Effects of bevacizumab loaded PEG-PCL-PEG hydrogel intracameral application on intraocular pressure after glaucoma filtration surgery

Abstract

PEG-PCL-PEG (PECE) hydrogel for intracameral injection as a sustained delivery system can get a stable release of the medication and achieve an effective local concentration. The injectable PECE hydrogel is thermosensitive nano-material which is flowing sol at low temperature and can shift to nonflowing gel at body temperature. This study evaluated the intracameral injection of bevacizumab combined with a PECE hydrogel drug release system on postoperative scarring and bleb survival after experimental glaucoma filtration surgery. The best result was achieved in the bevacizumab loaded PECE hydrogels group, which presented the lowest IOP values after surgery. And the blebs were significantly more persistent in this group. Histology, Massion trichrome staining and immunohistochemistry further demonstrated that glaucoma filtration surgery in combination with bevacizumab loaded PECE hydrogel resulted in good bleb survival due to scar formation inhibition. In conclusions, this study demonstrated that bevacizumab-loaded PECE hydrogel for intracameral injection as a sustained delivery system provide a great opportunity to increase the therapeutic efficacy of glaucoma filtration surgery.

Ditelluride-Bridged PEG-PCL Copolymer as Folic Acid-Targeted and Redox-Responsive Nanoparticles for Enhanced Cancer Therapy

“…Poly(ethylene glycol) monomethylether (PEG, Mw = 5000) and 11-bromoundecanol were obtained from Sigma-Aldrich. The block copolymers of poly(ethylene glycol) monomethylether and poly(ε-caprolactone) (PEG-b-PCL) was synthesized as previously reported and the average molecular weights of PCL was 5930 (Han et al, 2015). FA-PEG-b-PCL (PEG Mw = 5000, PCL Mw = 5240) were obtained from Xi'an Ruixi Biological Technology Co., Ltd. Disodium telluride and di(1-hydroxylundecyl) ditelluride were prepared according to the previous reports .…”

Ocular delivery of proteins and peptides: Challenges and novel formulation approaches

“…Patients with neovascular glaucoma and iris rubeosis have also responded well to the intracameral bevacizumab therapy and did not show any morphological changes of corneal endothelial cells [119–121]. Intracameral injection of bevacizumab loaded polymeric delivery systems may sustain drug release into the anterior segment [122]. However, repeated injections to maintain therapeutic concentrations over prolonged time period and sparse degradation of polymers may obstruct the aqueous flow, thereby elevating intraocular pressure and risk of ocular infections.…”

Nanotechnology Applications for Glaucoma

“…Recently, an injectable thermosensitive hydrogel was generated for the administration of bevacizumab after filtration glaucoma surgery. 25 The rabbit models injected with polyethyleneglycol-poly-ε-caprolactone-polyethyleneglycol (PECE) hydrogel showed no sign of infection, ocular opacity, or any other ocular disease during follow-up. Drug-loaded hydrogels gradually degraded for a 3-week period and yielded lower postoperative IOPs compared with preoperative values.…”