Effects of benzyl isothiocyanate on the reproduction of Meloidogyne incognita on Glycine max and Capsicum annuum

Masler, Edward P.; Zasada, Inga A.; Sardanelli, Sandra; Rogers, Stephen T.; Halbrendt, J. M.

doi:10.1163/138855409x12595802046375

Cited by 7 publications

(4 citation statements)

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“…Exposure of Meloidogyne incognita J2 to a sublethal dose of benzyl isothiocyanate had a significant impact on subsequent infectivity and reproduction on plant hosts. 24 A sublethal dose of diflubenzuron resulted in a lower net reproductive rate of Culex quinquefasciatus, whereas a sublethal dose of azadirachtin did not. 25 It is impossible to speculate on the long-term consequences of exposure to sublethal doses of nitrile 3, thioamide 4, and isothiocyanate 2 against M. hapla, P. irregulare, and V. dahliae in this study.…”

Section: ■ Discussionmentioning

confidence: 95%

“…However, there may also be fitness costs that are not favorable to microorganisms. Exposure of Meloidogyne incognita J2 to a sublethal dose of benzyl isothiocyanate had a significant impact on subsequent infectivity and reproduction on plant hosts . A sublethal dose of diflubenzuron resulted in a lower net reproductive rate of Culex quinquefasciatus, whereas a sublethal dose of azadirachtin did not .…”

Section: Discussionmentioning

confidence: 98%

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Activity of Meadowfoam (Limnanthes alba) Seed Meal Glucolimnanthin Degradation Products against Soilborne Pathogens

Zasada

Weiland

Reed

et al. 2011

J. Agric. Food Chem.

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Meadowfoam (Limnanthes alba L.) is a herbaceous winter-spring annual grown as a commercial oil seed crop. The meal remaining after oil extraction from the seed contains up to 4% of the glucosinolate, glucolimnanthin. Degradation of glucolimnanthin yields toxic breakdown products and therefore the meal may have potential in the management of soil-borne pathogens. To maximize the pest suppressive potential of meadowfoam seed meal, it would be beneficial to know the toxicity of individual glucolimnanthin degradation products against specific soil-borne pathogens. Meloidogyne hapla second-stage juveniles (J2) and Pythium irregulare and Verticillium dahliae mycelial cultures were exposed to glucolimnanthin as well as its degradation products. Glucolimnanthin and its degradation product, 2-(3-methoxyphenyl)acetamide, were not toxic to any of the soil-borne pathogens at concentrations up to 1.0 mg/mL. Two other degradation products, 2-(3-methoxymethyl)ethanethioamide and 3-methoxyphenylacetonitrile, were toxic to M. hapla and P. irregulare but not V. dahliae. The predominant enzyme degradation product, 3-methoxybenzyl isothiocyanate was the most toxic compound against all of the soil-borne pathogens with M. hapla being the most sensitive with EC50 values (0.0025 ± 0.0001 to 0.0027 ± 0.0001 mg/mL) 20 to 40 times lower than estimated EC50 mortality values generated for P. irregulare and V. dahliae (0.05 and 0.1 mg/mL, respectively). The potential exists to manipulate meadowfoam seed meal to promote the production of specific degradation products. The conversion of glucolimnanthin into its corresponding isothiocyanate should optimize the biopesticidal properties of meadowfoam seed meal against M. hapla, P. irregulare, and V. dahliae.

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Section: ■ Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Activity of Meadowfoam (Limnanthes alba) Seed Meal Glucolimnanthin Degradation Products against Soilborne Pathogens

Zasada

Weiland

Reed

et al. 2011

J. Agric. Food Chem.

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“…The infectivity of treated (0.01 mM) M. incognita juveniles on soybean ( Glycine max ) was significantly reduced, and egg production was almost eliminated by 0.03 mM benzyl isothiocyanate ( Zasada et al., 2009 ). Reproduction and infectivity of M. incognita were suppressed when juveniles (J2) were exposed to 0.03 mM benzyl isothiocyanate for 2 h prior to inoculation with either C. annuum or G. max ( Masler et al., 2010 ). However, the mode of nematicidal action of these isothiocyanates remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Valorization of the essential oil from Drypetes gossweileri S. Moore (Putranjivaceae): in vitro, in vivo, and in silico nematicidal activity

Mbula,

Andres,

Kitete

et al. 2023

Front. Plant Sci.

