Effects of Bacillus subtilis var. natto and Saccharomyces cerevisiae fermented liquid feed on growth performance, relative organ weight, intestinal microflora, and organ antioxidant status in Landes geese1

Abstract

The aim of this study was to investigate the effect of Bacillus subtilis var. natto N21 (BAC) and Saccharomyces cerevisiae Y10 (SAC) fermented liquid feed (FLF) during different incubation times on the growth performance, relative organ weight, intestinal microflora, and organ antioxidative status in Landes geese. Two hundred forty male Landes geese (10 wk old) with the BW of 4.163 ± 0.108 kg were selected for a 3-wk trial and randomly allotted to 3 treatments according to their BW (10 replicates/treatment and 8 geese/replicate). The treatments included 1) CON, dry basal feed (corn-soybean basal diet mixed with water) before feeding (2:1 wt/wt), 2) FLF24, 24 h FLF, and 3) FLF48, 48 h FLF. The FLF diet was prepared by storing basal diet with 10(9) cfu/g feed of each BAC and SAC and water (2:1 wt/wt) in a closed tank at 20°C fermented for 24 or 48 h. The BW gain and feed intake of geese fed FLF24 and FLF48 was greater (P < 0.05) than CON treatment. Feeding geese with FLF24 and FLF48 feeds increased (P < 0.05) the relative weight of leg muscle whereas the liver was heavier (P < 0.05) in FLF48 treatment than CON and FLF24 treatments. The FLF24 and FLF48 increased (P < 0.05) the Lactobacillus population and depressed (P < 0.05) Escherichia coli population in small and large intestine. The high-density lipoprotein cholesterol concentration was greatest (P < 0.05) in FLF48 whereas the total cholesterol and low-density lipoprotein cholesterol was less (P < 0.05) in FLF24 and FLF48 treatments than CON. Geese fed FLF48 diet had greater glutathione peroxidase activity and less malondialdehyde content in heart and liver than those fed CON diet. In breast muscle, the superoxide dismutase activity were increased (P < 0.05) by FLF24 and FLF48 treatments than CON diet. In conclusion, the results indicated that feeding geese with BAC and SAC mix FLF can improve growth and feed intake, modulate the intestine ecology, and decrease the blood cholesterol concentrations; it also can improve the antioxidative status of organs and breast muscle.

Yeast fermented additive enhances broiler growth

“…Further decrease in pH of the fermented substrate was normally caused by the productions of organic acids (Canibe and Jensen, 2003;Canibe et al, 2006), whose levels were significantly high (56 g kg −1 ) in this study. Overall, the results of fermentation parameters in this SSF process were similar to the results obtained previously (Yasar et al, 2016;Yasar and Gok, 2014;Chen et al, 2013).…”

“…Furthermore, in our study, there were sporadic changes in heart and liver weights affected by the dietary treatments. Similar changes in digestive organ weights were previously reported with the diet fermented with yeast inoculant at 10 6 cfu kg −1 (Chen et al, 2013, Hossain et al, 2012Chen et al, 2009). In our study, the changes in digestive organ weights were similarly reflected on the changes in the lengths of entire digestive tract, foregut, and small intestine ( Table 5).…”

“…Furthermore, the fermentation process in this study was done with the help of value-adding co-products, i.e., whey, pomaces of tomato and citrus, and rosemary, which could have added new functionalities to the final products. The fermented feed materials produced from this SSF process were found with high efficacies in broilers due to organic acids, flavoring agents, endogenous enzymes, probiotics, and antioxidants from these co-products (Yasar et al, 2016;Yasar and Gok 2014;Chen et al, 2013;Dhillon et al, 2012, Canibe et al, 2006Canibe and Jensen, 2003).…”

FEEDING EFFECT OF INULIN DERIVED FROM DAHLIA TUBER COMBINED WITH Lactobacillus sp. ON MEAT PROTEIN MASS OF CROSSBRED KAMPONG CHICKEN

“…The reduced pathogenic bacteria population can be assumed to have impact on the lightening body defence via phagocytocis load (Abbas and Litshman, 2009;Arief et al, 2010), and this mechanism in the present study was indicated by the higher value of antioxidant activity ( Table 2). As it have been reported by Susanti et al (2012) and Chen et al (2013) that during the process of phagocytosis, phagocytic receptors that bind to bacterial pathogens sends a signal to activate the reactive oxygen species (ROS) such as superoxide anion-free radicals and H 2 O 2 which can damage cells of pathogenic bacteria.…”

Effects of &lt;i&gt;Bacillus subtilis&lt;/i&gt; KN-42 on Growth Performance, Diarrhea and Faecal Bacterial Flora of Weaned Piglets

“…Previous studies reported that the addition of B. subtilis var. natto improved the growth and feed intake of geese (Chen et al, 2013), and the diets supplemented with 10 8 CFU/kg feed of B. subtilis improved the ADG of broilers (Zhang et al, 2012). Other researchers also reported that the supplementation of B. subtilis to diet improved the growth performance of pigs (Alexopoulos et al, 2004; Wang et al, 2011; Lee et al, 2014).…”