Effects of bee density and sublethal imidacloprid exposure on cluster temperatures of caged honey bees

Meikle, William G.; Adamczyk, John J.; Weiss, Milagra; Gregorc, Aleš

doi:10.1007/s13592-018-0585-z

Cited by 17 publications

(10 citation statements)

References 33 publications

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“…Poor forage in AZ 2017 likely affected residue concentrations in the stored honey. Clothianidin was stable in honey for several months after the end of treatment, as has been found with imidacloprid 27 , and clothianidin residues in stored honey were much higher in AZ 2017 than the following year, suggesting less dilution from alternative nectar sources and a higher dose per bee.…”

Section: Discussionsupporting

confidence: 60%

“…Similarly, a field study involving “mini-colonies” challenged with both Nosema and clothianidin found no effect of clothianidin treatment on mortality or flight activity, and while the lifespans of Nosema infected bees were reduced compared to non-infected bees a combination of pesticide and pathogen did not reveal any synergistic effect 26 . Experiments with imidacloprid have also had mixed results with respect to colony growth and thermoregulation 19 , 20 , 27 .…”

Section: Introductionmentioning

confidence: 99%

“…Pesticide levels would be elevated in the stored honey so essentially all the bees in treated colonies would have had some degree of exposure by the end of the experiment. Treatment concentrations were based both on those used in previous studies on the exposure of bees to neonicotinoids 20 , 27 and on values reported from large-scale surveys 41 . Acute oral LD 50 of clothianidin to honey bees is about 4 ng per bee (Pesticide Properties Database: https://sitem.herts.ac.uk/aeru/ppdb/en/atoz_insect.htm ), or a single dose of about 200 mg of the 20-ppb syrup, which exceeds the maximum estimated daily intake for even high-consumption nectar foragers 42 .…”

Section: Introductionmentioning

confidence: 99%

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Sublethal concentrations of clothianidin affect honey bee colony growth and hive CO2 concentration

Meikle

Adamczyk²,

Weiss

et al. 2021

Sci Rep

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The effects of agricultural pesticide exposure upon honey bee colonies is of increasing interest to beekeepers and researchers, and the impact of neonicotinoid pesticides in particular has come under intense scrutiny. To explore potential colony-level effects of a neonicotinoid pesticide at field-relevant concentrations, honey bee colonies were fed 5- and 20-ppb concentrations of clothianidin in sugar syrup while control colonies were fed unadulterated syrup. Two experiments were conducted in successive years at the same site in southern Arizona, and one in the high rainfall environment of Mississippi. Across all three experiments, adult bee masses were about 21% lower among colonies fed 20-ppb clothianidin than the untreated control group, but no effects of treatment on brood production were observed. Average daily hive weight losses per day in the 5-ppb clothianidin colonies were about 39% lower post-treatment than in the 20-ppb clothianidin colonies, indicating lower consumption and/or better foraging, but the dry weights of newly-emerged adult bees were on average 6–7% lower in the 5-ppb group compared to the other groups, suggesting a nutritional problem in the 5-ppb group. Internal hive CO2 concentration was higher on average in colonies fed 20-ppb clothianidin, which could have resulted from greater CO2 production and/or reduced ventilating activity. Hive temperature average and daily variability were not affected by clothianidin exposure but did differ significantly among trials. Clothianidin was found to be, like imidacloprid, highly stable in honey in the hive environment over several months.

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Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sublethal concentrations of clothianidin affect honey bee colony growth and hive CO2 concentration

Meikle

Adamczyk²,

Weiss

et al. 2021

Sci Rep

Self Cite

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“…Internal hive temperature over time has been linked to changes in colony size and phenology [26, 31]. Sublethal doses of pesticides have been associated with temperature regulation by bees in both field [28] and cage studies [37]. In this study, internal hive temperature was significantly more variable in the 200 ppb treatment group during December and January (months with the lowest ambient temperatures).…”

