Effects of Atropine on Acid Secretion by Isolated Frog Gastric Mucosa

Thorpe, Christian D.; Durbin, Richard P.

doi:10.1016/s0016-5085(72)80083-0

Cited by 13 publications

(10 citation statements)

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“…Since gastrin-stimulated acid secretion in the isolated stomach of the rat is inhibited by concentrations of atropine (0.1 mm and 1 mM) which are approximately 100-fold greater than those required (1-3 giM) to inhibit acetylcholinestimulated secretion, it appears unlikely that gastrin is stimulating acid secretion partly through a release of acetylcholine. For comparison, the present work is in agreement with some studies on the amphibian isolated gastric mucosa in which high concentrations of atropine at 2 mM (Thorpe & Durbin, 1972) and 10 mM (Nakajima et al, 1970) were required to inhibit pentagastrin-stimulated acid secretion, although Goto & Watanabe (1975) have reported that tetragastrinstimulated acid secretion is inhibited by 30 gM atropine in the isolated gastric mucosa ofthe frog. Histamine-stimulated acid secretion was not inhibited by high concentrations of atropine (1 mM) in this investigation.…”

Section: Discussionsupporting

confidence: 92%

“…Similar results have been obtained by other workers using concentrations of atropine ranging from 0.1 gM to 30 gM to inhibit acid secretion stimulated by cholinomimetic compounds in isolated gastric mucosa preparations of amphibians (Thorpe & Durbin, 1969Nakajima et al, 1970;Shoemaker, Makhlouf & Sachs, 1970;Goto & Watanabe, 1975). Arunlakshana & Schild (1959) have reported that a pAlo value for atropine of 4.2 (equivalent to a pA2 value of 5.15) was obtained on the frog rectus which was considered to possess nicotinic receptors.…”

Section: Discussionsupporting

confidence: 88%

“…Secondly, the plot of logl0 (DR-1) against log10 [antagonist] according to the equation for competitive inhibition was not linear. Comparable studies on mammalian isolated stomach or gastric mucosa preparations have not been reported, but it has been suggested by Thorpe & Durbin (1972) that atropine is a competitive inhibitor of acetylcholine in the amphibian isolated gastric mucosa preparation.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of atropine on acid secretion in isolated stomach preparations has not been extensively studied, although high concentrations of atropine (1-10 mM) have been used to inhibit histamineand pentagastrin-stimulated acid secretion in isolated gastric mucosa preparations of amphibians (Thorpe & Durbin, 1969Nakajima, Shoemaker, Hirschowitz & Sachs, 1970). In the present work the effect of atropine on acid secretion stimulated by histamine, gastrin and acetylcholine has been investigated in the isolated whole stomach of the rat in which both neural and hormonal influences have been minimized.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Effect of Atropine on Acid Secretion Stimulated by Acetylcholine, Histamine and Gastrin in the Isolated Whole Stomach of the Rat

Bunce¹,

Marsh²,

Parsons³

1977

British J Pharmacology

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An isolated stomach preparation from immature rats has been used to study the effect of atropine on gastric acid secretion. The acid secretory response to acetylcholine was not inhibited by atropine at a concentration of 0.3 μM. Concentrations of atropine of 1 to 3 μM produced a measurable inhibition of acid secretion, and a concentration of atropine of 10 μM caused a complete block of acid secretion which could not be surmounted by high concentrations of acetylcholine. The acid secretory response to histamine was not inhibited by concentrations of atropine of up to 1 mM. Concentrations of atropine of 1 μM and 10 μM did not inhibit gastrin‐stimulated acid secretion, although a significant inhibition of acid output was observed with atropine at concentrations of 0.1 mM and 1 mM. These findings are discussed in relation to the role of cholinergic mechanisms in the control of gastric acid secretion.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Effect of Atropine on Acid Secretion Stimulated by Acetylcholine, Histamine and Gastrin in the Isolated Whole Stomach of the Rat

Bunce¹,

Marsh²,

Parsons³

1977

British J Pharmacology

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“…Thus, atropine was used as an inhibitor of action of acetylcholine [20], burimamide as an 1-I 2 receptor blocker [1,19] and thiocyanate as an inhibitor at a more distal point.…”

Section: Acidificationmentioning

confidence: 99%

Acid secretion and intracellular pH in isolated oxyntic cells

Michelangeli

1978

J. Membrain Biol.

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This study demonstrates that isolated oxyntic cells are capable of secreting hydrochloric acid. Transitory peaks of medium acidification were observed when isolated oxyntic cells were stimulated with diffferent secretagogues. The duration of these peaks was of about 3 min and had a magnitude of about 0.5 muEquiv/hr mg dry weight. These peaks were abolished by selective inhibitors of acid secretion and/or secretagogue action. Cell pH, as measured by the DMO14C technique increases 0.13 pH units upon stimulation with db-cAMP. This change was abolished by pretreatment with SCN-. From acidification and cell pH experiments an increase of 5-10mM in base concentration inside the cell upon stimulation is calculated. These results are interpreted on the basis of the existence of a neutral anion exchange mechanism at the serosal side that serves to control the cell acid-base balance.

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