Effects of biofertilizer and manure application on growth rate and chlorophyll content of spinach (<i>Amaranthus tricolor</i> L.) under salinity stress condition

Siswanti, Dwi Umi; Riesty, Okky Shavira

doi:10.1051/bioconf/20213305003

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…It includes a variety of physiological activities as cell division, elongation, differentiation, and photosynthesis [11]. Understanding the elements that affect plant development, such as environmental circumstances, genetic features, and management approaches, is critical for improving crop yields, forest productivity, and overall ecosystem health [12]. Yield, on the other hand, refers to the amount or quality of the finished product gathered from a crop, forest stand, or economic activity [13].…”

Section: Introductionmentioning

confidence: 99%

Impact of bio-fertilizers on growth, yield and quality of Spinach (Spinacia oleraceaL.) in Noida UP.

Adison,

Maisnam,

Devi

et al. 2024

BIO Web Conf.

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A field trial was undertaken at Amity University Noida’s experimental farm to investigate the effectiveness of microbial consortia on plant development and yield characteristics of spinach (Spinacia oleracea L.). Azotobacter, poultry manure, vermicompost, and FYM were used in various combinations throughout the treatments. The Desi (indigenous) variety was used in the field investigation. The use of biofertilizers is intended to increase crop yield and soil fertility while minimising environmental damage. The purpose of this study is to investigate the effects of various biofertilizers on the growth and yield of spinach (Spinacia oleracea L.) plants. These fertilisers include vermicompost, farmyard manure, Azotobacter, and poultry manure. The experiment was conducted out under closely monitored settings, with a randomised complete block design and three replications. Growth metrics were examined during the experiment to evaluate the vegetative growth of spinach plants, including plant height, leaf area, leaf length, and number of leaves per plant. The study’s findings demonstrated that the various treatments that were applied had a discernible impact on the growth, production, and economics of spinach. According to early data, the usage of biofertilizers considerably affected the growth and yield of spinach plants when compared to the control group. In comparison to the control, which had plant height (26.4 cm), leaves number (14), length of the leaves (15cm), leaves width (8cm), area of leaf per plant (120 cm2), and yield (4.5 Kg) the combined application of (Azotobacter + farmyard manure + poultry manure) demonstrated the highest performance on plant growth parameters such as height of the plants (35.4cm), leaves number per plant (16.7cm), leaves length (22cm), leaves width (12cm), and leaf area per plant (264 cm2). Furthermore, as seen by higher nutrient levels and organic matter content, the application of biofertilizers significantly improved soil fertility. This research present to the growing body of valuable information on sustainable farming practices by elucidating the efficacy of biofertilizers in enhancing the spinach growth and yield. The findings highlight how important it is to incorporate biofertilizers into agricultural practices to enhance soil health, reduce adverse environmental effects, and ensure the production of food sustainably.

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Section: Introductionmentioning

confidence: 99%

Impact of bio-fertilizers on growth, yield and quality of Spinach (Spinacia oleraceaL.) in Noida UP.

Adison,

Maisnam,

Devi

et al. 2024

BIO Web Conf.

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Relative Expression of a Salinity Stress‐Responsive Na⁺/H⁺ Exchanger (NHX) in Root and Leaf Tissues of the African Leafy Vegetable, Amaranthus dubius