Section: Discussionmentioning

confidence: 65%

Exposure to sublethal concentrations of methoxyfenozide disrupts honey bee colony activity and thermoregulation

et al. 2019

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Methoxyfenozide is an insect growth regulator (IGR) commonly used in agriculture to simultaneously control pests and preserve beneficial insect populations; however, its impact on honey bees in not fully understood. We conducted field and laboratory experiments to investigate bee health in response to field-relevant concentrations of this pesticide. Significant effects were observed in honey bee colony flight activity and thermoregulation after being exposed over 9 weeks to supplemental protein patty containing methoxyfenozide. Compared to bee colonies in the control group, colonies fed pollen patty with 200 ppb methoxyfenozide (as measured by residue analysis) had: 1) a significantly reduced rate of weight loss due to forager departure in the morning; and 2) higher temperature variability during the winter. Colonies in the 100 ppb (as measured by residue analysis) treatment group had values between the 200 ppb group and control for both response variables. The dusk break point, which is the time associated with the end of forager return, differed among all treatment groups but may have been confounded with direction the hives were facing. Bee colony metrics of adult bee mass and brood surface area, and measurements of bee head weight, newly-emerged bee weight, and hypopharyngeal gland size were not significantly affected by methoxyfenozide exposure, suggesting that there may be significant effects on honey bee colony behavior and health in the field that are difficult to detect using standard methods for assessing bee colonies and individuals. The second experiment was continued into the following spring, using the same treatment groups as in the fall. Fewer differences were observed among groups in the spring than the fall, possibly because of abundant spring forage and consequent reduced treatment patty consumption. Residue analyses showed that: 1) observed methoxyfenozide concentrations in treatment patty were about 18–60% lower than the calculated concentrations; 2) no residues were observed in wax in any treatment; and 3) methoxyfenozide was detected in bee bread only in the 200 ppb treatment group, at about 1–2.5% of the observed patty concentration.

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“…Thus, if lethal acute effects can be rapidly noticed by the rapid decrease of the population or mortalities in front of the hive, sub-lethal effects can be difficult to observe, as colonies generally have problems of development or slow mortality for longer periods (autumn, winter, and early spring depopulation), inducing a variety of behavioral dysfunctions. Many of these dysfunctions affect orientation, memory, communication [46][47][48], foraging and flight [49,50], the olfactory sense [51], the glandular system and respiratory rhythm [52,53], reproduction [54,55], global temperature and metabolism [56,57], sensitivity to diseases, and immunity reduction [58][59][60][61][62][63][64].…”

Section: Discussionmentioning

confidence: 99%

Monitoring the Field-Realistic Exposure of Honeybee Colonies to Neonicotinoids by An Integrative Approach: A Case Study in Romania

et al. 2020

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Honeybees (Apis mellifera L.) are excellent biosensors that can be managed to collect valuable information about environmental contamination. The main objective of the present study was to design and apply an integrative protocol to monitor honeybee colony activity and sample collection by using electronic technologies combined with classical methods in order to evaluate the exposure of honeybees to the neonicotinoids that are used in melliferous intensive crops. The monitored honeybee colonies were especially prepared and equipped to maximize their chances to collect representative samples in order to express, as well as possible, the pesticide residues that existed in the targeted crops. The samples of honey, pollen and honeybees were collected, preserved and prepared to fulfill the required quality and quantity criteria of the accredited laboratories. In total, a set of fifty samples was collected from fields, located in different areas of intensive agriculture in Romania, and was analyzed for five neonicotinoids. The obtained results show that 48% of the total analyzed samples (n = 50) contained one or more detected or quantified neonicotinoid residues. The main conclusion is that the proposed approach for sample collection and preparation could improve the evaluation methodologies for analyzing honeybees’ exposure to pesticides.

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Effects of bee density and sublethal imidacloprid exposure on cluster temperatures of caged honey bees

Cited by 17 publications

References 33 publications

Sublethal concentrations of clothianidin affect honey bee colony growth and hive CO2 concentration

Sublethal concentrations of clothianidin affect honey bee colony growth and hive CO2 concentration

Exposure to sublethal concentrations of methoxyfenozide disrupts honey bee colony activity and thermoregulation

Monitoring the Field-Realistic Exposure of Honeybee Colonies to Neonicotinoids by An Integrative Approach: A Case Study in Romania

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