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The chemical composition, insect antifeedant, in vtro/in vivo nematicidal activity, phytotoxicity, and in silico nematicidal activity of the essential oil (EO) of the African medicinal plant Drypetes gossweileri were studied. Chemical analysis using GC/MS indicated that benzyl isothiocyanate (96.23%) was the major compound, followed by benzyl cyanide (1.38%). The biocidal effects of this oil were tested against insect pests and root-knot nematodes. All the insect species tested were significantly affected by the oil according to their feeding adaptations (Spodoptera littoralis and Myzus persicae were less affected than Rhopalosiphum padi) with efficient doses (EC50) of 29.4 8.3 μg/cm2, 14.744 8.3 μg/cm2, and 8.3 μg/cm2, respectively. The oil was highly effective against juveniles J2 of the nematode Meloidogyne javanica, with LC50–LC90 values of 0.007 mg/mL–0.0113 mg/mL. D. gossweileri EO at minimum lethal concentrations (MLC) and below strongly inhibited egg hatching in vitro, whereas soil treatment caused a strong suppression of nematode population, infection frequency, and multiplication rate. The EO inhibited ryegrass (Lolium perenne) germination at 0.4 mg/mL, while at 0.1 mg/mL, its effects on germination, root and leaf growth were moderate (32.4%, 8.4%, and 18.3%, respectively). The tomato (Solanum lycopersicum) germination was not affected by the EO, but the root growth was reduced (56% at 0.1 mg/mL) at a dose 10 times higher than the LD50 calculated for M. javanica J2 mortality. Molecular docking of the nematicidal effects of the oil using PyRx revealed a strong interaction between potassium chloride transporting KCC3 (PDB ID: 7D90) and benzyl cyanide at a distance of 2.20 A° with GLN C:350, followed by benzyl isothiocyanate at a distance of 2.78 A° with ARG B:294. The in vivo nematicidal effects of D. gossweileri EO on M. javanica penetration and reproduction in tomato roots further support the potential of this EO as a nematicidal agent with insect antifeedant effects, which could be used by local farmers for crop protection.

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“…For example, SCNkilled or suppressed germination of a number of plants, including the crop plants carrot, corn (Zea mays), pea (Pisum sativum), and wheat (Triticum aestivum) (Hansson et al, 2008;Harvey, 1931). Ionic thiocyanate and other ITCs are also active against nematodes (Buskov et al, 2002;Donkin et al, 1995;Lazzeri et al, 1993Lazzeri et al, , 2004Masler et al, 2010;Zasada and Ferris, 2003). Addition of mustard seed meals can also affect other organisms and suppress plant diseases by altering available nitrogen and bacterial communities in the soil (Hollister et al, 2013;Reardon et al, 2013;Weerakoon et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Mustard Seed Meal for Management of Root-knot Nematode and Weeds in Tomato Production

Meyer

Zasada

Rupprecht

et al. 2015

hortte

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Mustard seed meals of indian mustard [InM (Brassica juncea)] and yellow mustard [YeM (Sinapis alba)], alone and combined, were tested for effects on tomato (Solanum lycopersicum) plants and for suppression of southern root-knot nematode [RKN (Meloidogyne incognita)] and weed populations. In the greenhouse, with all seed meal treatments applied at 0.25% total w/w soil, low tomato plant stands (up to 60% dying/dead) resulted from amendment with 3 YeM:1 InM, 1 YeM:1 InM, and YeM, applied right before transplant. Compared with untreated controls, low numbers of RKN eggs per gram root were consistently recorded from amendment with 3 YeM:1 InM. In a 2012 field study, incorporation of 1 YeM:1 InM (1700 lb/acre) resulted in lower tomato root biomass than fertilizer application (504 lb/acre), YeM or InM (each 1700 lb/acre). All treatments were applied with added fertilizer to achieve 100–102 lb/acre nitrogen, 7.4 lb/acre phosphorus, 74.7 lb/acre potassium, 6.0 lb/acre sulfur, and 1.0 lb/acre boron. The lowest numbers of RKN eggs per gram root (harvest 2012) were collected from plots amended with InM (1700 lb/acre), YeM (850 lb/acre), and 3 YeM:1 InM (1700 lb/acre), but the numbers were not significantly different from fertilizer only (504 lb/acre) controls. Highest and lowest tomato yields (numbers of fruit) in 2012 were recorded from YeM (850 lb/acre) and 3 YeM:1 InM (1700 lb/acre) amendments, respectively. In 2013, there were no significant differences among treatments in eggs per gram root or in tomato yields. No mustard seed meal treatment affected weed populations. At the tested rates, YeM seed meal showed potential for use in tomato beds but results were inconsistent between years.

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Effects of benzyl isothiocyanate on the reproduction of Meloidogyne incognita on Glycine max and Capsicum annuum

Cited by 7 publications

References 18 publications

Activity of Meadowfoam (Limnanthes alba) Seed Meal Glucolimnanthin Degradation Products against Soilborne Pathogens

Activity of Meadowfoam (Limnanthes alba) Seed Meal Glucolimnanthin Degradation Products against Soilborne Pathogens

Valorization of the essential oil from Drypetes gossweileri S. Moore (Putranjivaceae): in vitro, in vivo, and in silico nematicidal activity

Mustard Seed Meal for Management of Root-knot Nematode and Weeds in Tomato Production

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