Haripershad,

Nakhooda,

Shaik

2024

Food and Energy Security

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Amaranthus dubius, an African leafy vegetable (ALV), is an easy‐to‐grow, annual shrub and a highly nutritious food source, containing elevated levels of essential nutrients in the leaves. Many ALVs, including A. dubius, can tolerate salinity stress, enabling their cultivation on marginal land. However, the widespread propagation of A. dubius as a stable food source has thus far not been realised due partially to the high frequency at which hybridisation occurs, resulting in high genotypic and phenotypic variability. Therefore, to increase the agricultural output capacity of this species on salt‐affected marginal lands, it is important to screen, select and then clonally propagate the identified salinity‐tolerant genotypes to ensure true‐to‐type fidelity in the regenerated population. It is also important, thereafter, to elucidate their underlying gene expression of the stress response. The present study exposed 4‐week‐old A. dubius seedlings to 100, 200 and 400 mM NaCl to determine their degree of salt tolerance. Genotypes were then screened, selected and clonally propagated through cuttings, based on high growth rates and biomass, and salt tolerance. Generally, growth and physiological parameters decreased as substrate salinity increased. However, individual salt‐stressed genotypes demonstrated similar vigour to nonstressed plants and were able to maintain total protein and chlorophyll concentrations despite increasing salinity. The relative expression of an NHX1‐like transcript was quantified in 15 genotypes using degenerately primed real‐time qPCR. The relative expression of the putative NHX1 gene was 6.7 times greater in root tissues of seedlings treated with 400 mM NaCl (10.7 ± 1.8) compared to the roots of untreated seedlings (1.6 ± 1.3), and 2.8‐fold more than leaf tissues harvested from seedlings treated with 400 mM NaCl. Furthermore, the relative electrical conductivity (EC) of root tissues was 10 times greater than the EC of leaf tissues from the same 400 mM NaCl treatment. Numerous genotypes yielded similar chlorophyll content between 200 and 400 mM NaCl treatments, with genotypes salinity‐1 (S1) (3.5 ± 0.2 μg/cm2) and S34 (4.0 ± 0.4 μg/cm2) having the highest concentrations of chlorophyll in the 400 mM group, which was positively correlated with total protein content. Following micropropagation through direct organogenesis, selected clones maintained true‐to‐type traits such as similar chlorophyll, protein and NHX1‐like expression as their parent plants when exposed to 400 mM NaCl. This study revealed that some genotypes demonstrated salt stress tolerance capabilities rivalling established halophytes by regulating the constitutive or inducible expression of an NHX1‐like protein in roots and leaves. The correlation between protein content and NHX1‐like expression was nonlinear and nonproportional, demonstrating the complexity of this response and necessitating further exploration of specific protein families or functional groups conferring salinity tolerance in this species.

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Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment

Dimitrova,

Kaiyrbekov,

Balabanova

2024

EJSS

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Biofertilizers, consisting of carefully selected microorganisms across various species and genera, exhibit distinct features that enhance soil fertility and promote plant growth. Embracing the principles of eco-friendly agriculture, the use of biofertilizers emerges as a pivotal strategy for sustainable farming, contributing to environmental preservation and the overall health and biodiversity of the soil. In this study, a commercially available biofertilizer, containing a specialized strain of Priestia megatherium with nitrogen-fixing capabilities, was employed alongside chemical fertilizers at two different doses (30 and 40 mg per kg of soil). The primary objective was to evaluate the impact of biofertilizer on the metabolic activity and structure of microbial communities in a short-term experiment involving potted maize plants, utilizing the BIOLOG® EcoPlates technique. Parameters such as average well-color development (AWCD) and substrate utilization across six guilds (SAWCD) were assessed to gauge microbial metabolic activity. Additionally, functional indexes, including Shannon diversity, Shannon evenness, and Simpson diversity, were calculated as indicators of soil microbial community functionality. While statistically significant differences in AWCD among the studied variants were not observed, all estimated functional indexes consistently revealed heightened microbial diversity and evenness following the application of biofertilizer. This noteworthy finding, achieved within a relatively short period of plant cultivation, underscores the necessity for further research to explore the biofertilizer's enduring effects on soil communities, both in controlled laboratory environments and under real-world field conditions.

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Effects of biofertilizer and manure application on growth rate and chlorophyll content of spinach (Amaranthus tricolor L.) under salinity stress condition

Cited by 4 publications

References 6 publications

Impact of bio-fertilizers on growth, yield and quality of Spinach (Spinacia oleraceaL.) in Noida UP.

Impact of bio-fertilizers on growth, yield and quality of Spinach (Spinacia oleraceaL.) in Noida UP.

Relative Expression of a Salinity Stress‐Responsive Na⁺/H⁺ Exchanger (NHX) in Root and Leaf Tissues of the African Leafy Vegetable, Amaranthus dubius

Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment

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Effects of biofertilizer and manure application on growth rate and chlorophyll content of spinach (Amaranthus tricolor L.) under salinity stress condition

Cited by 4 publications

References 6 publications

Impact of bio-fertilizers on growth, yield and quality of Spinach (Spinacia oleraceaL.) in Noida UP.

Impact of bio-fertilizers on growth, yield and quality of Spinach (Spinacia oleraceaL.) in Noida UP.

Relative Expression of a Salinity Stress‐Responsive Na+/H+ Exchanger (NHX) in Root and Leaf Tissues of the African Leafy Vegetable, Amaranthus dubius

Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment

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Relative Expression of a Salinity Stress‐Responsive Na⁺/H⁺ Exchanger (NHX) in Root and Leaf Tissues of the African Leafy Vegetable, Amaranthus dubius