Effects of bile acids and lectins on immunoglobulin production in rat mesenteric lymph node lymphocytes

Abstract

We investigated the effect of bile acids either alone or in combination with lectins on immunoglobulin (Ig) production in vitro of rat mesenteric lymph node (MLN) lymphocytes to examine their immunoregulatory activities. Among free bile acids examined, chenodeoxycholic acid stimulated IgE production by MLN lymphocytes and inhibited IgA production at the concentration of 0.3 mM, whereas cholic and deoxycholic acids exerted the comparable effect at 3 mM. Among conjugated bile acids, deoxycholic acid derivatives stimulated IgE production more strongly than cholic acid derivatives. On the other hand, free and conjugated bile acids did not affect IgG production. The IgE production by MLN lymphocytes was stimulated by concanavalin A and inhibited by pokeweed mitogen, and the effect of phytohemmagglutinin and lipopolysaccharide was marginal. These lectins did not affect IgA and IgG production by the lymphocytes. In the presence of lectins, free bile acids affected IgE production at 0.03 mM. These results suggest the possibility that bile acid is a stimulant for food allergy.

Effect of Tea Extracts and Phenolic Components on Immunoglobulin Production by Mesenteric Lymph Node Lymphocytes of Sprague-Dawley Rats.

“…Cells and cell culture MLN Iymphocytes were prepared from 9-to lO-week-old male Sprague-Dawley rats and cultured as previously described (Lim et al, 1994b). In short, MLN was excised from the rats under light diethyl ether anesthesia, and lymphocytes were isolated using LympholyteRat (Cedarlane, Hornby, Canada).…”

Effect of Dietary Level of Pectin on Immunoglobulin and Cytokine Production by Mesenteric Lymph Node Lymphocytes and Interleukin-2 Receptor in Rats.

“…In addition to ConA, several T-cell mitogens such as anti-CD3 antibodies and phytohemagglutinin have been used in immunologic studies. Among them, ConA has been reported to enhance IgE production of MLN lymphocytes in culture (Lim et al, 1994) and to induce the production of cytokines which enhance IgE production (Konno et al, 1993). These results suggest the dietary pectin regulates Ig production of rat lymphocytes through the regulation of cytokine, as well as ConA.…”

“…After 2 weeks of feeding rats were killed under diethyl ether anesthesia. MLN was excised and the lymphocytes were harvested (Lim et al, 1994).This experiment was carried out under the control of the guidelines for Animal Experiments in the Faculty of Agriculture and the Graduate Course of Kyushu University, and the Law (No. 105) and Notification (No.…”

“…6) of the Government in Japan. Lymphocytes (2¥10 6 cells/ml) were cultured in the RPMI 1640 medium supplemented with 10% FBS (Integren Co., NY) for 72 h at 37˚C and the immunoglobulin content of the culture supernatant was measured by ELISA as described previously (Lim et al, 1994). MLN lymphocytes were also cultured in the same medium in the presence or absence of Con A (25 g/ml), and the contents of IFN-␥ and TNF-␣ were measured by ELISA.…”

“…After 2 weeks of feeding rats were killed under diethyl ether anesthesia. MLN was excised and the lymphocytes were harvested (Lim et al, 1994).…”