Effects of bile salts and aliphatic ionic surfactants on human lymphocyte proliferation

Podevin, Philippe; Correia, L.; Montet, Jean-Claude; Conti, Filoména; Chéreau, Christiane; Calmus, Yvon; Poupon, Raoul

doi:10.1046/j.1365-2362.2001.00778.x

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…We found a significant negative correlation between the number of circulating DN T‐cells and the serum level of total bile acids in PBC patients ( r = −0.480, P < 0.001, Figure A). It has been reported that several serum bile acid levels were elevated in PBC, such as glycochenodeoxycholic acid, glycocholic acid and LCA, and bile salts can inhibit the cytokine production and proliferation of lymphocytes . The rank order for bile acid hydrophobicity is LCA > deoxycholic acid > chenodeoxycholic acid > UDCA .…”

Section: Resultsmentioning

confidence: 99%

“…It has been reported that several serum bile acid levels were elevated in PBC, such as glycochenodeoxycholic acid, glycocholic acid and LCA, 25 and bile salts can inhibit the cytokine production and proliferation of lymphocytes. 26,27 The rank order for bile acid hydrophobicity is LCA > deoxycholic acid > chenodeoxycholic acid > UDCA. 28 We therefore co-cultured PBMC with LCA, sodium glycochenodeoxycholate, sodium glycocholate hydrate and Figure 6D).…”

Section: Lca Downregulated Perforin Expression In Dn T-cells and Inmentioning

confidence: 99%

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Alteration of liver‐infiltrated and peripheral blood double‐negative T‐cells in primary biliary cholangitis

Zhang

et al. 2019

Liver International

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Background & Aims Double‐negative (DN) T‐cell is a unique regulatory T‐cell, which is essential for maintaining immune system homoeostasis. However, the role of DN T‐cells in the pathogenesis of primary biliary cholangitis (PBC) is still unknown. Methods We investigated the number and function of DN T‐cells in peripheral blood and liver biopsy specimens of PBC patients. Results The number and frequency of DN T‐cells significantly decreased in peripheral blood and liver tissue of PBC patients. Furthermore, the frequency of DN T‐cells in PBC was negatively correlated with disease severity and positively correlated with ursodeoxycholic acid response. In vitro assays showed that perforin expression and the suppressive capability of DN T‐cells on the proliferation of CD4+ and CD8+ T‐cells were impaired in PBC. Finally, lithocholic acid, the most hydrophobic acid, could downregulate the proliferation and perforin expression of DN T‐cells. Conclusions Decreased quantity and function of DN T‐cells in PBC may result in the loss of immune regulations on effector CD4+ and cytotoxic CD8+ T‐cells, and thereby may break the immune tolerance and promote the pathogenesis of PBC.

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Section: Resultsmentioning

confidence: 99%

Section: Lca Downregulated Perforin Expression In Dn T-cells and Inmentioning

confidence: 99%

Alteration of liver‐infiltrated and peripheral blood double‐negative T‐cells in primary biliary cholangitis

Zhang

et al. 2019

Liver International

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“…Similar phenomena have been described with other micelle forming compounds. Podevin et al [33] described the effect of bile salts on lymphocyte proliferation by claiming that the biological effect was partly due to the modification of the structure of the cell membrane lipid bilayer by the surface activity and hydrophobicity of bile salts. Similarly, Sudo et al [34] described that succinolyl trehalose lipid-3 (STL-3) inhibited the growth of Human HL-60 cells at or near the CMC (4 μM).…”

Section: Discussionmentioning

confidence: 99%

Association (micellization) and partitioning of aglycon triterpenoids

Rafat

Fong

Goldsipe

et al. 2008

Journal of Colloid and Interface Science

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“…Various hydrophobic compounds such as cholesterol, bile salts, and ionic surfactants have been previously shown to bind to and to change the composition of cellular membranes, causing changes in binding kinetics and related cellular consequences. 12,13 Compounds acting through further nonspecific modes might also be identified.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the InteraX™ System Technology in a High-Throughput Screening Environment

Büttner

Kumpf²,

Menzel³

et al. 2005

SLAS Discovery

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The authors have developed a cell-based high-throughput screening (HTS)-compatible assay to measure EGFR dimerization using the InteraX enzyme complementation technology of Applied Biosystems. The cells contain 2 chimeric proteins with complementing deletion mutants of the beta galactosidase enzyme, each fused to the extracellular and transmembrane part of EGFR. On binding of EGF, EGF receptor dimerizes and an active beta galactosidase is built. The authors used this homogeneous 384-well assay to screen about 20,000 diverse compounds. From 2 independent primary screen runs 239 hits were identified. For run 1, a mean S/B ratio of 4.26 and a mean Z' factor of 0.74 were obtained, for run 2 a mean S/B ratio of 3.88 and a mean Z' factor of 0.71 were obtained. After hit confirmation, repeated 4 times, 112 hits remained with a confirmation rate of 48.9%. Thirty of the 112 could be identified as cytotoxic. Fifty-one of the remaining 82 compounds could be shown to be inhibitors of the beta galactosidase enzyme itself. In summary, 31 compounds remained as potential EGFR dimerization or EGF stimulation inhibitors. The authors conclude that the InteraX system technology is HTS capable and can detect small molecule inhibitors capable of inhibiting protein-protein interactions.

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Effects of bile salts and aliphatic ionic surfactants on human lymphocyte proliferation

Abstract: Amphiphilic negatively charged molecules inhibit T-cell proliferation to an extent that is dependent upon their hydrophobicity. These results may be explained, at least in part, by a modification in the cell membrane lipid bilayer structure.

Cited by 7 publications

References 23 publications

Alteration of liver‐infiltrated and peripheral blood double‐negative T‐cells in primary biliary cholangitis

Alteration of liver‐infiltrated and peripheral blood double‐negative T‐cells in primary biliary cholangitis

Association (micellization) and partitioning of aglycon triterpenoids

Evaluation of the InteraX™ System Technology in a High-Throughput Screening Environment

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