Effects of biochar and organic substrates on biodegradation of polycyclic aromatic hydrocarbons and microbial community structure in PAHs-contaminated soils

Bao, Huanyu; Wang, Jinfeng; Zhang, He; Li, Jiao; Li, Hui; Wu, Fuyong

doi:10.1016/j.jhazmat.2019.121595

Cited by 135 publications

(39 citation statements)

References 75 publications

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“…This chemical is the monomer of an important plastic, namely polyvinylchloride (PVC). Dyadobacter and Ohtaekwangia, which both increased significantly in the plastisphere of PLA in Villum soil, were previously identified as potential biodegraders of xenobiotic pollutants (Willumsen et al, 2005;Tejeda-Agredano et al, 2013;Bao et al, 2020).…”

Section: Bacterial Key Taxa Associated With Plasticsmentioning

confidence: 98%

The “Plastisphere” of Biodegradable Plastics Is Characterized by Specific Microbial Taxa of Alpine and Arctic Soils

Rüthi

Bölsterli

Pardi-Comensoli

et al. 2020

Front. Environ. Sci.

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Plastic pollution poses a threat to terrestrial ecosystems, even impacting soils from remote alpine and arctic areas. Biodegradable plastics are a promising solution to prevent long-term accumulation of plastic litter. However, little is known about the decomposition of biodegradable plastics in soils from alpine and polar ecosystems or the microorganisms involved in the process. Plastics in aquatic environments have previously been shown to form a microbial community on the surface of the plastic distinct from that in the surrounding water, constituting the so-called "plastisphere." Comparable studies in terrestrial environments are scarce. Here, we aimed to characterize the plastisphere microbiome of three types of plastics differing in their biodegradability in soil using DNA metabarcoding. Polylactic acid (PLA), polybutylene adipate terephthalate (PBAT), and polyethylene (PE) were buried in two different soils, from the Swiss Alps and from Northern Greenland, at 15 • C for 8 weeks. While physico-chemical characteristics of the polymers only showed minor (PLA, PBAT) or no (PE) changes after incubation, a considerably lower α-diversity was observed on the plastic surfaces and prominent shifts occurred in the bacterial and fungal community structures between the plastisphere and the adjacent bulk soil not affected by the plastic. Effects on the plastisphere microbiome increased with greater biodegradability of the plastics, from PE to PLA. Copiotrophic taxa within the phyla Proteobacteria and Actinobacteria benefitted the most from plastic input. Especially taxa with a known potential to degrade xenobiotics, including Burkholderiales, Caulobacterales, Pseudomonas, Rhodococcus, and Streptomyces, thrived in the plastisphere of the Alpine and Arctic soils. In addition, Saccharimonadales (superphylum Patescibacteria) was identified as a key taxon associated with PLA. The association of Saccharibacteria with plastic has not been reported before, and pursuing this finding further may shed light on the lifestyle of this obscure candidate phylum. Plastic addition affected fungal taxa to a lesser extent since only few fungal genera such as Phlebia and Alternaria

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Section: Bacterial Key Taxa Associated With Plasticsmentioning

confidence: 98%

The “Plastisphere” of Biodegradable Plastics Is Characterized by Specific Microbial Taxa of Alpine and Arctic Soils

Rüthi

Bölsterli

Pardi-Comensoli

et al. 2020

Front. Environ. Sci.

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“…So far, different strategies have been approached for enhancing biodegradation and bioremediation (Raman and Chandra, 2009;Pimviriyakul et al, 2020). By promoting the growth of soil microbial degraders, soil amendments are being used in order to accelerate the removal rate of pollutants (Bao et al, 2020). However, the selection of the amendments is mostly based on trial and error.…”

Section: Introductionmentioning

confidence: 99%

Strategies for Enhancing in vitro Degradation of Linuron by Variovorax sp. Strain SRS 16 Under the Guidance of Metabolic Modeling

Dhakar

Zarecki

Bommel

et al. 2021

Front. Bioeng. Biotechnol.

