Effects of avocado seed extract in different trisextenders on sperm and oxidative stress indices of vitrified goat spermatozoa

Abstract

Effects of avocado seed extract (ASE) in different tris-extenders on sperm and seminal oxidative stress indices of vitrified spermatozoa of West African Dwarf (WAD) goats during vitrification were assessed. Semen samples were diluted with varying levels (0, 5, 10, 15 and 20 mL/100 mL of diluent) of ASE in tris citric acid (TCA), tris sodium phosphate (TSP) and tris sodium citrate (TSC) extenders while a tris-extender without citric acid, sodium phosphate and sodium citrate served as control. Diluted semen samples were vitrified and preserved. The results revealed higher (P<0.05) progressive sperm motility in TCA extenders at 10%, 15% and 20%, and TSP extender at 20% ASE. Acrosome integrities were higher (P<0.05) in the different tris-extenders at 15% and 20% ASE. TCA, TSP and TSC extenders had higher (P<0.05) membrane integrities at 10%, 15% and 20% ASE. TSP extenders had higher (P<0.05) live sperm at 20% ASE. TCA extenders had higher acrosome reaction while TSP at 20% ASE and TSC at 15% and 20% of ASE had higher sperm capacitation. TCA extender at 20% ASE had reduced malondialdehyde (MDA) concentrations while higher acrosin activities were observed at 20% ASE in TCA extender (P<0.05). The findings indicated that 20% ASE in the tris-extenders improved sperm and oxidative stress indices and the tris-extenders were ranked best in this order: TCA > TSP > TSC.

Ameliorative effect of avocado oil against lufenuron induced testicular damage and infertility in male rats

“…The property of phenolic content to remove oxidative radicals, that is vital for oxidative stress homeostasis, is mainly responsible for their anti‐inflammatory properties (Murakami et al, 2015; Tremocoldi et al, 2018). According to Olamitibo et al (2016), phenolic compounds in avocado seeds can protect spermatozoids in goat sperm from the damaging effects of free radicals during sperm cryopreservation. Avocado pulp extract significantly protected rats' thyroid glands from toxicity caused by chlorpyrifos and carbon tetrachloride (Hamouda et al, 2016; Kassab & El‐Aasr, 2018).…”

"Effects of the Ethanolic Extract of Avocado (Persea americana M.) Seed Powder on Growth Characteristics and Reproductive Performance of Female Cavies (Cavia porcelus L.)"

“…The presence of the above mentioned molecules confers to avocado seeds, many pharmacological properties (estrogenic, antioxidants, anti-inflammatory etc.) [11,30,31]. Due to antioxidant properties of the avocado seed powder, it can be used to fight oxidative stress, thus preventing free radical phenomena, harmful to reproduction and therefore improve production performance in cavies.…”