Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice

Li, Guanghui; Zhang, Yaping; Tang, Jinliang; Chen, Zhengtang; Hu, Yun; Hong, Wei; Li, Dezhi; Hao, Ping; Wang, Donglin

doi:10.1016/j.ijrobp.2010.02.062

Cited by 17 publications

(10 citation statements)

References 39 publications

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“…In this current study, we demonstrated that the levels of the pro-inflammatory cytokines IL-1 β and TNF- α significantly increased in plasma in the early phase following radiotherapy, but no significant changes were observed in the late phase. In a previous study performed in mice, plasma TNF- α and IL-10 levels were shown to be elevated 6 h after abdominal radiation [ 24 ]. Collectively, in prostate cancer patients an immediate elevation of IL-1 β and IL-6 in plasma following irradiation has also been demonstrated [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Investigation into the role of the cholinergic system in radiation-induced damage in the rat liver and ileum

Özyurt

Ozden

Çevik

et al. 2014

Journal of Radiation Research

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It has been previously shown that acetylcholine (ACh) may affect pro-inflammatory and anti-inflammatory cytokines. The role of the cholinergic system in radiation-induced inflammatory responses and tissue damage remains unclear. Therefore, the present study was designed to determine the radio-protective properties of the cholinergic system in the ileum and the liver of rats. Rats were exposed to 8-Gy single-fraction whole-abdominal irradiation and were then decapitated at either 36 h or 10 d post-irradiation. The rats were treated either with intraperitoneal physiological saline (1 ml/kg), physostigmine (80 µg/kg) or atropine (50 μg/kg) twice daily for 36 h or 10 d. Cardiac blood samples and liver and ileal tissues were obtained in which TNF-α, IL-1β and IL-10 levels were assayed using ELISA. In the liver and ileal homogenates, caspase-3 immunoblots were performed and myeloperoxidase (MPO) activity was analyzed. Plasma levels of IL-1β and TNF-α increased significantly following radiation (P < 0.01 and P < 0.001, respectively) as compared with non-irradiated controls, and physostigmine treatment prevented the increase in the pro-inflammatory cytokines (P < 0.01 and P < 0.001, respectively). Plasma IL-10 levels were not found to be significantly changed following radiation, whereas physostigmine augmented IL-10 levels during the late phase (P < 0.01). In the liver and ileum homogenates, IL-1β and TNF-α levels were also elevated following radiation, and this effect was inhibited by physostigmine treatment but not by atropine. Similarly, physostigmine also reversed the changes in MPO activity and in the caspase-3 levels in the liver and ileum. Histological examination revealed related changes. Physostigmine experiments suggested that ACh has a radio-protective effect not involving the muscarinic receptors.

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Section: Discussionmentioning

confidence: 99%

Investigation into the role of the cholinergic system in radiation-induced damage in the rat liver and ileum

Özyurt

Ozden

Çevik

et al. 2014

Journal of Radiation Research

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“…Berberine has also been shown to reduce the incidence and severity of acute intestinal symptoms in patients receiving pelvic radiation [313]. In vivo studies in mice showed the protective effects of berberine against radiation-induced intestinal injury by decreasing inflammation markers, lipid peroxidation, and mucosal injury in the intestinal tissue [314,315]. Moreover, berberine decreased markers of endothelial dysfunction and reduced the incidence of lung injury induced by radiation therapy in patients with non-small cell lung cancer [316].…”

