Effects of assay medium composition on macrophage-mediated tumor cell binding and lysis

Hasday, Jeffrey D.; Crawford, Eric K.

doi:10.1016/0022-1759(88)90180-9

Cited by 7 publications

(2 citation statements)

References 27 publications

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“…Macrophages modify their properties in response to their specific microenvironment, and therefore differences in culture conditions can also result in stark variations in functional output. Within the literature summarised in Table 2, variations in assay media (e.g., presence of serum) and cell density are present, both of which are noted to influence macrophage phenotypes and responses heavily [190][191][192]. Moreover, the inflammatory stimuli differ between studies, and as noted earlier, this can contribute to different inflammatory outcomes.…”

Section: Discussionmentioning

confidence: 95%

The Immunomodulatory Effects of Statins on Macrophages

Sheridan

Wheeler‐Jones

Gage

2022

Immuno

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Statins are 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors used worldwide to manage dyslipidaemia and thus limit the development of atherosclerotic disease and its complications. These atheroprotective drugs are now known to exert pleiotropic actions outside of their cholesterol-lowering activity, including altering immune cell function. Macrophages are phagocytic leukocytes that play critical functional roles in the pathogenesis of atherosclerosis and are directly targeted by statins. Early studies documented the anti-inflammatory effects of statins on macrophages, but emerging evidence suggests that these drugs can also enhance pro-inflammatory macrophage responses, creating an unresolved paradox. This review comprehensively examines the in vitro, in vivo, and clinical literature to document the statin-induced changes in macrophage polarization and immunomodulatory functions, explore the underlying mechanisms involved, and offer potential explanations for this paradox. A better understanding of the immunomodulatory actions of statins on macrophages should pave the way for the development of novel therapeutic approaches to manage atherosclerosis and other chronic diseases and conditions characterised by unresolved inflammation.

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Section: Discussionmentioning

confidence: 95%

The Immunomodulatory Effects of Statins on Macrophages

Sheridan

Wheeler‐Jones

Gage

2022

Immuno

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“…The sera selected can be autologous (collected from the subject), pooled standard sera, or fetal calf serum. Since the sera and media used contain nutrients and other growth factors that influence the metabolism and function of cells, care must be taken in standardizing the conditions within and between experiments (Hasday and Crawford 1988). The use of serum-free media may be possible in some experiments.…”

Section: Functional Assaysmentioning

confidence: 99%

Using immunological techniques to determine the effect of nutrition on T-cell function

Field

1996

Can. J. Physiol. Pharmacol.

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It is accepted that nutrition is important in the development and function of the immune system. However, the study of nutritional immunology is a relatively new discipline. In part this may be due to difficulties in defining the effect of nutrition on immunity, as an immune response is the result of an orchestrated cascade involving many cell types. This paper reviews basic concepts in immunology and discusses some of the immunological methods available to answer nutrition questions. The review focuses on the application of methodologies designed to acquire, to describe, and to assess functionally the T-lymphocyte component of the cell-mediated immune function. Functional measurements in vivo (delayed type hypersensitivity response) and in vitro (lymphocyte blastogenesis and metabolism) and immune mediators (cytokine production) have been used to determine the effect of nutrition on lymphocytes. The challenge of the future is for nutritionists to utilize many of the new concepts and techniques in immunology to design experiments that will ultimately lead to defining diets to modulate immunocompetence in health and disease.

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Natural killer sensitivity of tumor cells isolated from primary and metastatic lesions of four Bomirski melanoma variants

et al. 1991

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Natural killer (NK) sensitivity of melanoma cells isolated from primary and metastatic lesions of four Bomirski melanoma variants was compared. The hamster melanomas differed in their growth rate and metastatic pattern. We found that during tumor growth of all the variants tested, NK sensitivity of melanoma cells at the metastasis formation stage was significantly lower in both primary and metastatic tumors than in cells isolated from primary tumors at transplantation. In the case of Ma, Ab and Ab-455, NK sensitivity of primary tumor cells was higher than that of the cells isolated from metastatic deposits. These data obtained from a spontaneous metastasis tumor model argue for the role of NK cells in preventing metastatic spread of Bomirski melanomas studied.

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Effects of assay medium composition on macrophage-mediated tumor cell binding and lysis

Cited by 7 publications

References 27 publications

The Immunomodulatory Effects of Statins on Macrophages

The Immunomodulatory Effects of Statins on Macrophages

Using immunological techniques to determine the effect of nutrition on T-cell function

Natural killer sensitivity of tumor cells isolated from primary and metastatic lesions of four Bomirski melanoma variants

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