Effects of bioactive‐rich extracts of pomegranate, persimmon, nettle, dill, kale and<i>Sideritis</i>and isolated bioactives on arachidonic acid induced markers of platelet activation and aggregation

Konić-Ristić, Aleksandra; Srdiċ-Rajiċ, Tatjana; Kardum, Nevena; Aleksic-Velickovic, Vesna; Kroon, Paul A.; Hollands, Wendy J; Needs, Paul W.; Boyko, N.; Hayran, Osman; Jorjadze, M.; Glibetić, Maria

doi:10.1002/jsfa.6328

Cited by 19 publications

(13 citation statements)

References 43 publications

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“…This data also supports a role for active biopeptides in modulating in vivo platelet function. The ability to inhibit platelet function has previously been confirmed ex vivo in the presence of other vegetable bioactives [27]. Nonetheless, it is unclear why this occurs or what role this response might play in pathological conditions, other than in healthy individuals with normal-high non-treated hypertension.…”

Section: Discussionmentioning

confidence: 95%

The Effect of Regular Intake of Dry-Cured Ham Rich in Bioactive Peptides on Inflammation, Platelet and Monocyte Activation Markers in Humans

et al. 2017

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Background and aims: Dietary studies have shown that active biopeptides provide protective health benefits, although the mediating pathways are somewhat uncertain. To throw light on this situation, we studied the effects of consuming Spanish dry-cured ham on platelet function, monocyte activation markers and the inflammatory status of healthy humans with pre-hypertension. Methods: Thirty-eight healthy volunteers with systolic blood pressure of >125 mmHg were enrolled in a two-arm crossover randomized controlled trial. Participants received 80 g/day dry-cured pork ham of >11 months proteolysis or 100 g/day cooked ham (control product) for 4 weeks followed by a 2-week washout before “crossing over” to the other treatment for 4 more weeks. Soluble markers and cytokines were analyzed by ELISA. Platelet function was assessed by measuring P-selectin expression and PAC-1 binding after ADP (adenosine diphosphate) stimulation using whole blood flow cytometry. Monocyte markers of the pathological status (adhesion, inflammatory and scavenging receptors) were also measured by flow cytometry in the three monocyte subsets after the interventional period. Results: The mean differences between dry-cured ham and cooked ham followed by a time period adjustment for plasmatic P-selectin and interleukin 6 proteins slightly failed (p = 0.062 and p = 0.049, respectively), notably increased for MCP-1 levels (p = 0.023) while VCAM-1 was not affected. Platelet function also decreased after ADP stimulation. The expression of adhesion and scavenging markers (ICAM1R, CXCR4 and TLR4) in the three subsets of monocytes was significantly higher (all p < 0.05). Conclusions: The regular consumption of biopeptides contained in the dry-cured ham but absent in cooked ham impaired platelet and monocyte activation and the levels of plasmatic P-selectin, MCP-1 and interleukin 6 in healthy subjects. This study strongly suggests the existence of a mechanism that links dietary biopeptides and beneficial health effects.

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Section: Discussionmentioning

confidence: 95%

The Effect of Regular Intake of Dry-Cured Ham Rich in Bioactive Peptides on Inflammation, Platelet and Monocyte Activation Markers in Humans

et al. 2017

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“…The reduction in platelet P-selectin expression, following QGPJ supplementation, signifies the ability of QGPJ to reduce platelet degranulation and inhibit platelet α-granule release consequently assisting in reducing thrombotic risk. Similarly, in subjects with metabolic syndrome, polyphenol-rich extracts of kale and pomegranate have reduced P-selectin and GPIIb-IIIa expression in vitro (Konic-Ristic et al, 2013). QGPJ or PJ did not alter PAC-1 expression and consequently had no effect on the initial phase of activation involving the GPIIb-IIIa receptor.…”

Section: Discussionmentioning

confidence: 98%

Consumption of anthocyanin-rich Queen Garnet plum juice reduces platelet activation related thrombogenesis in healthy volunteers

Santhakumar

Kundur

Fanning³

et al. 2015

Journal of Functional Foods

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Available online A B S T R A C TThe anti-thrombotic properties of an anthocyanin-rich Queen Garnet plum juice (QGPJ) and anthocyanin-free prune juice (PJ) were studied in this randomised, double-blind, crossover trial. Twenty-one healthy subjects (M = 10, F = 11) consumed QGPJ, PJ or placebo, 200 mL/ day for 28-days followed by a 2-week wash-out period. Only QGPJ supplementation inhibited platelet aggregation induced by ADP (<5%, P = 0.02), collagen (<2.7%, P < 0.001) and arachidonic acid (<4%, P < 0.001); reduced platelet activation-dependent surface-marker P-selectin expression of activated de-granulated platelets (<17.2%, P = 0.04); prolonged activatedpartial thromboplastin clotting time (>2.1 s, P = 0.03); reduced plasma-fibrinogen (<7.5%, P = 0.02) and malondialdehyde levels, a plasma biomarker of oxidative stress (P = 0.016). PJ supplementation increased plasma hippuric acid content (P = 0.018). QGPJ or PJ supplementation did not affect blood cell counts, lipid profile, or inflammation markers. Our findings suggest that QGPJ but not PJ has the potential to significantly attenuate thrombosis by reducing platelet activation/hyper-coagulability and oxidative stress.

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“…Thus, GPVI is involved in the protective effects observed with SFN treatment. It is interesting to note that SFN has already been previously shown to significantly inhibit platelet-neutrophil aggregation (along with P-selectin and GPIIb-IIIa expression) in subjects with metabolic syndrome versus healthy controls (Konić-Ristić et al, 2013). With respect to our study, whilst the effects of SFN on the interactions between leukocytes and platelets are not the main aim of our study, we found that SFN reduces expression of GPIIbIIIa and Pselectin on platelets which may reduce platelet-leukocyte interactions through P-selectin inhibition by increasing levels of cAMP followed by inhibiting intracellular signals (such as the PI3K/Akt and PLCγ2-PKC-p47 cascades) and ultimately inhibiting platelet activation.…”

Section: Figurementioning

confidence: 98%

The isothiocyanate sulforaphane modulates platelet function and protects against cerebral thrombotic dysfunction

Gillespie

Holloway

Becker

et al. 2018

British J Pharmacology

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Effects of bioactive‐rich extracts of pomegranate, persimmon, nettle, dill, kale andSideritisand isolated bioactives on arachidonic acid induced markers of platelet activation and aggregation

Abstract: These data show that bioactive-rich extracts of kale and pomegranate that are consumed as traditional plant foods of Black Sea area countries were effective in modulating platelet function.

Cited by 19 publications

References 43 publications

The Effect of Regular Intake of Dry-Cured Ham Rich in Bioactive Peptides on Inflammation, Platelet and Monocyte Activation Markers in Humans

The Effect of Regular Intake of Dry-Cured Ham Rich in Bioactive Peptides on Inflammation, Platelet and Monocyte Activation Markers in Humans

Consumption of anthocyanin-rich Queen Garnet plum juice reduces platelet activation related thrombogenesis in healthy volunteers

The isothiocyanate sulforaphane modulates platelet function and protects against cerebral thrombotic dysfunction

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