Effects of Bacillus subtilis subsp. on the microbial community and aroma components of flue-cured tobacco leaves based on metagenome analysis

Huang, Shuxian; Liu, Danyang; Chen, Mengyue; Xi, Gaolei; Yang, Pengfei; Jia, Chuanbao; Mao, Duobin

doi:10.1007/s00203-022-03347-1

Cited by 13 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Significant correlations were observed between the changes in Nornicotinoid and Hordanine alkaloid metabolism and the abundance of G racilibacillus, Ruminiclostridium, Fibrella, Conexibacter, and Alkalinema. Gracilibacillus, a genus of Bacillus , has been found to inhibit the alkaloid metabolism of microorganisms ( Huang S. et al, 2022b ). Significant correlations were found between the intermediate products of starch degradation, such as 6-phosphogluconic acid and D-gluconic acid, and the varied abundance of Conexibacter, Fibrella, Valsa, Ruminiclostridiuma, which are known to promote the glycolysis and gluconogenesis pathways ( Silver et al, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Screening, identification, and mechanism analysis of starch-degrading bacteria during curing process in tobacco leaf

Zhang,

Jiang,

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Tobacco, a vital economic crop, had its quality post-curing significantly influenced by starch content. Nonetheless, the existing process parameters during curing were inadequate to satisfy the starch degradation requirements. Microorganisms exhibit inherent advantages in starch degradation, offering significant potential in the tobacco curing process. Our study concentrated on the microbial populations on the surface of tobacco leaves and in the rhizosphere soil. A strain capable of starch degradation, designated as BS3, was successfully isolated and identified as Bacillus subtilis by phylogenetic tree analysis based on 16SrDNA sequence. The application of BS3 on tobacco significantly enhanced enzyme activity and accelerated starch degradation during the curing process. Furthermore, analyses of the metagenome, transcriptome, and metabolome indicated that the BS3 strain facilitated starch degradation by regulating surface microbiota composition and affecting genes related to starch hydrolyzed protein and key metabolites in tobacco leaves. This study offered new strategies for efficiently improving the quality of tobacco leaves.

show abstract

Section: Resultsmentioning

confidence: 99%

Screening, identification, and mechanism analysis of starch-degrading bacteria during curing process in tobacco leaf

Zhang,

Jiang,

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Bacillus belongs to Firmicutes was another dominant genus in the tobacco leaf bacteria. Bacillus is one of the dominant genera in aged tobacco and plays an important role in improving leaf quality during tobacco aging (Ye et al 2017 ; Dai et al 2020 ; Huang et al 2022 ; Wu et al 2023 ). Studies have found that after inoculating Bacillus onto the surface of tobacco, with a decrease in total carbohydrates and a decrease in reducing sugars in tobacco leaves, a pleasant aroma was produced (English et al 1967 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of differences in tobacco leaf microbial communities after redrying in Chinese provinces and from abroad

Zhang,

Xu,

Yang

et al. 2023

AMB Expr

View full text Add to dashboard Cite

Microorganisms play an important role in the tobacco aging process. Before the aging process, raw tobacco leaves must be threshed and redried. In order to explore the differences of microbial community structure of threshed and redried tobacco leaves from different origins at home and abroad, 14 groups of tobacco leaves from 8 different countries were tested by high-throughput DNA sequencing and microbiology analysis. Then, through amplicon sequence variants (ASV) cluster analysis, Venn diagram and species labeling and other microbial diversity analysis, the dominant bacteria and fungi on the surface of threshed and redried tobacco leaves were obtained. The results showed that there were significant differences in the composition of tobacco bacteria and fungi after threshing and redrying from different geographical areas. The relative abundance of Microbacterium and Sphingomonas in domestic tobacco leaves was significantly higher than that of foreign tobacco leaves. The relative abundance of Pseudomonas in foreign tobacco bacterial colonies was significantly higher than that of domestic tobacco leaves. In terms of fungi, the relative abundance of Aspergillus and Alternaria in domestic tobacco leaves was significantly higher than that of foreign tobacco leaves. Septoria, Sampaiozyma, Cladosporium and Phoma account for significantly higher proportions of foreign tobacco leaves. These microorganisms may be indispensable in aging process to form different flavors of tobacco leaves. It provides an important theoretical basis for the further use of microorganisms to promote tobacco leaf aging.

show abstract

“…amyloliquefaciens in the field, where indigenous rhizosphere community shifts were analysed caused by the inoculant‐produced secondary metabolites (Kröber et al, 2014). Similarly, Huang et al (2022) employed metagenomics to investigate the effects of bio‐inoculant B . subtilis subsp.…”

Section: Exploring Bacillus Diversity In Community Settings Using Ngsmentioning

confidence: 99%

“…For instance, metagenome sequencing was applied to track the persistence of inoculated plant protective agent B. amyloliquefaciens in the field, where indigenous rhizosphere community shifts were analysed caused by the inoculant-produced secondary metabolites (Kröber et al, 2014). Similarly, Huang et al (2022) employed metagenomics to investigate the effects of bio-inoculant B. subtilis subsp. H11 on the microbial community structure and the metabolic potential of aged flue-cured tobacco.…”

Section: Exploring Bacillus Diversity In Community Settings Using Ngsmentioning

confidence: 99%

How to identify and quantify the members of the Bacillus genus?

Xu,

Kovács

2024

Environmental Microbiology

View full text Add to dashboard Cite

Members of the Bacillus genus are widely distributed throughout natural environments and have been studied for decades among others for their physiology, genetics, ecological functions, and applications. However, despite its prevalence in nature, the characterization and classification of Bacillus remain challenging due to its complex and ever‐evolving taxonomic framework. This review addresses the current state of the Bacillus taxonomic landscape and summarizes the critical points in the development of Bacillus phylogeny. With a clear view of Bacillus phylogeny as a foundation, we subsequently review the methodologies applied in identifying and quantifying Bacillus, while also discussing their respective advantages and disadvantages.

show abstract

Effects of Bacillus subtilis subsp. on the microbial community and aroma components of flue-cured tobacco leaves based on metagenome analysis

Cited by 13 publications

References 29 publications

Screening, identification, and mechanism analysis of starch-degrading bacteria during curing process in tobacco leaf

Screening, identification, and mechanism analysis of starch-degrading bacteria during curing process in tobacco leaf

Analysis of differences in tobacco leaf microbial communities after redrying in Chinese provinces and from abroad

How to identify and quantify the members of the Bacillus genus?

Contact Info

Product

Resources

About