Effects of atrazine on hepatic metabolism and endocrine homeostasis in rainbow trout (Oncorhynchus mykiss)

Salaberria, Iurgi; Hansen, Bjørn Henrik; Asensio, Vega; Olsvik, Pål A.; Andersen, Reidar; Jenssen, Bjørn Munro

doi:10.1016/j.taap.2008.09.023

Cited by 63 publications

(25 citation statements)

References 46 publications

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“…CAT activity in the experimental groups exposed to unspiked and spiked artificial sediments in the flood events did not differ significantly from the respective control groups, demonstrating that there were no alterations in the levels of this antioxidant defense enzyme by any of the treatments. CAT activities in control animals of 1.03±0.21 μmolmin -1 mg -1 similar to values from the current study have been reported by Salaberria et al (2009). However, the levels of lipid peroxides in the liver of exposed animals were significantly elevated compared to the control groups.…”

Section: Identification Of Suitable Biomarkers For Use In Coupled Hydsupporting

confidence: 91%

A combined hydraulic and toxicological approach to assess re-suspended sediments during simulated flood events. Part I–multiple biomarkers in rainbow trout

et al. 2010

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Purpose One of the central issues related to global changes in weather is the increasing occurrence of flood events that can result in the re-suspension of contaminated sediments in rivers. Here, we report on a proof-of-concept study combining hydraulic engineering and ecotoxicology in a new interdisciplinary approach to assess the toxicity of resuspended polluted sediments after a simulated flood event. Materials and methods Rainbow trout (Oncorhynchus mykiss) were exposed for 5 days under simulated flood conditions in an annular flume with artificial sediments that were spiked with a mixture of polycyclic aromatic hydrocarbons (PAH) at environmentally relevant concentrations.Specifically, the objective of this study was to bridge the gap between the physical re-suspension of pollutants and resulting toxicological impacts on aquatic organisms. A suite of different molecular, biochemical and histological markers was used to test the hypothesis that re-suspension of sediments can lead to re-mobilization of PAHs and subsequently to effects on aquatic organisms. Results and discussion The micronucleus frequency was significantly 4.3-fold elevated after exposure. There was no significant indication of Aryl hydrocarbon receptor signaling (no EROD induction or increased CYP1A protein content, only slight induction of CYP1A gene expression). Biliary metabolite concentration was the most sensitive Responsible editor:

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Section: Identification Of Suitable Biomarkers For Use In Coupled Hydsupporting

confidence: 91%

A combined hydraulic and toxicological approach to assess re-suspended sediments during simulated flood events. Part I–multiple biomarkers in rainbow trout

et al. 2010

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“…Liver is considered as the primary target organ of ATR and the major organ for CYP‐mediated biotransformation . ATR might exert a hepatotoxicity effect on the detoxification enzymes and nuclear xenobiotic receptors.…”

Section: Introductionmentioning

confidence: 99%

Activating nuclear xenobiotic receptors and triggering ER stress and hepatic cytochromes P450 systems in quails (Coturnix C. coturnix) during atrazine exposure

Qin

Lin

et al. 2017

Environmental Toxicology

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Atrazine (ATR) is one of the most widely detected contaminant in the ecosystem. Nuclear xenobiotic receptors are activated by herbicides and induce the transcription of CYP450 isoforms involved in xenobiotic metabolism and transport. However, little is known about hepatic nuclear xenobiotic receptors in birds are responsible for ATR-induced hepatotoxicity via regulating the cytochrome P450 enzyme systems (CYP450s). The objective of this study was to investigate the mechanism of ATR hepatotoxicity in quails. For this purpose, male quails were dosed by oral gavage from sexual immaturity to maturity with 0, 50, 250, and 500 mg/kg/day ATR for 45 days. The results showed that ATR exposure caused the hepatotoxicity damage and endoplasmic reticulum (ER) degeneration. It suggested that ER is a target organelle of ATR toxicity in hepatocytes. ATR exposure disrupted the hepatic CYP450s homeostasis. This study also demonstrated that ATR triggered the CYP450 isoforms transcription via activating the hepatic CAR/PXR pathway. The present study provides new insights regarding the mechanism of the ATR-induced hepatotoxicity through activating nuclear xenobiotic receptors and triggering ER stress and hepatic CYP450s in quails.

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“…Triazine molecules are able to enhance the concentration of reactive oxygen species (ROS) in fish (Elia et al 2002;Salaberria et al 2009;Jin et al, 2010;Xing et al 2012). Cells develop antioxidant defense system to scavenge the excessive ROS and maintain the redox balance such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST).…”

Section: Introductionmentioning

confidence: 99%

Melamine–cyanurate complexes and oxidative stress markers in trout kidney following melamine and cyanuric acid long-term co-exposure and withdrawal

Pacini

Dörr

Elia

et al. 2014

Fish Physiol Biochem

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In 2007, renal failure and death in pets were linked to pet food containing both melamine (MEL) and cyanuric acid (CYA). In mammals and fish, the co-administration of MEL and CYA causes renal crystal formation. Moreover, little is known about the process of crystal removal in fish. The aim of this study was to evaluate the formation of MEL-cyanurate crystals in kidney of rainbow trout (Oncorhynchus mykiss) fed combined MEL and CYA diets for 10 weeks at 250, 500 and 1,000 mg/kg in feed (equivalent to 2.5, 5, 10 mg/kg body weight of trout fed 1 % body weight per day). During the exposure trial and throughout a withdrawal period, prooxidant effects of MEL and CYA were evaluated on oxidative stress markers such as catalase, glutathione S-transferase and malondialdehyde. Crystal formation was dose and time dependent, and after six withdrawal weeks, crystals persisted in kidney of trout treated the highest triazine dose. Catalase and glutathione S-transferase activity in kidney of trout exposed to both triazines for 10 weeks indicated that MEL (with or without CYA) can exert a higher prooxidant effect than CYA dispensed singly. Although the enzymes activity increase appears to be reverted after two MEL withdrawal weeks, persistence of crystals may lead to severe damage in renal cells of fish.

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Effects of atrazine on hepatic metabolism and endocrine homeostasis in rainbow trout (Oncorhynchus mykiss)

Cited by 63 publications

References 46 publications

A combined hydraulic and toxicological approach to assess re-suspended sediments during simulated flood events. Part I–multiple biomarkers in rainbow trout

A combined hydraulic and toxicological approach to assess re-suspended sediments during simulated flood events. Part I–multiple biomarkers in rainbow trout

Activating nuclear xenobiotic receptors and triggering ER stress and hepatic cytochromes P450 systems in quails (Coturnix C. coturnix) during atrazine exposure

Melamine–cyanurate complexes and oxidative stress markers in trout kidney following melamine and cyanuric acid long-term co-exposure and withdrawal

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