Effects of Bacillus subtilis Natto NB205 and Its Mutant NBMK308 on Egg Quality in Aging Laying Hens

Liao, Chaoyong; Cui, Jian; Lei, Jiaqi; Guo, Yuming; Zhang, Bingkun

doi:10.3390/life13051109

Cited by 3 publications

(1 citation statement)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Western blotting for differentially expressed proteins was performed according to a previously reported method. − Cells were lysed with RIPA buffer (Thermo Fisher Scientific, Waltham, USA) containing protease and phosphatase inhibitors (Thermo Fisher Scientific, Waltham, USA). Protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA).…”

Section: Methodsmentioning

confidence: 99%

Dual-sgRNA CRISPRa System for Enhanced MK-7 Production and Salmonella Infection Mitigation in Bacillus subtilis natto Applied to Caco-2 Cells

Liao,

Cui,

Gao

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

This study optimized the menaquinone-7 (MK-7) synthetic pathways in Bacillus subtilis (B. subtilis) natto NB205, a strain that originated from natto, to enhance its MK-7 production. Utilizing mutation breeding, we developed NBMK308, a mutant strain that demonstrated a significant 117.23% increase in MK-7 production. A comprehensive transcriptome analysis identified two key genes, ispA and ispE, as being critical in MK-7 synthesis. The dual-sgRNA CRISPRa system was utilized to achieve precise regulation of ispA and ispE in the newly engineered strain, A3E3. This strategic modulation resulted in a significant enhancement of MK-7 production, achieving increases of 20.02% and 201.41% compared to traditional overexpression systems and the original strain NB205, respectively. Furthermore, the fermentation supernatant from A3E3 notably inhibited Salmonella invasion in Caco-2 cells, showcasing its potential for combating such infections. The safety of the dual-sgRNA CRISPRa system was confirmed through cell assays. The utilization of the dual-sgRNA CRISPRa system in this study was crucial for the precise regulation of key genes in MK-7 synthesis, leading to a remarkable increase in production and demonstrating additional therapeutic potential in inhibiting pathogenic infections. This approach effectively combined the advantages of microbial fermentation and biotechnology, addressing health and nutritional challenges.

show abstract

Section: Methodsmentioning

confidence: 99%

Dual-sgRNA CRISPRa System for Enhanced MK-7 Production and Salmonella Infection Mitigation in Bacillus subtilis natto Applied to Caco-2 Cells

Liao,

Cui,

Gao

et al. 2024

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

Advances in probiotics research: mechanisms of action, health benefits, and limitations in applications

Prajapati,

Bisani,

Prajapati

et al. 2023

Syst Microbiol and Biomanuf

View full text Add to dashboard Cite

Meta-analysis of dietary Bacillus spp. on serum biochemical and antioxidant status and egg quality of laying hens

Ogbuewu,

Mabelebele,

Mbajiorgu

2024

Open Agriculture

View full text Add to dashboard Cite

The purpose of the current meta-analysis was to determine the efficacy of Bacillus spp. (BS) feed additive in enhancing serum biochemical indices (total protein and cholesterol) and antioxidant enzymes (total antioxidant capacity [TAC], catalase [CAT], superoxide dismutase [SOD], glutathione peroxidase [GSH-Px], and malondialdehyde [MDA]) status and egg quality (Haugh unit [HU], eggshell thickness [EST], and eggshell strength [ESS]) of laying chickens. Seventeen articles were identified via a systematic search performed on PubMed, Google Scholar, and Scopus, and results were combined using a variance component model. The results indicate that dietary BS supplementation resulted in higher HU (P < 0.001), EST (P < 0.001), and ESS (P < 0.001) values with evidence of heterogeneity. Furthermore, dietary BS increased total protein (P = 0.008) and GSH-Px (P < 0.001) and reduced the concentrations of CAT (P = 0.018), SOD (P = 0.039) and MDA (P < 0.001) in the serum of laying hens. In contrast, dietary BS did not affect serum cholesterol and TAC in laying chickens. Restricted subgroup analyses showed that the studied moderators (i.e., treatment duration, supplementation level, BS, hen’s age, and hen’s strains) influenced the outcomes of the meta-analysis. Additionally, meta-regression revealed that the studied moderators accounted for most of the sources of variations among the 17 studies utilised for the meta-analysis. In conclusion, dietary BS can be utilised as a nutritional strategy to improve egg quality, serum total proteins, and the serum antioxidant status of laying hens.

show abstract

Effects of Bacillus subtilis Natto NB205 and Its Mutant NBMK308 on Egg Quality in Aging Laying Hens

Cited by 3 publications

References 67 publications

Dual-sgRNA CRISPRa System for Enhanced MK-7 Production and Salmonella Infection Mitigation in Bacillus subtilis natto Applied to Caco-2 Cells

Dual-sgRNA CRISPRa System for Enhanced MK-7 Production and Salmonella Infection Mitigation in Bacillus subtilis natto Applied to Caco-2 Cells

Advances in probiotics research: mechanisms of action, health benefits, and limitations in applications

Meta-analysis of dietary Bacillus spp. on serum biochemical and antioxidant status and egg quality of laying hens

Contact Info

Product

Resources

About