Effects of boron derivatives on extracellular matrix formation

Benderdour, Mohamed; Bui, T. V.; Heß, K.; Dıcko, Amadou; Belleville, F.; Dousset, B.

doi:10.1016/s0946-672x(00)80006-1

Cited by 61 publications

(32 citation statements)

References 17 publications

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“…They investigated the direct effect of B on specific enzymes (elastase, trypsin-like enzymes, collagenase, and alkaline phosphatase) implicated in extracellular matrix turnover, and they found that B may regulate these enzymes in fibroblasts. Benderdour et al [31] also discovered that boric acid solution improved wound healing through action on the extracellular matrix. By ALP staining, we discovered that BMSCs treated with 10 and 100 ng/ml boric acid presented a higher ALP activity compared with control.…”

Section: Discussionmentioning

confidence: 99%

Effect of Boron on Osteogenic Differentiation of Human Bone Marrow Stromal Cells

Ying

Cheng

Wang

et al. 2011

Biol Trace Elem Res

123

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Bone marrow stromal cells (BMSCs) have been well established as an ideal source of cell-based therapy for bone tissue engineering applications. Boron (B) is a notable trace element in humans; so far, the effects of boron on the osteogenic differentiation of BMSCs have not been reported. The aim of this study was to evaluate the effects of boron (0, 1, 10,100, and 1,000 ng/ml) on osteogenic differentiation of human BMSCs. In this study, BMSCs proliferation was analyzed by cell counting kit-8 (CCK8) assay, and cell osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity assay, Von Kossa staining, and real-time PCR. The results indicated that the proliferation of BMSCs was no different from the control group when added with B at the concentration of 1, 10, and 100 ng/ml respectively (P > 0.05); in contrast, 1,000 ng/ml B inhibited the proliferation of BMSCs at days 4, 7, and 14 (P < 0.05). By ALP staining, we discovered that BMSCs treated with 10 and 100 ng/ml B presented a higher ALP activity compared with control (P < 0.05). By real-time PCR, we detected the messenger RNA expression of ALP, osteocalcin, collagen type I, and bone morphogenetic proteins 7 were also increased in 10 and 100 ng/ml B treatment groups (P < 0.05). The calcium depositions were increased in 1 and 10 ng/ml B treatment groups (P < 0.05). Taken all together, it was the first time to report that B could increase osteogenic effect by stimulating osteogenic differentiation-related marker gene synthesis during the proliferation and differentiation phase in human BMSCs and could be a promising approach for enhancing osteogenic capacity of cell-based construction in bone tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Effect of Boron on Osteogenic Differentiation of Human Bone Marrow Stromal Cells

Ying

Cheng

Wang

et al. 2011

Biol Trace Elem Res

123

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“…In animal studies, boron has a more pronounced beneficial effect on bone[172728] and mineral metabolism[92930] than on soft tissues. [3132] This may help explain the ineffectiveness of boron in healing mucositis.…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary boron on 5-fluorouracil induced oral mucositis in rats

et al. 2013

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Objective:The aim of this study was to evaluate the effect of boron on 5-fluorouracil (5-FU)–induced oral mucositis in rats.Materials and Methods:Sixty-four male Wistar albino rats were injected with 5-FU on days 1 and 3. The right cheek pouch mucosa was scratched with the tip of an 18-G needle, dragged twice in a linear movement, on days 3 and 5. The animals were randomly divided into two groups of 32: boron group (BG) and control group (CG). Rats in the CG did not receive any treatment, whereas the others were fed boron (3 mg·kg-1·day-1) by gavage. The animals were sacrificed on day 3 (n = 8), 6 (n = 8), 9 (n = 8), and 12 (n = 8), and the cheek pouch was removed for histopathological analysis.Results:On day 3, both groups showed necrosis and active inflammation, but the inflammation was mild in CG and moderate in BG. On day 6, both BG and CG showed necrosis; in the CG, there was moderate inflammation, and in the BG, there was severe inflammation and granulation tissue around the necrotic area. On day 9, re-epithelization began in both groups, and there were no differences between groups. Re-epithelization was complete in both groups on day 12.Conclusion:We found no beneficial effect of boron in healing oral mucositis. Additional research is warranted to elucidate the pathogenic inflammatory mechanisms involved in mucositis and the prophylactic and therapeutic roles of antioxidants.

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“…They investigated the direct effect of B on specific enzymes (elastase, trypsin-like enzymes, collagenase and alkaline phosphatase) implicated in extracellular matrix turnover and they found that B may regulate these enzymes in fibroblasts, in vitro [26]. Benderdour et al also established that boric acid solution improved wound healing through action on the extracellular matrix, in vitro [27]. In our study, we also found that B regulates mRNA expression of extracellular matrix proteins including COL I, OPN, BSP and OCN which are mineralized tissue-associated proteins.…”

Section: Discussionmentioning

confidence: 99%