Effects of Biosilicate<sup>®</sup> Scaffolds and Low-Level Laser Therapy on the Process of Bone Healing

Pinto, Karina Nogueira Zambone; Tim, Carla Roberta; Crovace, Murilo C.; Matsumoto, Mariza Akemi; Parizotto, Nivaldo Antônio; Zanotto, Edgar Dutra; Peitl, Oscar; Rennó, Ana Cláudia Muniz

doi:10.1089/pho.2012.3435

Cited by 38 publications

(49 citation statements)

References 29 publications

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“…Conversely, Renno, McDonnell, Crovace, Zanotto, and Laakso (2010) used a 10 J/cm 2 dose to evaluate the proliferation of MC3T3‐E1 cells cultured on biosilicate and noted decreased cell proliferation when compared with the non‐irradiated group, possibly due to biomaterial interferences (light refraction or light absorption) in laser action. Similar results were observed in the study carried out by Pinto et al (2013), who also used a biosilicate scaffold and a 120 J/cm 2 dose for repairing bone defects in rats and did not identify an extra laser therapy effect on the regeneration process. Light refraction or absorption phenomena by the biomaterial used by the authors do not seem to be relevant when using PLA films, as the present study obtained a much higher cell proliferation in the irradiated group compared with the non‐irradiated group.…”

Section: Discussionsupporting

confidence: 88%

Laser therapy increases the proliferation of preosteoblastic MC3T3‐E1 cells cultured on poly(lactic acid) films

Sabino

Ginani

Silva

et al. 2020

J Tissue Eng Regen Med

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This study aimed to verify the efficacy of low‐level laser irradiation (LLLI) on the proliferation of MC3T3‐E1 preosteoblasts cultured on poly(lactic acid) (PLA) films. The produced films were characterized by contact angle tests, scanning electron microscopy (SEM), atomic force microscopy, differential scanning calorimetry, and X‐ray diffraction. The MC3T3‐E1 cells were cultured as three different groups: Control—cultured on polystyrene plastic surfaces; PLA—cultured on PLA films; and PLA + Laser—cultured on PLA films and submitted to laser irradiation (660 nm; 30 mW; 4 J/cm2). Cell proliferation was analyzed by Trypan blue and Alamar blue assays at 24, 48, and 72 h after irradiation. Cell viability was assessed by Live/Dead assay, apoptosis‐related events were evaluated by Annexin V/propidium iodide (PI) expression, and cell cycle events were analyzed by flow cytometry. Cell morphology on the surface of films was assessed by SEM. Cell counting and biochemical assay results indicate that the PLA + Laser group exhibited higher proliferation (p < 0.01) when compared with the Control and PLA groups. The Live/Dead and Annexin/PI assays indicate increased cell viability in the PLA + Laser group that also presented a higher percentage of cells in the proliferative cell cycle phases (S and G2/M). These findings were also confirmed by the higher cell density observed in the irradiated group through SEM images. The evidence from this study supports the idea that LLLI increases the proliferation of MC3T3‐E1 cells on PLA surfaces, suggesting that it can be potentially applied in bone tissue engineering.

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Section: Discussionsupporting

confidence: 88%

Laser therapy increases the proliferation of preosteoblastic MC3T3‐E1 cells cultured on poly(lactic acid) films

Sabino

Ginani

Silva

et al. 2020

J Tissue Eng Regen Med

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“…Four articles employed inorganic bovine bone graft to conduct their experiments [42][43][44][45] while one of the studies used lyophilized organic bovine bone [46]. Biosilicate was utilized in five articles [47,49,53,57,64]. In three studies, mineral tri-oxide aggregate (MTA) was used [55][56][57].…”

Section: Bone Substitutes Utilizedmentioning

confidence: 99%

Effects of photobiomodulation on bone defects grafted with bone substitutes: A systematic review of in vivo animal studies

