Effects of bifidobacterial cytoplasm peptide and protein fractions on mouse lymphocyte proliferation and cytokine production

Amrouche, Tahar; Boutin, Yvan; Fliss, Ismaı̈l

doi:10.1080/09540100600565895

Cited by 8 publications

(7 citation statements)

References 45 publications

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“…Amrouche et al (2006a) showed that the SI of lymphocyte induced by cytoplasmic content and cell wall of Bifidobacterium lactis Bb12 at the concentrations of 200 lg protein ml -1 were significantly lower (P \ 0.05) than those at the concentrations of 20 and 40 lg protein ml -1 . Ghadimi et al (2008) thought that the optimum immunomodulating effect by live bacteria and DNA of several probiotic bacteria at bacteria/ PBMCs ratio of 10:1 and 75 lg DNA ml -1 was due to the average number of pathogen-associated molecular patterns receptors, such as Toll like receptors (TLRs) and nucleotide binding oligomerization domain (NODs) on the PBMCs.…”

Section: Pbmcs Proliferation Induced By Lactobacilli and Their Cell Cmentioning

confidence: 91%

“…The cell wall of the lactobacilli were prepared according to the method described by Heumann et al (1994) and Amrouche et al (2006a). Briefly, after 18 h incubation the bacterial cells from 50 ml of lactobacilli cultures were harvested by centrifugation (5,000g, 10 min, 4°C) and washed twice with PBS (pH 7.2).…”

Section: Preparation Of Cell Wall Of Bacterial Strainsmentioning

confidence: 99%

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In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk

Tuo

Zhang

Han

et al. 2010

World J Microbiol Biotechnol

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The objective of this study was to evaluate the in vitro immunomodulating capacity of Lactobacillus coryniformis subsp torquens T3L (L. coryniformis T3L) isolated from traditional fermented yak's milk in Tibet, China, and Lactobacillus paracasei supsp. paracasei M5L (L. paracasei M5L)isolated from kumiss in Sinkiang, China was used as control. The effects of live bacteria, cell wall and genomic DNA of the two Lactobacillus strains on human peripheral blood mononuclear cells (PBMCs) proliferation, production of interleukin-12 (IL-12 p70), interferon gamma (IFN-c) and tumor necrosis factor alpha (TNF-a), and natural killer (NK) cell activity were assessed. The live bacteria, cell wall and genomic DNA of the two lactobacilli exerted proliferative effects on PBMCs. Live bacteria at 1 9 10 6 c.f.u. ml -1 , cell wall at 20 lg protein ml -1 and DNA at 50 lg DNA ml -1 of the strainS induced the secretion of IL-12 (p70), IFN-c and TNF-a by PBMCs. NK cell activities increased after cultivation of PBMCs with live bacteria, cell wall and DNA of the strains. Overall, these results demonstrate that the live bacteria, cell wall and genomic DNA of the two Lactobacillus strains exhibit immunomodulating activity.

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Section: Pbmcs Proliferation Induced By Lactobacilli and Their Cell Cmentioning

confidence: 91%

Section: Preparation Of Cell Wall Of Bacterial Strainsmentioning

confidence: 99%

In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk

Tuo

Zhang

Han

et al. 2010

World J Microbiol Biotechnol

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“…The fraction exhibiting the highest proliferative activity from gel filtration chromatography was further purified according to the previous methods (Amrouche, Boutin, & Fliss, 2006;Cheng, Wang, Hsu, & Hwang, 2015). This fraction was purified using RP-HPLC on a SunFire TM C18 column (5 µm, 4.6 mm × 250 mm, Waters, USA).…”

Section: Rp-hplc Purificationmentioning

confidence: 99%

Purification and identification immunomodulatory peptide from rice protein hydrolysates

Mao

Huang

et al. 2019

Food and Agricultural Immunology

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2019) Purification and identification immunomodulatory peptide from rice protein hydrolysates, Food and Agricultural Immunology, 30:1, 150-162, ABSTRACTTo prepare immunomodulation peptides from rice protein hydrolysates (RPHs), trypsin was employed for enzymatic hydrolysis, and the immunomodulating activities of RPHs were studied using mouse peritoneal macrophage proliferation assay. The peptide fractions with the highest activity were further purified using macroporous adsorption resin, strong cation-exchange chromatography, gel filtration chromatography and reversed-phase high-performance liquid chromatography. The peptide sequence was identified as Tyr-Gly-Ile-Tyr-Pro-Arg (YGIYPR) by highperformance liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometer. Evaluating its immunomodulatory activity showed that YGIYPR enhanced the proliferation of macrophage RAW 264.7 cells in the range of 12.5-100 μg/mL, and even a minimum dose achieved good proliferation. The results demonstrated that the RPHs prepared by trypsin could serve as a source of immunomodulating peptides and the immunomodulatory peptide YGIYPR is a potential natural ingredient for manufacture of functional foods or health care products.

