Effects of blood anticoagulants on stable isotope values of sea turtle blood tissue

Lemons, Garrett E.; Eguchi, Tomoharu; Lyon, Boyd N.; LeRoux, Robin A.; Seminoff, Jeffrey A.

doi:10.3354/ab00397

Cited by 44 publications

(42 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Whole blood and the cellular component have very similar stable isotopic ratios (Cherel et al, 2005), allowing direct comparison. Although, EDTA may affect the stable isotopic ratios of blood (Lemons et al, 2012), EDTA tends to stay in the plasma when blood is centrifuged, and thus the effect on the cellular component should be negligible (Käkelä et al, 2007). Humpback whale skin was sampled at sea nearby the capelin pre-spawning staging area (Greenspond Island; n = 18) and spawning sites (Gull Island; n = 12; Figure 1).…”

Section: Study Site and Field Methodsmentioning

confidence: 99%

Dietary Niche Shifts of Multiple Marine Predators under Varying Prey Availability on the Northeast Newfoundland Coast

et al. 2017

View full text Add to dashboard Cite

Understanding species interactions among top marine predators and interactions with their prey can provide important insight into community-level responses to changing prey availability and the role of apex predators as indicators of ecosystem change. On the northeast Newfoundland coast, marine predators rely on capelin (Mallotus villosus), a dominant forage fish, as a food source. Capelin migrate into coastal regions to spawn during July, essentially transforming the food supply from low during early summer (i.e., pre-spawning) to high later in the summer (i.e., spawning). During July-August, 2016, we used stable isotopic ratios of nitrogen (δ 15 N) and carbon (δ 13 C) to investigate shifts in dietary niche metrics at the predator group-level (trophic position, dietary niche breadth) and community-level (niche overlap, trophic diversity) for multiple marine predators under varying capelin availability. Predator groups included non-breeding shearwaters (great shearwater Ardenna gravis, sooty shearwater A. grisea), humpback whales (Megaptera novaeangliae), and gull chicks (herring gull Larus argentatus, great black-backed gull Larus marinus). We also tested the sensitivity of community-level dietary metrics to a variety of published trophic discrimination factors. Tissue samples from shearwaters (blood cellular component), gull chicks (whole blood), and whales (skin), representing average diets over 2-3 weeks, were collected during three periods (early, mid, late) corresponding to increasing capelin availability. Isotopic niche breadth (Standard Ellipse Area, SEA b ) narrowed and trophic position shifted toward higher δ 15 N for all predator groups as capelin availability increased, suggesting a higher reliance on capelin. Trophic diversity (distance to centroid) decreased with increased capelin availability, while pairwise niche overlap between predator groups was highly variable and sensitive to trophic discrimination factors. Findings suggest that although capelin is the dominant forage fish during the summer, predators rely on capelin as prey to varying degrees. Combining species-and community-level metrics of dietary niche and trophic diversity may provide a more complete picture of predator responses to prey availability and, thus, may be important monitoring tools to indicate changes in the food supply of marine predators.

show abstract

Section: Study Site and Field Methodsmentioning

confidence: 99%

Dietary Niche Shifts of Multiple Marine Predators under Varying Prey Availability on the Northeast Newfoundland Coast

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Individuals were selected from a random sample of 200 nests distributed throughout the colony that were marked at the start of the breeding season. Samples were taken from the brachial vein (non-heparinised syringe and 23-gauge needle, Microlance; Samour et al 1983) (Lemons et al 2012). All samples were frozen and stored at −20°C until prepared for analysis.…”

Section: Stable Isotope Samplesmentioning

confidence: 99%

Drivers of intrapopulation variation in resource use in a generalist predator, the macaroni penguin

Horswill¹,

Matthiopoulos²,

Ratcliffe³

et al. 2016

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

“…Addition of heparin does not affect the carbon and nitrogen stable isotope composition of plasma and red blood cell samples (Lemons et al . ). Protein is the primary organic solute found in blood, and SIA of this tissue can indicate the dietary source of protein (but see Podlesak & McWilliams ).…”

Section: Methodsmentioning

confidence: 97%

Tracking Nutrient Routing in Avian Consumers in a Subtropical Desert

Osorio

2016

Biotropica

View full text Add to dashboard Cite

The nutrients animals ingest are allocated to serve different functions. We used contrasting C stable isotope signatures of dominant vegetation types in a North American subtropical desert to decipher how avian consumers allocate nutrients to fuel oxidative metabolism and to construct tissues. We conducted C stable isotope analysis of breath and feathers collected from nectarivores (hummingbirds) and of breath, plasma, and red blood cell samples collected from frugivores, granivores, and insectivores. Based on varying nutrient characteristics of food sources, we expected that for frugivores and granivores, CAM-derived food (RC CAM ) would have similar importance for oxidative metabolism and for tissue building, that RC CAM in nectarivores and insectivores would be more important for fueling metabolism than for generating tissues, and that (although low) RC CAM in insectivores would be higher for sustaining metabolism than for building tissues. Our predictions held true for nectarivores and granivores, but RC CAM use in tissue building was lower than expected in frugivores and higher than expected in insectivores. Our examination at the trophic guild, population, and individual levels showed that in general, nutrients used to sustain oxidative metabolism and tissue construction had a uniform isotopic origin. This finding suggests that the avian community under investigation does not route different food groups to fulfill different needs. However, we found some exceptions, indicating that birds can use different food sources for different functions, irrespective of trophic guild.Abstract in Spanish is available with online material.

show abstract

Effects of blood anticoagulants on stable isotope values of sea turtle blood tissue

Cited by 44 publications

References 30 publications

Dietary Niche Shifts of Multiple Marine Predators under Varying Prey Availability on the Northeast Newfoundland Coast

Dietary Niche Shifts of Multiple Marine Predators under Varying Prey Availability on the Northeast Newfoundland Coast

Drivers of intrapopulation variation in resource use in a generalist predator, the macaroni penguin

Tracking Nutrient Routing in Avian Consumers in a Subtropical Desert

Contact Info

Product

Resources

About