Effects of Black Pepper (Piper nigrum L.) Chloroform Extract on the Enzymatic Activity and Metabolism of Escherichia coli and Staphylococcus aureus

Chen, Wenxue; Zou, Li; Hu, Yueying; Chen, Haiming

doi:10.1155/2018/9635184

Cited by 10 publications

(6 citation statements)

References 33 publications

(34 reference statements)

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“…In evaluating antimicrobial and antifungal activities of leaf extracts and essential oils extracted from black pepper (P. nigrum L.), significant action has been found against different microorganisms, namely, Pseudomonas aeruginosa, and Candida albicans, Staphylococcus aureus, Salmonella typhi, and Escherichia coli [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Activity and Cytotoxicity Effects of Extracts of Piper nigrum L. and Piperine

et al. 2022

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P. nigrum L. extracts and the piperine alkaloid have important antimicrobial, anti-inflammatory, and antioxidant properties. Therefore, in this study, we evaluated the antimicrobial activity and cytotoxicity of P. nigrum L. extracts and piperine, a compound isolated from the extracts of P. nigrum L. Extracts obtained via maceration, soxhlet, and purification steps, in addition to isolated piperine, were used in this study. Spectroscopic methods, such as nuclear magnetic resonance, scanning electron microscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry, were used to characterize piperine. In the microbiological analyses, the extract obtained via maceration-derived sample showed high efficiency in inhibiting Salmonella spp. (MIC < 100 μg/mL). The extract obtained via a soxhlet-derived sample showed promising inhibitory activity against almost all microorganisms, with negligible inhibition of Pseudomonas aeruginosa. Favorable inhibition coefficients were also observed against Staphylococcus aureus and Salmonella spp. (MIC < 100 μg/mL) for the extract obtained via purification of the steps-derived sample. Piperine showed an excellent inhibition coefficient against most microorganisms, with inactivity only observed against P. aeruginosa. Cytotoxicity evaluation assays in cancer cell lines revealed that piperine exhibited inhibitory potential on all tested tumor cell lines, causing a decrease in cell viability and achieving an IC50 of less than 30 μg/mL. The analyzed extracts from P. nigrum L. seeds showed cytotoxic activity against tumor and non-tumor cell lines.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Activity and Cytotoxicity Effects of Extracts of Piper nigrum L. and Piperine

et al. 2022

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“…The area (%) of 1H-Cycloprop[e]azulen-7-ol in the stem is higher than in the leaf samples. It is also found in black pepper (Piper ningrum L.), which has antibacterial activities against Escherichia coli and S. aureus (Chen W et al 2018). 1H-Cycloprop[e]azulen-7-ol found in Solanum melongena has pesticide activity (Umaru et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Phytochemical and gas chromatography-mass spectrometry profiling of two plant parts of Sandoricum koetjape

SAADAH¹,

TULANDI²,

ROHMAN³

2022

Biodiversitas

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Abstract. Saadah S, Tulandi SM, Rohman RA. 2022. Phytochemical and gas chromatography-mass spectrometry profiling of two plant parts of Sandoricum koetjape. Biodiversitas 23: 6199-6207. Sandoricum koetjape has been used in traditional Indonesian medicine for centuries. In Indonesia, the stem and leaves were used to treat helminthiasis, cough, stomachache, diarrhea, bloating, leucorrhoea, colic, and fever. To ascertain the phytochemical constituents of each plant part, and provide a sufficient basis for clinical application, so Gas Chromatography-Mass Spectrometry (GC/MS) analysis of stems and leaves was established. Phytochemical screening showed that stems and leaves contained alkaloids, flavonoids, quinone, triterpenoids, and tannin. Total phenolics in the leaves are higher than in the stem. Saponin and steroids were not detected. The GC/MS analysis indicated that the leaf extract contained more identified compounds (30) than the stem extract (28). Both S. koetjape samples contained 1H-Cycloprop[e]azulen-7-ol. The major compounds of the stem extract of S. koetjape are Fonenol (12.89%), 1H-Cyclopenta[1,3]cyclopropa[1,2]benzene (12.54%), and 1H-Cycloprop[e]azulen-7-ol (11.70%), while the major compounds in leaf methanol extract were 14,15-didehydro-Cyclodecacyclotetradecene (23.71%), 1H-Cycloprop[e]azulen-7-ol (17.04%), and Solanesol (8.34%). The two main components of the variable can account for 50.92% of the total variance, according to Principal Component Analysis (PCA) (PC1 is 21.19% and PC2 is 29.74%). These results indicate that the two components effectively categorize samples with distinct characteristics. The groups were divided based on the type of sample used, namely stem and leaf samples. According to the loading plot, 1H Cyclopenta[1,3]cyclopropa[1,2]benzene and Fonenol are two components that are very effective in categorizing samples based on their type. Both metabolites are higher in the stem than in the leaf. However, the metabolites activities of S. koetjape stem and leaves extracts could be revealed by further studies using in silico and in vitro approaches.

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“…7. beta., 7a.beta.7balpha) reported to have antibacterial activity against bacteria Escherichia coli and S. aureus [53] . Based on Duke's phytochemical and ethnobotanical database 4-((1E)-3-Hydroxy-1-propenyl)-2methoxyphenol a phenolic compound reported to possess antimicrobial, antioxidant anti-inflammatory and analgesic [54] .…”

Section: Discussionmentioning

confidence: 99%

In silico molecular docking study of plant-based compounds from medicinal plant Lantana camara L. against Aedes aegypti L. protein

Anita¹,

Selvaraj²

2022

Int. J. Mosq. Res.

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In the present study, the ethanolic leaf extract of Lantana camara L. was analyzed for phytocomponents using GC-MS technique and in silico molecular docking studies of phytocompounds against sterol carrier protein-2 (1PZ4) of Aedes aegypti L. larvae was performed to identify the phytocompounds with larvicidal potential. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of the ethanolic leaf extract of L. camara L. revealed the occurrence of 30 phytocompounds that are known to possess a variety of therapeutic properties. Subsequently, ten compounds were selected for the molecular docking studies, of which the phytocompounds Pectolinaringenin, Naphthalene, decahydro-2, 2-dimethyl and Gamolenic acid were predicted to have potential larvicidal property. To the best of our knowledge, this is the first study to predict the larvicidal activity of Pectolinaringenin, Naphthalene, decahydro-2, 2dimethyl and Gamolenic from L. camara against Ae. Aegypti.

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Effects of Black Pepper (Piper nigrum L.) Chloroform Extract on the Enzymatic Activity and Metabolism of Escherichia coli and Staphylococcus aureus

Cited by 10 publications

References 33 publications

Evaluation of Antimicrobial Activity and Cytotoxicity Effects of Extracts of Piper nigrum L. and Piperine

Evaluation of Antimicrobial Activity and Cytotoxicity Effects of Extracts of Piper nigrum L. and Piperine

Phytochemical and gas chromatography-mass spectrometry profiling of two plant parts of Sandoricum koetjape

In silico molecular docking study of plant-based compounds from medicinal plant Lantana camara L. against Aedes aegypti L. protein

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