Effects of Biotin Supplementation in the Diet on Adipose Tissue cGMP Concentrations, AMPK Activation, Lipolysis, and Serum-Free Fatty Acid Levels

Boone-Villa, Daniel; Aguilera‐Méndez, Asdrúbal; Miranda-CervantesAdriana,; Fernandez-MejiaCristina,

doi:10.1089/jmf.2014.0170

Cited by 19 publications

(34 citation statements)

References 45 publications

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“…This translates into a decrease in lipid synthesis rates and a relief malonyl CoA carnitine-palmitoyltransferase-1 inhibition, which leads to a higher rate of fatty acid oxidation. Consistent with the biotin-induced increase of active AMPK, we found that biotin supplementation increased the abundance of phosphorylated ACC-1 and -2 in the liver and adipose tissue [10,13]. Furthermore, we found that serum fatty acid concentrations decreased, but it did not affect lipolysis [10].…”

Section: Introductionsupporting

confidence: 84%

“…At pharmacological concentrations, biotin has other actions associated with changes in gene expression at the transcription, translation, and post-translation levels [1][2][3][4]. Several investigations have documented that pharmacological concentrations of biotin modify lipid homeostasis [5][6][7][8][9][10][11][12]. Studies on humans and rodents demonstrate that the administration of biotin at pharmacological doses decreases serum concentrations of triglycerides [7,12], cholesterol [5,6], and free fatty acid [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Effect of biotin supplementation on fatty acid metabolic pathways in 3T3‐L1 adipocytes

2018

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Several studies have shown that pharmacological concentrations of biotin decrease serum lipid concentrations and the expression of lipogenic genes. Previous studies on epididymal adipose tissue in mice revealed that 8 weeks of dietary biotin supplementation increased the protein abundance of the active form of AMPK and the inactive forms acetyl CoA carboxylase (ACC)‐1 and − 2, and decreased serum free fatty acid concentrations but did not affect lipolysis. These data suggest that pharmacological concentrations of the vitamin might affect fatty acid metabolism. In this work, we investigated the effects of pharmacological biotin concentrations on fatty acid synthesis, oxidation, and uptake in 3T3‐L1 adipocytes. Similar to observations in mice, biotin‐supplemented 3T3‐L1 adipose cells increased the protein abundance of active T172‐AMPK and inactive ACC‐1 and ‐2 forms. No changes were observed in the expression of the transcriptional factor PPARα and carnitine‐palmitoyltransferase‐1 (CPT‐1). Radiolabeled assays indicated a decrease in fatty acid synthesis; an increase in fatty acid oxidation and fatty acid incorporation rate into the lipid fraction between control cells and biotin‐supplemented cells. The data revealed an increase in the mRNA abundance of the fatty acid transport proteins Fatp1 and Acsl1 but not Cd36 or Fatp4 mRNA. Furthermore, the abundance of glycerol phosphate acyl transferase‐3 protein was increased. Triglyceride content was not affected. Lipid droplet numbers showed an increase and their areas were smaller in the biotin‐supplemented group. In conclusion, these data indicate that biotin supplementation causes a decrease in fatty acid synthesis and an increase in its oxidation and uptake. © 2018 BioFactors, 45(2):259–270, 2019

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Section: Introductionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Effect of biotin supplementation on fatty acid metabolic pathways in 3T3‐L1 adipocytes

2018

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“…At weaning three-week-old male Balb/cAnN mice were fed one of the following diets: biotin-control (TD-01362) or biotin-supplemented diet (TD-02458) containing 1.76 mg and 97.70 mg of free biotin/kg diet, respectively (Harlan Teklad, Madison WI, USA) as described previously [15–18, 26]. A third group of mice received the control diet but were treated with a single dose of carbon tetrachloride (CCl 4 , 2 ml/kg body weight) to induce liver damage.…”

Section: Methodsmentioning

confidence: 99%

“…Food consumption and body weight were measured through the experiment. After 8 weeks, the mice were deprived of food for 12 h and anesthetized as described in [15–18], and the blood and liver were extracted. The mice were killed via cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

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Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

Riverón-Negrete

Sicilia-Argumedo

Álvarez-Delgado

et al. 2016

BioMed Research International

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Pharmacological concentrations of biotin have pleiotropic effects. Several reports have documented that biotin supplementation decreases hyperglycemia. We have shown that a biotin-supplemented diet increased insulin secretion and the mRNA abundance of proteins regulating insulin transcription and secretion. We also found enlarged pancreatic islets and modified islet morphology. Other studies have shown that pharmacological concentrations of biotin modify tissue structure. Although biotin administration is considered safe, little attention has been given to its effect on tissue structure. In this study, we investigated the effect of biotin supplementation on hepatic morphology and liver toxicity markers. Male BALB/cAnN Hsd mice were fed a control or a biotin-supplemented diet for 8 weeks. Versus the control mice, biotin-supplemented mice had an altered portal triad with dilated sinusoids, increased vascularity, and bile conducts. Furthermore, we observed an increased proportion of nucleomegaly and binucleated hepatocytes. In spite of the liver morphological changes, no differences were observed in the serum liver damage indicators, oxidative stress markers, or antioxidant enzymes. Our data demonstrate for the first time that biotin supplementation affects liver morphology in normal mice, and that these modifications are not paralleled with damage markers.

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Elucidating the mechanisms underlying the anti‐hyperlipidemic effects of Laportea bulbifera using integrated serum metabolomics and network pharmacology

Shi

Lin

Huang

et al. 2023

Biomedical Chromatography

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Hyperlipidemia is a chronic metabolic disorder characterized by alterations in lipid metabolism as well as other pathways. Laportea bulbifera, an indigenous medicinal plant of Chinese herbal medicine, exhibits therapeutic effects on hyperlipidemia, but the mechanisms remain unclear. This study investigated the potential mechanisms underlying the anti‐hyperlipidemic effects of L. bulbifera using an integrated strategy based on metabolomics and network pharmacology methods that were established to investigate the potential mechanism of anti‐hyperlipidemia effect of L. bulbifera. First, the therapeutic effects of L. bulbifera on body weight reduction and biochemical indices were assessed. Next, 18 significant metabolites distinguishing the control and model groups were identified based on serum metabolomics and multivariate analyses. Then, a compound–target network was constructed by linking L. bulbifera and hyperlipidemia using network pharmacology. Three metabolic pathways involved in treating hyperlipidemia were identified. Finally, five crucial targets were selected by constructing a bionetwork starting from the compounds and ending in the metabolites. This study established an integrated strategy based on metabolomics coupled with network pharmacology and revealed the mechanism underlying the protective effects of L. bulbifera against hyperlipidemia for the first time.

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Effects of Biotin Supplementation in the Diet on Adipose Tissue cGMP Concentrations, AMPK Activation, Lipolysis, and Serum-Free Fatty Acid Levels

Cited by 19 publications

References 45 publications

Effect of biotin supplementation on fatty acid metabolic pathways in 3T3‐L1 adipocytes

Effect of biotin supplementation on fatty acid metabolic pathways in 3T3‐L1 adipocytes

Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

Elucidating the mechanisms underlying the anti‐hyperlipidemic effects of Laportea bulbifera using integrated serum metabolomics and network pharmacology

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