Effects of biomaterial-induced inflammation on fibrosis and rejection

Jones, Kim S.

doi:10.1016/j.smim.2007.11.005

Cited by 133 publications

(107 citation statements)

References 54 publications

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“…The biocompatibility of the scaffolds after implantation depends on severity and type of immune reaction. Inflammatory responses occur in all surgical injuries and usually resolved up to couple of weeks [50][51][52]. In this study, the selected scaffold was implanted subcutaneously, removed and sectioned after prescribed time and stained with H&E (Fig.…”

Section: In Vivo Biocompatibility Assaymentioning

confidence: 99%

Optimization of nanofibrous silk fibroin scaffold as a delivery system for bone marrow adherent cells: in vitro and in vivo studies

Gholipourmalekabadi

Mozafari

Bandehpour

et al. 2015

Biotech and App Biochem

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Electrospun silk fibroin nanofibrous scaffolds (ESFNSs) were successfully prepared by electrospinning of various Bombyx mori silk fibroin concentrations (10, 12, and 14% in formic acid). After characterizing the purified silk fibroin, the morphology, porosity, fibers' diameter, and uniformity of the prepared scaffolds were examined in detail. In addition, biological responses such as effects on bone marrow cell viability, cytotoxicity, and cell adhesion were evaluated in vitro. Biocompatibility and bioactivity properties of the ESFNSs were evaluated in vitro and in vivo by cell culturing and subcutaneous implantation in rat models for 7 and 28 days, respectively. According to the obtained results, no beaded fibers were seen in any of the prepared scaffolds, whereas ESFNS-10% provided more uniformity and porosity with nanoscaled fibers (90 ± 0.021 nm). Furthermore, the scaffolds also showed good cell adhesion and spreading (68.7 ± 11.8 and 7.6 ± 3.3 total length and width, respectively) with no detectable effect on cell viability and cytotoxicity. The in vivo biocompatibility evaluation indicated that the scaffolds did not stimulate detectable cellular inflammatory response (lymphocytes) and increased the total cell number (cellularity) in the implantation area. Furthermore, the results suggest the potential use of the prepared ESFNS-10% bone marrow cell constructs in direct implantation for tissue engineering applications.

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Section: In Vivo Biocompatibility Assaymentioning

confidence: 99%

Optimization of nanofibrous silk fibroin scaffold as a delivery system for bone marrow adherent cells: in vitro and in vivo studies

Gholipourmalekabadi

Mozafari

Bandehpour

et al. 2015

Biotech and App Biochem

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“…Such response may hamper the therapeutic potential of the transplanted patch and can lead to graft rejection and adversely affect the health of the patient. 6,7 To address this, we have recently suggested that nonimmunogenic scaffolds can be fabricated from the patient's own biomaterials. We have shown that parts of the omentum can be easily, quickly, and safely harvested from the patient, and after efficient cell removal and quick processing, a porous 3D scaffold can be obtained.…”

mentioning

confidence: 99%

Gold Nanoparticle-Decellularized Matrix Hybrids for Cardiac Tissue Engineering

et al. 2014

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Decellularized matrices are valuable scaffolds for engineering functional cardiac patches for treating myocardial infarction. However, the lack of quick and efficient electrical coupling between adjacent cells may jeopardize the success of the treatment. To address this issue, we have deposited gold nanoparticles on fibrous decellularized omental matrices and investigated their morphology, conductivity, and degradation. We have shown that cardiac cells engineered within the hybrid scaffolds exhibited elongated and aligned morphology, massive striation, and organized connexin 43 electrical coupling proteins. Finally, we have shown that the hybrid patches demonstrated superior function as compared to pristine patches, including a stronger contraction force, lower excitation threshold, and faster calcium transients.

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“…It has been reported that LC is infiltrated into the implanted site and attached to the non-biocompatible materials. In a severe immune reaction, materials are attacked by LC that results in anaphylactic shock and implant rejection (Coleman et al, 1974;Jones, 2008;Krammer et al, 2007). Therefore, counting the number of such cells is an accurate indicator in investigation of the biocompatibility of materials (Anderson et al, 2008;Gholipourmalekabadi et al, 2014b).…”

Section: In Vivo Biocompatibilitymentioning

confidence: 99%