Effects of Bacillus subtilis on growth performance and intestinal flora of Penaeus vannamei

Cao, Hongzhen; Chen, Duanduan; Guo, Leifeng; Jv, Rong; Xin, Yunteng; Mo, Wei; Wang, Chen; Li, Pengfei; Wang, Hui

doi:10.1016/j.aqrep.2022.101070

Cited by 10 publications

(8 citation statements)

References 36 publications

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“…The supplementation of Bacillus subtilis in the diet can improve the final weight, weight gain, specific growth rate and condition factor of catfish. A similar result was obtained by (Cao et al, 2022) for Penaeus vannamei supplemented with 0.5% Bacillus subtilis in diet. Mohammadi et al…”

Section: Discussionsupporting

confidence: 86%

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The Effect of Bacillus Subtilis on Growth Rate and Immune Response in Catfish

HAMED,

MOHAMED,

AL SADIK

et al. 2024

Assiut Veterinary Medical Journal

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The study assessed the impact of dietary Bacillus subtilis supplementation on the growth and immune response of catfish. A 2-month feeding trial included control groups (Gr1&Gr2) and Bacillus-treated groups (Gr3&Gr4) with 1X10 10 CFU/kg Bacillus subtilis. Bacillus-treated groups (Gr3 and Gr4) exhibited significant improvements in the last weight, gaining weight, SGR%, and condition factor to the Gr1 and Gr2 groups of controls (Gr1 and Gr2). Additionally, the Bacillus-treated groups exhibited considerably reduced levels of ammonia (NH3) and nitrite (NO2) at the end of the trial. Afterward, (Gr2&Gr4) were infected with Aeromonas hydrophila, and hematological, blood serum parameters, and Aeromonas hydrophila count were assessed. The highest erythrocyte, hemoglobin, and PCV values were observed in the Bacillus-treated group (Gr3). However, RBCs, Hb, and PCV decreased significantly after the pathogen challenge in Gr2 compared to the unchallenged group. A leucogram revealed slight changes in (Gr2, Gr3, and Gr4) compared to (Gr1). The phagocytic activity showed significant enhancement in (Gr3) and significant reduction in (Gr2) compared to other groups. The infected group (Gr2) had increased AST, ALT, urea, creatinine, and TNF-α, along with decreased catalase enzyme, total protein level, albumin, globulin, and lysozyme activity relative to the control group (Gr1). However, Gr4 exhibited significant improvements in all these parameters compared to Gr2. Bacterial load was higher in group (Gr2) but lower in group (Gr4). In summary, this study suggests that adding Bacillus subtilis to the diet may improve the health and growth characteristics of catfish.

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Section: Discussionsupporting

confidence: 86%

“…The groups supplemented with B. subtilis revealed improvement in transaminase activities and kidney function. Likely, the addition of probiotics reduced the activity of transaminase in Penaeus vannamei (Cao et al, 2022). Ghaly et al (2023) suggested that probiotics decrease AST, ALT, urea and creatinine.…”

Section: Bmentioning

confidence: 95%

The Effect of Bacillus Subtilis on Growth Rate and Immune Response in Catfish

HAMED,

MOHAMED,

AL SADIK

et al. 2024

Assiut Veterinary Medical Journal

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“…Rhodobacteraceae possesses complex metabolic pathways and participates in a variety of ecological functions [38,39] 10 Aquaculture Nutrition metabolites [40,41]. Proteobacteria have been identified as being strongly implicated in the degradation of a variety of complex compounds [42]. Verrucomicrobiota members are crucial in the degradation of polysaccharides [43].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study on Effects of Three Butyric Acid-Producing Additives on the Growth Performance, Non-specific Immunity, and Intestinal Microbiota of the Sea Cucumber Apostichopus japonicus

