Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFVin vitro

Abstract

BackgroundAstragalus polysaccharide (APS) has immunomodulatory activities on porcine peripheral blood mononuclear cells. The immunomodulatory effects of APS on porcine endothelial cells (ECs) expose to classical swine fever virus (CSFV) remain unknown.MethodsThe virus was titrated using an indirect immune biotin enzyme standard method to confirm that porcine ECs were susceptible to CSFV infection and to determine the TCID50 of CSFV (C-strain). Porcine ECs were cultured with CSFV in the presence of APS. Relative quantitative PCR was used to assess the mRNA expression of factors that influence EC adhesion and immunity.ResultsThe expression of adhesion factors mRNA increased following stimulation with CSFV; this effect was inhibited by pre-exposing the cells to APS. In addition, the expression of growth factors and some immune factors increased after infection with CSFV; this increase in tissue factor (TF), transforming growth factor (TGF-β), and interleukin-8 (IL-8) could be inhibited by the addition of APS. The immune response mediated by Toll-like receptor 4 (TLR4) in ECs may be unregulated by CSFV as it was also inhibited by pre-treatment with APS.DiscussionThe addition of APS to the culture can obviously regulate the expression of molecules related to the adhesion, growth, and immune response of ECs, as well as the production of cytokines. Therefore, it may have the potential to be an effective component in vaccines against CSFV.

Structural characterization and adjuvant action of Paulownia tomentosa flower polysaccharide on the immune responses to classical swine fever vaccine in mice

“…In recent years, research has mainly focused on polysaccharides obtained from Chinese medicinal herbs due to their value as novel immune adjuvants against infections ( 34 , 35 ). For example, Astragalus polysaccharides (APS) was evaluated for its potential as an effective adjuvant in vaccines against Porcine reproductive and respiratory syndrome virus (PRRSV) or CSFV, and demonstrated that APS had immunomodulatory effects on pig peripheral blood mononuclear cells exposed to PRRSV or CSFV ( 36 , 37 ). PTFP has been proven to enhance the immune effectiveness of Newcastle disease vaccine in chickens ( 21 ).…”

Astragalus Oral Solution Ameliorates Allergic Asthma in Children by Regulating Relative Contents of CD4+CD25highCD127low Treg Cells

“…Astragalus , a large genus of herbs, belongs to the legume family Fabaceae and has potent immune boosting and health-promoting properties ( 18 , 19 ). Astragalus membranaceus (AM) has been widely used for thousands of years in China to treat asthma.…”