign joint hypermobility syndrome refers to hypermobile individuals with musculoskeletal symptoms in the absence of any systemic rheumatic disease; its prevalence is approximately 0.5%. In animal studies, bilirubin has been shown to reduce fibrosis induced by bleomycin. It has been suggested that bilirubin leads to hypermobility that affects the structure or function of collagen. In addition, our observation is that hypermobility occurs more often in patients with indirect hyperbilirubinemia. In this study, we aimed to evaluate hypermobility in patients with indirect hyperbilirubinemia. Material and Methods:We recruited 120 consecutive patients with indirect hyperbilirubinemia from a tertiary gastroenterology outpatient clinic and examined them for hypermobility. Hypermobility was evaluated using the Beighton criteria, and other relevant clinical findings were recorded. In addition, a group of healthy individuals (n=107) without hyperbilirubinemia were included as controls.Results: The mean ages of the patients and controls were 33.4±12.9 and 36.2±11.2 years, respectively (p=0.09). In total, 100 (83%) patients and 78 (73%) controls were male (p=0.075). The mean indirect bilirubin levels were 1.44±0.66 mg/dL in the patient group and 0.37±0.18 mg/dL in the control group. Based on the Beighton score, 23 patients (19.2%) in the patient group and 3 (2.8%) individuals in the control group had joint hypermobility. The difference between the groups was statistically significant (p<0.001).Conclusion: According to the results of our study, findings of joint hypermobility are more frequent in patients with indirect hyperbilirubinemia than in controls.

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Effects of Ascorbic Acid on Proliferation and Collagen Synthesis in Relation to the Donor Age of Human Dermal Fibroblasts

Cited by 176 publications

References 28 publications

Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro

Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro

The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering

Investigation of joint hypermobility in individuals with hyperbilirubinemia

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