Effects of AttM lactonase on the pathogenicity ofStreptomyces scabies

Abstract

This study provides an efficient means to introduce the lactonase gene attM from Agrobacterium tumefaciens into Streptomyces scabies for evaluating the role of γ-butyrolactone (GBL) in thaxtomin A production and pathogenicity, etc. Our results showed that pathogenicity was abrogated in attM-expressing S. scabies exconjugants. Although there are gene knockout approaches to inactivating GBL signalling and thus pathogenicity in S. scabies, they are not only time consuming due to refractory host but also possibly incomplete in view of gene redundancy. Our work is the first report for a kind of lactonase affecting pathogenicity and/or virulence of scab-causing Streptomyces species.

Micronutrients and Soil Microorganisms in the Suppression of Potato Common Scab

“…No disease symptoms in planta, altered morphological differentiation-quorum quenching paralyzing γ-butyrolactone signaling pathway [161] The variation in the microbial community response might be connected to the mechanisms of suppression provided by the inoculated strains. Mostly, production of antibiotics against the pathogens is involved, while the identified compounds include geldanamycin [166], phenazine-1-carboxylic acid [163,164], isatropolone C [4], azalomycin [145] or antimicrobial peptides [154,165].…”

“…Mostly, production of antibiotics against the pathogens is involved, while the identified compounds include geldanamycin [166], phenazine-1-carboxylic acid [163,164], isatropolone C [4], azalomycin [145] or antimicrobial peptides [154,165]. However, the strains also produce compounds such as indole-3-acetic acid or siderophores and enable nitrogen fixation and phosphate solubilization [145] or remove signaling through γ-butyrolactone pathway [161].…”

Construct of Streptomyces Scabies Knock-Out Mutant of TXT Biosynthetic Gene txtB Via Intergeneric Conjugation

“…Streptomyces scabies strain 49173 was grown in the TSB agar (Elite Biotech. Co., Shanghai, China) as previously described [6]. Spore suspensions of Streptomyces strains were prepared as described before [7].…”

Heterologous expression of AHL lactonase AiiK by Lactobacillus casei MCJΔ1 with great quorum quenching ability against Aeromonas hydrophila AH-1 and AH-4

“…The purified AHL lactonases had poor resistance to environment when applied in a real situation. In the second case, AHL lactonases AiiA [ 22 ], AiiM [ 23 ], AttM [ 24 ], and HqiA [ 25 ] were expressed in different pathogens, and their pathogenicity decreased. This approach occurred only under ideal research conditions, because pathogens are difficult to be modified in true situation.…”

“…240B1 E. carotovora stain SCG1 The introduction of aiiA gene in E. carotovora stain SCG1 decreased extracellular pectolytic activities and pathogenicity of E. carotovora [ 22 ] AiiM AHL lactonase M. testaceum StLB037 P. c. c. NBRC 3830 The introduction of aiiM gene in P. carotovorum subsp. carotovorum NBRC 3830 attenuated soft rot symptoms on potato slices [ 23 ] AttM AHL lactonase A. tumefaciens c58 S. scabies The introduction of attM gene suppressed pathogenicity of S. scabies towards potato tuber [ 24 ] HqiA AHL lactonase Metagenomic library from soil P. c. c. CECT 225 T The introduction of hqiA gene in plant pathogen P. carotovorum efficiently interfered swarming motility and maceration enzymes production [ 25 ] Isolated and identified new QQ strains B. licheniformis T-1 AHL lactonase Freshwater culture pond sediment No B. licheniformis T-1 reduced pathogenicity of A. hydrophila cb15 in zebrafish coinjection [ 12 ] Constructed recombinant QQ strains Bb MomL AHL lactonase M. olearia Th120 B. brevis Bb MomL reduced secretion of pathogenic factors and pathogenicity of P. carotovorum subsp. carotovorum and P. aeruginosa PAO1 [ 26 ] Lc AiiK AHL lactonase K. huakuii L. casei MCJΔ1 Lc AiiK attenuated swimming motility, virulence factor production, and biofilm formation of A. hydrophila AH-1 and AH-4 (This study) …”