Effects of atmospheric pressure plasma jet on the physicochemical, functional, and antioxidant properties of flaxseed protein

Abstract

The aim of this study was to explore the effect of atmospheric pressure plasma jet (APPJ) on the physicochemical, functional, and antioxidant properties of flaxseed protein following APPJ treatment (0 to 240 s). The results showed that the pH value continuously dropped with the minimum value of 3.45 ± 0.15 after 240 s of APPJ treatment (–61.7%, P < 0.05). The relative protein solubility significantly declined after 15 s of APPJ treatment (–43.1%, P < 0.05), which was accompanied by the evident increase in mean particle size of flaxseed protein in aqueous solution (+157%, P < 0.05). Moreover, the surface hydrophobicity and contents of disulfide bonds gradually raised when the APPJ exposure time extended from 30 to 240 s. Notably, the foaming, emulsifying, and in vitro antioxidant properties of flaxseed protein were significantly improved following short time of APPJ treatment (5 to 15 s), which was paralleled with the changes of spatial conformation, mild protein oxidation, as well as the release of phenolic acids and flavonoids from naturally occurring protein–phenolic complex. Our findings elucidated that APPJ may be considered as an effective strategy to improve the functionality and antioxidant activities of flaxseed protein.Practical ApplicationWe had evaluated the effect of APPJ treatment on the physicochemical, functional, and antioxidant properties of flaxseed protein, which was conducive to tailor flaxseed protein with the optimal techno‐functionality and antioxidant activities as a potential nano‐delivery vehicle.

The quality and antioxidant elucidation of germinated flaxseed treated with acidic electrolyzed water

“…The contents of total phenolic acids and flavonoids in flaxseed sprouts were determined by using the Folin–Ciocalteu colorimetric method and aluminum nitrate method, respectively (Yu et al., 2020 ). The results were expressed as milligram gallic acid equivalents (GAE) and rutin/100 g of flaxseed (dry basis), respectively.…”

“…The contents of total phenolic acids and flavonoids in flaxseed sprouts were determined by using the Folin-Ciocalteu colorimetric method and aluminum nitrate method, respectively (Yu et al, 2020).…”

Oxidative modifications and structural changes of human serum albumin in response to air dielectric barrier discharge plasma

“…The validated effects of plasma on proteins in solution can be roughly divided into two categories: oxidative modifications and the structural changes. Protein oxidation by plasma involves the modification of amino acid side chains [13][14][15][16], carbonylation [13,15,17], and disulfide bond cross-links [12,13,18,19]. The most pronounced side chain modifications involve the sulphonation of free sulfhydryl groups, which is also observed in treated amino acid solutions [20].…”

Comparative structural and techno‐functional elucidation of full‐fat and defatted flaxseed extracts: implication of atmospheric pressure plasma jet

“…It had been demonstrated that the solubility, emulsifying and foaming properties of flaxseed protein, peanut protein, myofibrillar protein and whey protein were differently optimized following plasma treatment. 11,12 Potentially, remodeling of the spatial conformation and mild oxidation of specific amino acid residues in proteins were induced because of the formation of reactive species upon suitable plasma exposure in aqueous medium. 13 Comparatively, the polymerization of xanthan gum in solid phase, as well as the depolymerization of chitosan in acetic acid aqueous solution, had been observed following dielectric barrier discharge and high voltage pulsed discharge plasma treatments, respectively, which further optimized the emulsifying stability and hydrophilicity of these polysaccharides.…”