2022
DOI: 10.1038/s41467-022-33134-x
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Effects of biological sex mismatch on neural progenitor cell transplantation for spinal cord injury in mice

Abstract: Despite advancement of neural progenitor cell transplantation to spinal cord injury clinical trials, there remains a lack of understanding of how biological sex of transplanted cells influences outcomes after transplantation. To address this, we transplanted GFP-expressing sex-matched, sex-mismatched, or mixed donor cells into sites of spinal cord injury in adult male and female mice. Biological sex of the donor cells does not influence graft neuron density, glial differentiation, formation of the reactive gli… Show more

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Cited by 12 publications
(11 citation statements)
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“…We included dorsal and ventral E12.5 NPC groups because we and others have previously shown that transplantation of dorsoventrally restricted rat spinal cord NPCs results in significant differences in graft cellular composition and immunoreactivity for specific axon fiber subtypes 12 , 23 , 48 ; hence, this serves as a useful strategy for manipulating graft composition. At four weeks post-transplantation, all grafts exhibited strong GFP fluorescence and complete filling of the lesion site, with a typical degree of vascularization 49 (Fig. 2b ).…”
Section: Resultsmentioning
confidence: 95%
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“…We included dorsal and ventral E12.5 NPC groups because we and others have previously shown that transplantation of dorsoventrally restricted rat spinal cord NPCs results in significant differences in graft cellular composition and immunoreactivity for specific axon fiber subtypes 12 , 23 , 48 ; hence, this serves as a useful strategy for manipulating graft composition. At four weeks post-transplantation, all grafts exhibited strong GFP fluorescence and complete filling of the lesion site, with a typical degree of vascularization 49 (Fig. 2b ).…”
Section: Resultsmentioning
confidence: 95%
“…We utilized a cervical (C5) dorsal column lesion for graft phenotypic characterization (Figs. 2 – 5 , S 2 –S 4 ), because this SCI model enables greater ease of animal care and supports graft survival without a stabilizing matrix 12 , 49 . Mice were deeply anesthetized, and the dorsal skin between the shoulder blades was shaved and scrubbed with betadine and 70% ethanol.…”
Section: Methodsmentioning
confidence: 99%
“…Pitonak et al 7 begins to illuminate such sources of variability in cell grafting approaches by highlighting the importance of biological sex. In this study, we utilized a model of “mouse-to-mouse” grafting in which adult C57BL/6 mice received donor cells obtained from embryos of the same syngeneic strain.…”
mentioning
confidence: 99%
“…Through this work, we showed that female host animals that received male donor cells exhibited hypervascularization, increased perivascular cell density and heightened infiltration of T cells compared with other experimental groups, demonstrating a cellular inflammatory response in sex-mismatched female recipients, but not males. 7 …”
mentioning
confidence: 99%
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