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“…Вивчення впливу різних фракцій цитоплазматичних пептидів та білків B. lactis виявило стимулювальний вплив похідних на про-ліферативну активність спленоцитів у разі застосування Alamar Blue ® як маркера клітинної проліферації (Amrouche et al, 2006). Вираженість стимулювального впливу залежала від фракціїi та мала концентраційнозалежний характер.…”

Section: обговоренняunclassified

“…Порівнюючи отримані у цій статті дані та результати досліджень впливу дериватів лакто-та біфідобактерій на метаболічну активність спленоцитів миші із застосуванням Alamar Blue ® -тесту інших авторів (Amrouche et al, 2006;Bauer et al, 2010;Kang et al, 2016), ми дійшли висновку: ефект похідних пробіотичних бактерій залежить від штаму, умов культивування пробіотика, способу отримання та біохімічного складу кінцевого продукту. Різноспрямований характер впливу дериватумісних продуктів на спленоцити у нашому (пригнічення) та вищезазначених дослідженнях (стимуляція) можна пояснити, спираючись на фундаментальну біомедичну парадигму гормезису, згідно з якою малі дози стимулюють, а великіінгібують біологічні показники (Shafran et al, 2010).…”

Section: обговоренняunclassified

Cellular metabolic activity as a marker of cytotoxicity and immunotropicity of probiotics’ derivatives

Knysh

Isayenko

Falko

et al. 2018

Regul. Mech. Biosyst.

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Structural components of cells and metabolites of probiotics with biologically active potential, along with the study of effectiveness, require a series of tests to ensure their safety. The study aims to test the cytotoxicity and potential of structural and metabolic derivatives of Bifidobacterium bifidum and Lactobacillus reuteri to affect the immunocompetent cells using in vitro tests that characterize the metabolic activity of test-cells. Structural components of probiotic bacteria were obtained by the physical method of disintegration – cyclic freezing-thawing. Metabolic derivatives were obtained by cultivation of producers – bifidobacteria and lactobacilli in their own disintegrates. Cultures of mouse embryonic fibroblasts and splenocytes were used as the test cells. MTT and Alamar Blue® were used as redox indicators. According to the MTT test, filtrates that contain structural and metabolic derivates at a concentration of 5% and 10% in the incubation medium did not cause significant changes in the metabolic activity of the embryonic mouse fibroblasts. An increase of up to 20% of content in the incubation medium of filtrates of lactobacilli disintegrates led to a reduction of metabolic activity of test cells by 52.7 ± 6.2%, of filtrates of bifidobacteria disintegrates – by 26.5 ± 6.5%, of filtrates of lactobacterium culture – by 15.7 ± 6.9%, of filtrates of bifidobacterium cultures - by 40.4 ± 6.8%. According to the Alamar Blue® test, filtrates that contained only structural derivatives of lactobacilli and bifidobacteria at concentrations of 5% and 10%, as well as filtrates that contained a complex of structural and metabolic derivatives at a concentration of 5%, did not cause significant changes in the reducing ability of mouse splenocytes. At concentrations of 10%, filtrate containing a complex of structural and metabolic derivatives of lactobacilli, caused the inhibition of metabolic activity of splenocytes by 14.6 ± 3.5%, and bifidobacteria – by 10.0 ± 2.8%. With the contents of the incubation medium at 20% concentration, the filtrate of the disintegrates of lactobacilli decreased the metabolic activity of splenocytes by 12.2 ± 3.0%, and the filtrate of lactobacillus cultures that were grown on their own disintegrates – by 43.2 ± 3.3%. Increasing the content of the disintegrate filtrate and the bifidobacteria culture that were grown on their own disintegrates in the culture medium by up to 20% led to a decrease the metabolic activity of splenocytes by 38.0 ± 2.0%. Thus, the research has shown: the orientation of changes in cellular metabolism under the influence of the studied biologically active derivatives is similar in all model systems, and their intensity depends on the type of test cells, regenerative substrates and concentration of the agent of influence in model systems. The obtained results stimulate further exploration of the immunotropicity of the investigated derivatives of probiotic bacteria and can be used for development of new immunobiological preparations.

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Effects of bifidobacterial cytoplasm peptide and protein fractions on mouse lymphocyte proliferation and cytokine production

Cited by 8 publications

References 45 publications

In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk

In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk

Purification and identification immunomodulatory peptide from rice protein hydrolysates

Cellular metabolic activity as a marker of cytotoxicity and immunotropicity of probiotics’ derivatives

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