Effects of aspirin on expression of iron transport and storage proteins in BV-2 microglial cells

Xu, Yan; Du, Fang; Li, Jiang; Gong, Jianping; Zhou, Yu‐Fu; Luo, Qian; Qian, Zhong Ming; Ke, Ya

doi:10.1016/j.neuint.2015.10.014

Cited by 17 publications

(16 citation statements)

References 24 publications

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“…However, the viability of the cells treated with 0.1 mM aspirin plus LPS was significantly higher than that of the cells treated with LPS alone and almost the same as that of control cells (Figure 1). This result is consistent with our recent findings [18] and re-confirmed that aspirin has a role in protecting BV-2 microglial cells from LPS-induced damage in vitro.…”

Section: Resultssupporting

confidence: 94%

“…We used 0.1 mM aspirin because this concentration was found to have a significant effect on hepcidin mRNA expression in LPS-treated BV-2 microglial cells in another recent study [18]. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that there was no significant difference in cell viability between cells treated with the vehicle (control) or with 0.1 mM aspirin (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…In our recent study, we [18] demonstrated that aspirin significantly inhibits the LPS-induced increase in IL-6 and hepcidin mRNA expression and revises the LPS-evoked reduction in TfR1 and Fpn1 expression in BV-2 microglial cells. The inhibition of aspirin on IL-6 and hepcidin mRNA expression suggests that aspirin might have play a role in suppressing the activated IL-6/ JAK2/STAT3 signaling pathway in LPS-treated BV-2 microglial cells.…”

Section: Discussionmentioning

confidence: 99%

“…Then, BV-2 cells were seeded in 96-well plates (6 × 10 3 cells/well) for cell viability assay, and 6-well plates (4 × 10 5 cells/well) for RT-PCR and (8 × 10 5 cells/well) for Western blotting analysis [18]. The cells were treated with different concentrations of 0.1 mM aspirin (in 0.1% ethanol) and/or LPS (1 mg/mL in PBS), which were dissolved in fresh DMEM without serum.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we therefore investigated the effects of aspirin on the expression of three major iron metabolism proteins, transferrin receptor 1 (TfR1), ferroportin 1 (Fpn1), and ferritin, as well as hepcidin and interleukin 6 (IL-6) in BV-2 microglial cells. We found that aspirin significantly down regulates TfR1 and up regulates Fpn1 and ferritin expressions in cells treated without lipopolysaccharides (LPS) in vitro, as well as down regulates hepcidin and IL-6 levels in cells treated with LPS [18]. However, the relevant mechanisms are unknown.…”

Section: Introductionmentioning

confidence: 99%

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Aspirin down Regulates Hepcidin by Inhibiting NF-κB and IL6/JAK2/STAT3 Pathways in BV-2 Microglial Cells Treated with Lipopolysaccharide

Zhou

et al. 2016

IJMS

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Aspirin down regulates transferrin receptor 1 (TfR1) and up regulates ferroportin 1 (Fpn1) and ferritin expression in BV-2 microglial cells treated without lipopolysaccharides (LPS), as well as down regulates hepcidin and interleukin 6 (IL-6) in cells treated with LPS. However, the relevant mechanisms are unknown. Here, we investigate the effects of aspirin on expression of hepcidin and iron regulatory protein 1 (IRP1), phosphorylation of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3) and P65 (nuclear factor-κB), and the production of nitric oxide (NO) in BV-2 microglial cells treated with and without LPS. We demonstrated that aspirin inhibited hepcidin mRNA as well as NO production in cells treated with LPS, but not in cells without LPS, suppresses IL-6, JAK2, STAT3, and P65 (nuclear factor-κB) phosphorylation and has no effect on IRP1 in cells treated with or without LPS. These findings provide evidence that aspirin down regulates hepcidin by inhibiting IL6/JAK2/STAT3 and P65 (nuclear factor-κB) pathways in the cells under inflammatory conditions, and imply that an aspirin-induced reduction in TfR1 and an increase in ferritin are not associated with IRP1 and NO.

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Section: Resultssupporting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Aspirin down Regulates Hepcidin by Inhibiting NF-κB and IL6/JAK2/STAT3 Pathways in BV-2 Microglial Cells Treated with Lipopolysaccharide

Zhou

et al. 2016

IJMS

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Different Characteristics of Hepcidin Expression in IL-6+/+ and IL-6−/− Neurons and Astrocytes Treated with Lipopolysaccharides

Zhang

et al. 2018

Neurochem Res

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A region-specific regulation of inflammation on the expression hepcidin in the brain has been demonstrated, however, it remains unknown whether there is also a cell-specific regulation of inflammation on hepcidin in the brain. Here, we investigated the effects of lipopolysaccharides (LPS) on the expression of hepcidin mRNA and also the expression of IL-6 mRNA, the phosphorylation of STAT3 and the expression of ferroportin 1 (Fpn1) and ferritin light chain (Ft-L) proteins in neurons and astrocytes obtained from wild type (IL-6+/+) and IL-6 knockout (IL-6-/-) mice. We demonstrated that the responses of the expression of hepcidin and IL-6 mRNAs, the phosphorylation of STAT3, and the expression of Fpn1 protein to LPS in IL-6+/+ astrocytes and also the responses of the expression of hepcidin mRNA, the phosphorylation of STAT3 and the expression of Fpn1 protein to IL-6 in IL-6-/- astrocytes were much stronger than those in IL-6+/+ and IL-6-/- neurons. A significant increase in Ft-L was found in LPS-treated IL-6+/+ and IL-6-treated IL-6-/- astrocytes, but not in LPS-treated IL-6+/+ and IL-6-treated IL-6-/- neurons. Our findings provide in vitro evidence for the existence of a cell-specific regulation of LPS on the expression of hepcidin and also Ft-L in the brain.

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Ferroportin1 in the brain

Qian

Guo

2023

Ageing Research Reviews

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Effects of aspirin on expression of iron transport and storage proteins in BV-2 microglial cells

Cited by 17 publications

References 24 publications

Aspirin down Regulates Hepcidin by Inhibiting NF-κB and IL6/JAK2/STAT3 Pathways in BV-2 Microglial Cells Treated with Lipopolysaccharide

Aspirin down Regulates Hepcidin by Inhibiting NF-κB and IL6/JAK2/STAT3 Pathways in BV-2 Microglial Cells Treated with Lipopolysaccharide

Different Characteristics of Hepcidin Expression in IL-6+/+ and IL-6−/− Neurons and Astrocytes Treated with Lipopolysaccharides

Ferroportin1 in the brain

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