Effects of biotin supplementation in the diet on insulin secretion, islet gene expression, glucose homeostasis and beta-cell proportion

Lazo-de-la-Vega-Monroy, Maria-Luisa; Larrieta, Elena; German, Michael S.; Báez-Saldaña, Armida; Fernández-Mejía, Cristina

doi:10.1016/j.jnutbio.2012.03.020

Cited by 58 publications

(34 citation statements)

References 58 publications

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“…At weaning three-week-old male Balb/cAnN mice were fed one of the following diets: biotin-control (TD-01362) or biotin-supplemented diet (TD-02458) containing 1.76 mg and 97.70 mg of free biotin/kg diet, respectively (Harlan Teklad, Madison WI, USA) as described previously [15–18, 26]. A third group of mice received the control diet but were treated with a single dose of carbon tetrachloride (CCl 4 , 2 ml/kg body weight) to induce liver damage.…”

Section: Methodsmentioning

confidence: 99%

“…Food consumption and body weight were measured through the experiment. After 8 weeks, the mice were deprived of food for 12 h and anesthetized as described in [15–18], and the blood and liver were extracted. The mice were killed via cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

“…In previous studies in our laboratory, normal mice fed a biotin-supplemented diet for 8 weeks demonstrated increased insulin secretion and higher levels of mRNAs involved in the control of insulin transcription and secretion compared with mice fed a biotin-sufficient diet [15]. Interestingly, biotin supplementation induced an increase in islet size and modified its typical architecture of alpha-cells at the periphery and beta-cells at the core [15].…”

Section: Introductionmentioning

confidence: 98%

“…Interestingly, biotin supplementation induced an increase in islet size and modified its typical architecture of alpha-cells at the periphery and beta-cells at the core [15]. …”

Section: Introductionmentioning

confidence: 99%

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Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

Riverón-Negrete

Sicilia-Argumedo

Álvarez-Delgado

et al. 2016

BioMed Research International

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Pharmacological concentrations of biotin have pleiotropic effects. Several reports have documented that biotin supplementation decreases hyperglycemia. We have shown that a biotin-supplemented diet increased insulin secretion and the mRNA abundance of proteins regulating insulin transcription and secretion. We also found enlarged pancreatic islets and modified islet morphology. Other studies have shown that pharmacological concentrations of biotin modify tissue structure. Although biotin administration is considered safe, little attention has been given to its effect on tissue structure. In this study, we investigated the effect of biotin supplementation on hepatic morphology and liver toxicity markers. Male BALB/cAnN Hsd mice were fed a control or a biotin-supplemented diet for 8 weeks. Versus the control mice, biotin-supplemented mice had an altered portal triad with dilated sinusoids, increased vascularity, and bile conducts. Furthermore, we observed an increased proportion of nucleomegaly and binucleated hepatocytes. In spite of the liver morphological changes, no differences were observed in the serum liver damage indicators, oxidative stress markers, or antioxidant enzymes. Our data demonstrate for the first time that biotin supplementation affects liver morphology in normal mice, and that these modifications are not paralleled with damage markers.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

“…Interestingly, biotin supplementation induced an increase in islet size and modified its typical architecture of alpha-cells at the periphery and beta-cells at the core [15]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

Riverón-Negrete

Sicilia-Argumedo

Álvarez-Delgado

et al. 2016

BioMed Research International

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“…The increasing academic and industrial interest in commercially viable synthetic strategies for its preparation [1][2][3][4] is justified by the continued growth of the global market demand and its potential therapeutic applications as an anti-diabetic drug, 5 Most of the literature on the synthesis of (C)-biotin emphasizes the importance of (3aS,6aR)-lactone 4 as a chiral intermediate in the industrial preparation of (C)-biotin. Its generation via the asymmetric de-symmetrization of meso-anhydrides such as 2 has been reported.…”

Section: Oppi Briefsmentioning

confidence: 99%

An Improved Large-scale Preparation of Roche Lactone, an Intermediate for the Synthesis of (+)-Biotin

Xiong

Shen

et al. 2015

Organic Preparations and Procedures International

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Effect of biotin supplementation on fatty acid metabolic pathways in 3T3‐L1 adipocytes

2018

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Several studies have shown that pharmacological concentrations of biotin decrease serum lipid concentrations and the expression of lipogenic genes. Previous studies on epididymal adipose tissue in mice revealed that 8 weeks of dietary biotin supplementation increased the protein abundance of the active form of AMPK and the inactive forms acetyl CoA carboxylase (ACC)‐1 and − 2, and decreased serum free fatty acid concentrations but did not affect lipolysis. These data suggest that pharmacological concentrations of the vitamin might affect fatty acid metabolism. In this work, we investigated the effects of pharmacological biotin concentrations on fatty acid synthesis, oxidation, and uptake in 3T3‐L1 adipocytes. Similar to observations in mice, biotin‐supplemented 3T3‐L1 adipose cells increased the protein abundance of active T172‐AMPK and inactive ACC‐1 and ‐2 forms. No changes were observed in the expression of the transcriptional factor PPARα and carnitine‐palmitoyltransferase‐1 (CPT‐1). Radiolabeled assays indicated a decrease in fatty acid synthesis; an increase in fatty acid oxidation and fatty acid incorporation rate into the lipid fraction between control cells and biotin‐supplemented cells. The data revealed an increase in the mRNA abundance of the fatty acid transport proteins Fatp1 and Acsl1 but not Cd36 or Fatp4 mRNA. Furthermore, the abundance of glycerol phosphate acyl transferase‐3 protein was increased. Triglyceride content was not affected. Lipid droplet numbers showed an increase and their areas were smaller in the biotin‐supplemented group. In conclusion, these data indicate that biotin supplementation causes a decrease in fatty acid synthesis and an increase in its oxidation and uptake. © 2018 BioFactors, 45(2):259–270, 2019

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Effects of biotin supplementation in the diet on insulin secretion, islet gene expression, glucose homeostasis and beta-cell proportion

Cited by 58 publications

References 58 publications

Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

An Improved Large-scale Preparation of Roche Lactone, an Intermediate for the Synthesis of (+)-Biotin

Effect of biotin supplementation on fatty acid metabolic pathways in 3T3‐L1 adipocytes

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