Effects of Basic Fibroblast Growth Factor on Irradiated Porcine Skin Flaps

Chang, Henry S.; Hom, David B.; Agarwal, Ravi; Pernell, Kerri J.; Manivel, J. Carlos; Song, Chang W.

doi:10.1001/archotol.124.3.307

Cited by 16 publications

(16 citation statements)

References 31 publications

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“…33 Chang et al, 22 using a porcine model, found a 62% increase in vascularity in irradiated flaps treated with bFGF. Previous studies that used irradiated animal models in conjunction with FGFs have shown mixed results in irradiated conditions.…”

Section: Discussionmentioning

confidence: 98%

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Vascular and Epidermal Effects of Fibroblast Growth Factor on Irradiated and Nonirradiated Skin Flaps

Hom

Simplot

Manivel

et al. 2000

Ann Otol Rhinol Laryngol

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Head and neck cancer surgeons are often faced with the challenge of managing previously irradiated soft tissue that has poor vascularity and slower epithelialization. This study investigates the effect of supplemental basic fibroblast growth factor (bFGF) on flap vascularity, tissue oxygenation, and epidermal regeneration in nonirradiated (n = 40) and irradiated porcine skin flaps (n = 40). Supplemental bFGF increased vascularity in nonirradiated flaps by 80% (p = .005), with a trend to a higher tissue oxygen level by day 14. The irradiated bFGF-treated flaps did not show increased vascularity or higher tissue oxygen levels 2 weeks after surgery. However, in both irradiated and nonirradiated groups, epidermal regeneration increased by greater than 70% with supplemental bFGF (p < .002). The results of this study suggest that supplemental bFGF can increase tissue vascularity in nonirradiated tissues and epidermal regeneration in both nonirradiated and irradiated conditions.

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Section: Discussionmentioning

confidence: 98%

“…22 Treated and control flaps were then harvested from the side of the pig by means of the previous incision lines. On postoperative day 14, after completion of tissue oximetry measurements, each animal was sedated by means of the regimen described previously, and porcine heparin (1 mg/kg) was administered by intravenous injection.…”

Section: Methodsmentioning

confidence: 99%

Vascular and Epidermal Effects of Fibroblast Growth Factor on Irradiated and Nonirradiated Skin Flaps

Hom

Simplot

Manivel

et al. 2000

Ann Otol Rhinol Laryngol

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“…Clinically this can have a signi cant impact, since the induction and inhibition of angiogenesis have an implementation in many diseases [43][44][45][46][47]. The therapeutic angiogenic process can be in uenced by direct stimulation with angiogenic growth factors or via growth factors that are known to stimulate the release of angiogenic factors from cells.…”

Section: Original Researchmentioning

confidence: 99%

A Potential Role for Mast Cells in the of bFGF From Normal Myocytes During Angiogenesis In Vivo

Walgenbach

Gorospe

Gratas

et al. 2002

Journal of Investigative Surgery

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Basic fibroblast growth factor (bFGF) is a potent angiogenic factor produced by cells of mesodermal and neuroectodermal origin. Despite numerous advances, the precise mechanism of bFGF release from cells still remains unknown. Upon release from cells, the protein is stored and protected in the extracellular matrix by binding to heparan sulfate proteoglycans. A number of reports suggest that degrading enzymes secreted by mast cells may play a role in the release of bFGF from connective tissue stores. Additionally, mast cells are believed to play a role in the formation of new blood vessels. In this report, we studied the events involved in neovascularization using a well-characterized model of angiogenesis in rabbits where neovascularization is induced by transfer of a well-perfused rectus abdominis muscle flap to an ischemic limb. Using this model, we demonstrate that bFGF expression is induced in normal myofibers and bFGF is released in the wound fluid at the ischemic/nonischemic interface. The highest concentrations of bFGF were detected on days 14 and 21 postoperation. We also show that the number of mast cells and their degranulation correlate with the release of bFGF from adjacent muscle tissue and the appearance of the growth factor in the wound fluid. There appears to exist a temporal correlation between number of mast cells, their degranulation, and the release of bFGF during angiogenesis in vivo.

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“…Thus, supplemental bFGF may be important for irradiated postsurgical soft tissue healing. It has been shown that the use of bFGF prior to bone grafting improved vascularity in the irradiated soft tissue bed, which reduced the risk of bone graft failure [29], and that intravenous bFGF improved random skin flap viability and increased skin flap tolerance to irradiation [27,30].…”

Section: Evaluation Of the Hypothesis/ideamentioning

confidence: 99%

Transdermal bFGF delivery using low-frequency sonophoresis: An innovative potential therapy for osteoradionecrosis of jaws

Zhou

Wang

Lang

et al. 2015

Journal of Medical Hypotheses and Ideas

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Osteoradionecrosis of jaws (ORNJ) is a serious complication of radiotherapy for patients with head and neck cancer. As of yet, no universally accepted treatment exists for this chronic pathologic condition. It has been shown that ultrasound is an effective, noninvasive adjunctive therapy in ORNJ, as ultrasound can result in the increase of angiogenesis and bone production, which are essential for ORNJ healing. Recently, low-frequency ultrasound has been demonstrated to enhance the transdermal delivery of macromolecules and hydrophilic drugs (low-frequency sonophoresis, LFS). As a biological macromolecule, basic fibroblast growth factor (bFGF) also has potential osteoinductive and angiogenic properties. Herein, we present a hypothesis that LFS-mediated transdermal bFGF delivery is capable of improving the healing of ORNJ and will be a new effective adjunctive therapy to surgery. This treatment combines low-frequency ultrasound with bFGF to respectively promote vascularly compromised bone and soft tissue wound healing, and is expected to be more effective than ultrasound therapy alone.

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Effects of Basic Fibroblast Growth Factor on Irradiated Porcine Skin Flaps

Abstract: This study provides evidence that supplemental bFGF can increase vascularity to skin flaps in previously irradiated porcine skin tissue. Histologically, radiation did not prevent the angiogenic effect of bFGF.

Cited by 16 publications

References 31 publications

Vascular and Epidermal Effects of Fibroblast Growth Factor on Irradiated and Nonirradiated Skin Flaps

Vascular and Epidermal Effects of Fibroblast Growth Factor on Irradiated and Nonirradiated Skin Flaps

A Potential Role for Mast Cells in the of bFGF From Normal Myocytes During Angiogenesis In Vivo

Transdermal bFGF delivery using low-frequency sonophoresis: An innovative potential therapy for osteoradionecrosis of jaws

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