Liu,

Wei,

et al. 2024

Aquaculture Nutrition

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The providers of butyric acid, Clostridium butyricum (CB), sodium butyrate (SB), and tributyrin (TB), have been extensively studied as aquafeed additives in recent years. However, no comparative study has been reported on the probiotic effects of CB, SB, and TB as feed additives on sea cucumber (Apostichopus japonicus). A 63-day feeding trial was performed to assess the effects of dietary live cells of C. butyricum (CB group, the basal diet supplemented with 1% CB), sodium butyrate (SB group, the basal diet supplemented with 1% SB), and tributyrin (TB group, the basal diet supplemented with 1% TB) on the growth, non-specific immunity, and intestinal microbiota of A. japonicus with a basal diet group as the control. Results indicated that all three additives considerably increased A. japonicus growth, with dietary CB having the optimal growth-promoting effect. Of the seven non-specific enzyme parameters measured in coelomocytes of A. japonicus (i.e., the activities of phagocytosis, respiratory burst, superoxide dismutase, alkaline phosphatase, acid phosphatase, catalase, and lysozyme), dietary CB, SB, and TB considerably increased the activities of six, five, and six of them, respectively. The immune genes (Aj-p105, Aj-p50, Aj-rel, and Aj-lys) expression in the mid-intestine tissues of A. japonicus was significantly increased by all three additives. The CB group had the highest expression of all four genes. Additionally, the relative expression of Aj-p105, Aj-p50, and Aj-lys genes was significantly up-regulated in the three additive groups after stimulation with inactivated Vibrio splendidus. Dietary CB enhanced the intestinal microbial diversity and richness in A. japonicus while dietary TB decreased them. Meanwhile, dietary CB, SB, and TB significantly enhanced the abundance of Firmicutes, unclassified_f_Rhodobacteraceae, and Proteobacteria, respectively, while dietary CB and SB reduced the abundance of Vibrio. Dietary CB and SB improved the stability of microbial ecosystem in the intestine of A. japonicus. In contrast, dietary TB appeared to have a negative effect on the stability of intestinal microbial ecosystem. All three additives improved the intestinal microbial functions associated with energy production and immunity regulation pathways, which may contribute directly to growth promotion and non-specific immunity enhancement in A. japonicus. Collectively, in terms of enhancing growth and non-specific immunity, as well as improving intestinal microbiota, dietary live cells of C. butyricum exhibited the most effective effects in A. japonicus.

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“…These parameters are calculated to determine whether the growth of the vannamei shrimp is optimal, since they affect cultivation success (i.e., cost, profit, and loss) [43]. Several studies related to the role of bacteria in the growth performance of vannamei shrimp are shown in Table-1 [16,[44][45][46][47][48][49][50][51][52][53][54][55].…”

Section: Stimulation Of Growth Performance and Survival Ratementioning

confidence: 99%

The role of probiotics in vannamei shrimp aquaculture performance – A review

et al. 2023

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Vannamei shrimp (Litopenaeus vannamei) is an important food commodity of economic benefit due to its high price, low susceptibility to disease, and popularity for consumption. These advantages have led many farmers to cultivate vannamei shrimp. Efforts are underway to improve the aquaculture performance of this species, including the use of probiotics, which are non-pathogenic bacteria that aid in digestion and help fight disease. Probiotics are usually obtained from the intestines of vannamei shrimp or the culture environment. They are low-cost, non-pathogenic, and largely non-toxic source of antibiotics and are able to synthesize various metabolites that have antibacterial functions and applications. Research on probiotic use has primarily been focused on increasing vannamei shrimp aquaculture production. Bacterial species, such as Lactobacillus or Nitrobacter, can be administered orally, by injection, or as a supplement in aquaculture water. Probiotics help to improve survival rate, water quality, immunity, and disease resistance through space competition with disease-causing bacteria, such as Vibrio spp. An increased number of probiotic bacteria suppresses the growth and presence of pathogenic bacteria, which lowers disease susceptibility. In addition, probiotic bacteria also aid digestion by breaking down complex compounds into simpler substances that the body can absorb more easily. This mechanism improves growth performance in terms of weight, length, and feed conversion ratio. This review aimed to provide information regarding contribution of probiotic to improve vannamei shrimp production in aquaculture. Keywords: application, bacteria, farm, microbiome, shrimp.

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Effects of Bacillus subtilis on growth performance and intestinal flora of Penaeus vannamei

Cited by 10 publications

References 36 publications

The Effect of Bacillus Subtilis on Growth Rate and Immune Response in Catfish

The Effect of Bacillus Subtilis on Growth Rate and Immune Response in Catfish

A Comparative Study on Effects of Three Butyric Acid-Producing Additives on the Growth Performance, Non-specific Immunity, and Intestinal Microbiota of the Sea Cucumber Apostichopus japonicus

The role of probiotics in vannamei shrimp aquaculture performance – A review

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