Section: Protective Effects Of Cyp1b1 Inhibitors Against Radiation-inmentioning

confidence: 99%

CYP1B1 as a therapeutic target in cardio-oncology

2020

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Cardiovascular complications have been frequently reported in cancer patients and survivors, mainly because of various cardiotoxic cancer treatments. Despite the known cardiovascular toxic effects of these treatments, they are still clinically used because of their effectiveness as anti-cancer agents. In this review, we discuss the growing body of evidence suggesting that inhibition of the cytochrome P450 1B1 enzyme (CYP1B1) can be a promising therapeutic strategy that has the potential to prevent cancer treatment-induced cardiovascular complications without reducing their anti-cancer effects. CYP1B1 is an extrahepatic enzyme that is expressed in cardiovascular tissues and overexpressed in different types of cancers. A growing body of evidence is demonstrating a detrimental role of CYP1B1 in both cardiovascular diseases and cancer, via perturbed metabolism of endogenous compounds, production of carcinogenic metabolites, DNA adduct formation, and generation of reactive oxygen species (ROS). Several chemotherapeutic agents have been shown to induce CYP1B1 in cardiovascular and cancer cells, possibly via activating the Aryl hydrocarbon Receptor (AhR), ROS generation, and inflammatory cytokines. Induction of CYP1B1 is detrimental in many ways. First, it can induce or exacerbate cancer treatment-induced cardiovascular complications. Second, it may lead to significant chemo/radio-resistance, undermining both the safety and effectiveness of cancer treatments. Therefore, numerous preclinical studies demonstrate that inhibition of CYP1B1 protects against chemotherapy-induced cardiotoxicity and prevents chemo- and radio-resistance. Most of these studies have utilized phytochemicals to inhibit CYP1B1. Since phytochemicals have multiple targets, future studies are needed to discern the specific contribution of CYP1B1 to the cardioprotective and chemo/radio-sensitizing effects of these phytochemicals.

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“…In addition to the synergistic effects against cancer, berberine also has protective effects against radiation-induced injury. It has been reported that berberine significantly reduces the incidence of radiationinduced lung injury and intestinal injury (21,22). Berberine may be a useful therapeutic agent for breast cancer therapy.…”

Section: Introductionmentioning

confidence: 99%

Radiosensitization effects of berberine on human breast cancer cells

Wang

Yang

2012

International Journal of Molecular Medicine

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Berberine, an isoquinoline derivative alkaloid, has recently been shown to have antitumor activity. The present study aimed to investigate the effects of the concomitant administration of berberine and radiation on breast cancer. The effects of berberine on the radiosensitivity of MCF-7 and MDA-MB-468 cells were evaluated by using cell clonogenic assays. Cells pre-treated with berberine or dimethyl sulfoxide (DMSO) for 24 h were irradiated using a Faxitron Cabinet X-ray System to deliver the indicated doses (0, 1, 2, 3 and 4 Gy). Changes in cell cycle distribution were determined by flow cytometry. γ-H2AX foci were detected by immunofluorescence staining. The levels of Ku70, Ku86 and RAD51 proteins were evaluated by western blot analysis. We observed that berberine increased the MCF-7 and MDA-MB-468 cell radiosensitivity with cell clonogenic assays. the radiation-induced G2/M cell cycle delay was reduced in the MCF-7 cells pre-teated with berberine. Berberine pre-treatment prolonged the persistence of DNA double-strand breaks in the MCF-7 cell line. In comparison with the control cells, the protein levels of RAD51 were decreased in the MCF-7 and MDA-MB-468 cells treated with berberine, and in the cells pre-treated with 15 µM berberine for 24 h, the level of RAD51 protein decreased significantly at the indicated time-points (0, 2, 6 and 24 h) following X-ray exposure. In conclusion, berberine sensitizes human breast cancer cells to ionizing radiation by inducing cell cycle arrest and the downregulation of the homologous recombination repair protein, RAD51. Berberine may be a promising radiosensitizer for the treatment of breast cancer.

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Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice

Cited by 17 publications

References 39 publications

Investigation into the role of the cholinergic system in radiation-induced damage in the rat liver and ileum

Investigation into the role of the cholinergic system in radiation-induced damage in the rat liver and ileum

CYP1B1 as a therapeutic target in cardio-oncology

Radiosensitization effects of berberine on human breast cancer cells

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