Hanna

Dalvi

Amaroli

et al. 2020

Journal of Biophotonics

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A present, photobiomodulation therapy (PBMT) effectiveness in enhancing bone regeneration in bone defects grafted with or without biomaterials is unclear. This systematic review (PROSPERO, ref. CRD 42019148959) aimed to critically appraise animal in vivo published data and present the efficacy of PBMT and its potential synergistic effects on grafted bone defects. MEDLINE, CCCT, Scopus, Science Direct, Google Scholar, EMBASE, EBSCO were searched, utilizing the following keywords: bone repair; low-level laser therapy; LLLT; light emitting diode; LEDs; photobiomodulation therapy; in vivo animal studies, bone substitutes, to identify studies between 1994 and 2019. After applying the eligibility criteria, 38 papers included where the results reported according to "PRISMA." The results revealed insufficient and incomplete PBM parameters, however, the outcomes with or without biomaterials have positive effects on bone healing. In conclusion, in vivo animal studies with a standardized protocol to elucidate the effects of PBMT on biomaterials are required initially prior to clinical studies.

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“…Melatonin was injected intraperitoneally. After exposing the right proximal tibia of each animal, a standardized 6.0 mm diameter non-critical bone defect was created by using a motorized drill under irrigation with saline solution (Oliveira et al, 2010;Granito et al, 2011;Pinto et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

Effects of Melatonin on Tibia Bone Defects in Rats

et al. 2016

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KOPARAL, M.; IRTEGÜN, S.; ALAN, H.; DEVECI, E.; GÜLSÜN, B. & PEKTANÇ, G.Effects of melatonin on tibia bone defects in rats. Int. J. Morphol., 34(2):763-769, 2016. SUMMARY:The aim of this study was to evaluate the effects of melatonin healing in a tibial bone defect model in rats by means of histopathological and immunohistochemistry analysis. Twenty one male Wistar albino rats were used in this study. In each animal, bone defects (6 mm length ) were created in the tibias. The animals were divided into three groups. In group 1 control group (rats which tibial defects). Group 2 melatonin (10 mg/kg) + 14 days in the tibial defect group) was administered intraperitoneally to rats. Group 3 melatonin (10 mg/kg) + 28 days in the tibial defect group) was administered intraperitoneally to rats. Histopathological analysis of samples was performed to evaluate the process of osteoblastic activity, matrix formation, trabecular bone formation and myeloid tissue in bone defects. Immunohistochemical and immunoblot analysis demonstrated non-collagenous proteins (osteopontin and osteonectin) differences in tibial bone defects. The expression of osteopontin on tibia was increased by 14 days melatonin treatment. The expression of osteonectin on tibia was dramatically increased by 14 days melatonin treatment.

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Effects of Biosilicate^® Scaffolds and Low-Level Laser Therapy on the Process of Bone Healing

Abstract: Our findings suggest that Biosilicate presented osteogenic activity, accelerating bone repair. However, laser therapy was not able to enhance the bioactive properties of the Biosilicate.

Cited by 38 publications

References 29 publications

Laser therapy increases the proliferation of preosteoblastic MC3T3‐E1 cells cultured on poly(lactic acid) films

Laser therapy increases the proliferation of preosteoblastic MC3T3‐E1 cells cultured on poly(lactic acid) films

Effects of photobiomodulation on bone defects grafted with bone substitutes: A systematic review of in vivo animal studies

Effects of Melatonin on Tibia Bone Defects in Rats

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Effects of Biosilicate® Scaffolds and Low-Level Laser Therapy on the Process of Bone Healing

Abstract: Our findings suggest that Biosilicate presented osteogenic activity, accelerating bone repair. However, laser therapy was not able to enhance the bioactive properties of the Biosilicate.

Cited by 38 publications

References 29 publications

Laser therapy increases the proliferation of preosteoblastic MC3T3‐E1 cells cultured on poly(lactic acid) films

Laser therapy increases the proliferation of preosteoblastic MC3T3‐E1 cells cultured on poly(lactic acid) films

Effects of photobiomodulation on bone defects grafted with bone substitutes: A systematic review of in vivo animal studies

Effects of Melatonin on Tibia Bone Defects in Rats

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Product

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Effects of Biosilicate^® Scaffolds and Low-Level Laser Therapy on the Process of Bone Healing