Effects of automated collection methods on semen quality and economic efficiency of boar semen production

Lellbach, Cord; Leiding, C.; Rath, D.; Staehr, B.

doi:10.1016/j.theriogenology.2008.06.048

Cited by 6 publications

(4 citation statements)

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“…The average bacterial burden determined for all farms in our study (82.4x10 3 CFU/mL in raw and 0.354x10 3 CFU/mL in diluted semen) is similar to that reported by others [13,41], and depends on the hygiene of semen collection and handling process and also on antibiotic resistance of the microbiota from a particular farm. Values are lower than those obtain by manual collection with double glove (384x10 3 CFU/mL) or by automatic collection (349x10 3 CFU/mL) [27]. Similar results have been published for other species.…”

Section: Discussionsupporting

confidence: 86%

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Ciornei¹,

Drugociu²,

Ciornei³

et al. 2021

Preprint

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The aim of the study is to establish the complete microbiological profile of boar semen and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pigs. More than one hundred semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by determining the degree of con-tamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149x103CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 x103CFU/mL. 23 bacterial and fungal species were isolated, the most common being Candida parapsilosis/sake (92%), Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm (by 49.85% in bacteria and by 9.67% in fungi). The load in bacteria and filamentous fungi can be con-trollable, but not in levuras fungi. In some Fluconazole-added extenders (12.5mg%), ensures the solution of this problem, and even increase in sperm progressivity (8.39%) for at least a 12-hour shelf life after dilution. The validation of the experiment was done by obtaining the sow fertility rate after AI.

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Section: Discussionsupporting

confidence: 86%

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Ciornei¹,

Drugociu²,

Ciornei³

et al. 2021

Preprint

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show abstract

“…The average bacterial burden determined for all farms in our study (82.4 × 10 3 CFU/mL in raw and 0.354 × 10 3 CFU/mL in diluted semen) was similar to that reported by others [ 5 , 20 ], and depended on the hygiene of semen collection and handling process and also on antibiotic resistance of the microbiota from a particular farm. Values were lower than those obtain by manual collection with double glove (384 × 10 3 CFU/mL) or by automatic collection (349 × 10 3 CFU/mL) [ 21 ]. Similar results have been published for other species.…”

Section: Discussionmentioning

confidence: 91%

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Ciornei¹,

Drugociu²,

Ciornei³

et al. 2021

Molecules

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The aim of the study was to establish the complete microbiological profile of boar semen (Sus scrofa domesticus) and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pig farms. One hundred and one semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by evaluating the degree of contamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149 × 103 CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 × 103 CFU/mL. Twenty-four germs (15 bacterial and 9 fungal species) were isolated, the most common being Candida parapsilosis/sake (92%) and Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm by 49.85% in bacteria (significant (p < 0.00001) and by 9.67% in fungi (non-significant (p < 0.111491). The load in bacteria and filamentous fungi can be controllable, but not in levuras fungi. Some fluconazole-added extenders (12.5 mg%), ensure fungal aseptization, and even an increase in sperm progressivity (8.39%) for at least a 12 h shelf life after dilution. Validation of sperm aseptization was done by maintaining sow fecundity unchanged after AI (insignificant p > 0.05).

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“…The rich fraction of the ejaculate is the base foundation of AI dose preparation. However, currently, boar studs are including semi-automatic ejaculate collectors [ 10 , 11 , 12 ] instead of the traditional manual gloved-hand method, where the entire ejaculate is collected. It is known that boar seminal plasma composition varies depending on the fraction [ 2 , 13 , 14 ], which influences sperm conservation.…”

Section: Introductionmentioning

confidence: 99%

Should All Fractions of the Boar Ejaculate Be Prepared for Insemination Rather Than Using the Sperm Rich Only?

Luongo

Llamas-López

Caravaca

et al. 2022

Biology

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Boar ejaculate is released in several well-characterized fractions, differing in terms of sperm concentration, seminal plasma volume, and composition. However, the inclusion of the last part of the ejaculate for artificial insemination (AI) purposes is still under debate due to its controversial effects. Thus, there is a need to study the potential synergistic impact of the different ejaculate fractions. We aimed to evaluate the effect of accumulative ejaculate fractions on sperm conservation, AI performance, and offspring health. Ejaculates (n = 51) were collected and distributed as follows: F1: sperm-rich fraction; F2: sperm-rich + intermediate fractions; F3: sperm-rich + intermediate + poor fractions. Each group was diluted in a commercial extender, packaged in seminal doses (2000 × 106 sperm/60 mL), and stored at ~16 °C. On day 3 of conservation, sperm were analyzed and used for AI (n = 174). High sperm quality was observed after storage without a significant difference between the groups (p > 0.05). Moreover, no differences were obtained for AI performance (pregnancy and farrowing rates, and litter size; p > 0.05) and offspring health (growth and blood analysis; p > 0.05). Conclusively, the presence of all ejaculate fractions within the seminal doses does not impair the reproductive performance, reporting important economic savings according to the economic model included here.

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Effects of automated collection methods on semen quality and economic efficiency of boar semen production

Cited by 6 publications

References 0 publications

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Should All Fractions of the Boar Ejaculate Be Prepared for Insemination Rather Than Using the Sperm Rich Only?

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