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Phenyl urea herbicides are being extensively used for weed control in both agricultural and non-agricultural applications. Linuron is one of the key herbicides in this family and is in wide use. Like other phenyl urea herbicides, it is known to have toxic effects as a result of its persistence in the environment. The natural removal of linuron from the environment is mainly carried through microbial biodegradation. Some microorganisms have been reported to mineralize linuron completely and utilize it as a carbon and nitrogen source. Variovorax sp. strain SRS 16 is one of the known efficient degraders with a recently sequenced genome. The genomic data provide an opportunity to use a genome-scale model for improving biodegradation. The aim of our study is the construction of a genome-scale metabolic model following automatic and manual protocols and its application for improving its metabolic potential through iterative simulations. Applying flux balance analysis (FBA), growth and degradation performances of SRS 16 in different media considering the influence of selected supplements (potential carbon and nitrogen sources) were simulated. Outcomes are predictions for the suitable media modification, allowing faster degradation of linuron by SRS 16. Seven metabolites were selected for in vitro validation of the predictions through laboratory experiments confirming the degradation-promoting effect of specific amino acids (glutamine and asparagine) on linuron degradation and SRS 16 growth. Overall, simulations are shown to be efficient in predicting the degradation potential of SRS 16 in the presence of specific supplements. The generated information contributes to the understanding of the biochemistry of linuron degradation and can be further utilized for the development of new cleanup solutions without any genetic manipulation.

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“…Corn straw and soil A PAHs-contaminated soil at a depth of 0-20 cm was obtained from the surrounding of a coal-power plant with 59 years history and the detailed description of location was reported in our previous study (Bao et al, 2020). Soil pH was determined with a pH meter (water: soil = 2.5:1, w/v, pH = 8.37).…”

Section: Methodsmentioning

confidence: 99%

“…5, the potential hosts of PAH-RHDα genes were Lysobacter, Rhizobium, Bacillus, Devosia, Ohtaekwangia, Ramlibacter, Massilia, Steroidobacter, Phenylobacterium and Microvirga. It is reported that Ohtaekwangia, Bacillus, Lysobacter and Rhizobium had the ability to degrade PAHs (Bao et al 2020). Devosia was abundant in crude oil and might play important roles in the degradation of asphaltene in soils (Song et al 2018).…”

Section: Changes In Bacterial Community Structuresmentioning

confidence: 99%

Enhanced rhizodegradation of polycyclic aromatic hydrocarbons in corn straw-amended soil related to changing of bacterial community and functional gene expression

Bao

Wang

Zhang

et al. 2021

Preprint

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Root exudates can stimulate microbial degradation in rhizosphere, but it remains unclear whether the rhizodegradation of polycyclic aromatic hydrocarbons (PAHs) occurs in corn straw-amended soil. Hence, in the present study, either citric acid, a common low molecular weight organic acid in the root exudates, or corn straw was added into aged PAHs-contaminated soil to investigate their effectiveness in the biodegradation of PAHs. The present study showed that either corn straw (Y) or combined application of corn straw and citric acid (YN100) significantly (P < 0.05) enhanced the degradation of total PAHs in soil after 28 days incubation, which increased by 8.43% and 18.62% compared with control (CK), respectively. High-throughput sequencing suggested that both Y and YN100 treatments led to a shift in bacterial community in soil and increased the abundance of PAHs degraders. Interestingly, the copies of PAHs ring-hydroxylating-dioxygenase (PAH-RHD) Gram-negative bacteria (GN) genes under YN100 treatment was significantly (P < 0.05) higher than those under Y treatment in the soil. Network analysis showed that the potential hosts of PAH-RHDα genes were Lysobacter, Rhizobium, Bacillus, Devosia, Ohtaekwangia, Ramlibacter, Massilia, Steroidobacter, Phenylobacterium and Microvirga. Bacillus, Lysobacter, Rhizobium and Ohtaekwangia and all ten genera obviously increased under Y and YN100 treatments. These results indicate that combined application of corn straw and citric acid increased the PAH-degrading bacteria and PAH-RHDα genes, thus improving the biodegradability of PAHs in the soil. As these results verified, a combined corn straw-rhizosphere approach should be a feasible remediation strategy for PAHs-contaminated soil.

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Effects of biochar and organic substrates on biodegradation of polycyclic aromatic hydrocarbons and microbial community structure in PAHs-contaminated soils

Cited by 135 publications

References 75 publications

The “Plastisphere” of Biodegradable Plastics Is Characterized by Specific Microbial Taxa of Alpine and Arctic Soils

The “Plastisphere” of Biodegradable Plastics Is Characterized by Specific Microbial Taxa of Alpine and Arctic Soils

Strategies for Enhancing in vitro Degradation of Linuron by Variovorax sp. Strain SRS 16 Under the Guidance of Metabolic Modeling

Enhanced rhizodegradation of polycyclic aromatic hydrocarbons in corn straw-amended soil related to changing of bacterial community and functional gene expression

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