Effects of Boron on Cytotoxicity, Apoptosis, and Cell Cycle of Cultured Rat Sertoli Cells In vitro

Lu, Liangyue; Zhang, Qian; Ren, Man; Jin, Erhui; Hu, Qianqian; Zhao, Chunfang; Li, Shenghe

doi:10.1007/s12011-019-01911-3

Cited by 15 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Xiaoting Zhang et al [14] reported that low concentrations (0.01-0.1 mmol/L) of boron promoted the proliferation and inhibited the apoptosis of ostrich spleen lymphocytes by enhancing cell membrane functions as well as intracellular catalase activities, whereas high concentrations (25-100 mmol/L) of boron exerted the opposite effects on cells. Their ndings are comparable with those of our previous studies [15,16] where we found that boron concentrations below 0.5 mmol/L signi cantly improved the viabilities of testicular Sertoli cells in vitro and inhibited their apoptosis. However, high doses of boron (concentrations above 5.0 mmol/L) exhibited signi cant toxic effects, inhibiting cell viabilities, accelerating Sertoli cell apoptosis, and arresting the cell cycle in the G0/G1 phase.…”

Section: Discussionsupporting

confidence: 92%

Effects of boron on fat synthesis in porcine mammary epithelial cells

Yang

Ren

2022

Preprint

Self Cite

View full text Add to dashboard Cite

This study aimed to investigate the effect of boron on porcine mammary epithelial cell (PMEC) survival, cell cycle and milk fat synthesis. PMECs from boron-treated groups were exposed to 0, 0.1, 0.2, 0.3, 0.4, 0.8, 1, 10, 20, 40, and 80 mmol/L boric acid concentrations. Cell Counting Kit-8 and flow cytometry assays were performed to assess cell survival and the cell cycle, respectively. Triacylglycerol (TAG) levels in PMECs and culture medium were determined by a Triacylglycerol kit while PMEC lipid droplet aggregation was investigated via Oil Red Staining. Milk fat synthesis-associated mRNA levels were determined by real-time fluorescence quantitative PCR while its protein expressions were determined by Western blot. Low (0.2, 0.3, 0.4 mmol/L) and high (>10 mmol/L) boron concentrations significantly promoted and inhibited cell viabilities, respectively. Boron (0.3 mmol/L) markedly elevated the abundance of G2/M phase cells. Ten mmol/L boron significantly increased the abundances of G0/G1 and S phase cells, but markedly suppressed G2/M phase cell abundance. At 0.3 mmol/L, boron significantly enhanced ERK phosphorylation while at 0.4, 0.8, 1, and 10 mmol/L, it markedly decreased lipid droplet diameters. Boron (10 mmol/L) significantly suppressed ACACA and SREBP1 protein expressions. The FASN protein levels were markedly suppressed by 0.4, 0.8, 1 and 10 mmol/L boron. Both 1 and 10 mmol/L markedly decreased FASN and SREBP1 mRNA expressions. Ten mmol/L boron significantly decreased PPARα mRNA levels. Low concentrations of boron promoted cell viability, while high concentrations inhibited PMEC viabilities and reduced lipid droplet diameters, which shows the implications of boron in pregnancy and lactation.

show abstract

Section: Discussionsupporting

confidence: 92%

Effects of boron on fat synthesis in porcine mammary epithelial cells

Yang

Ren

2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cell cycle results also supported cell viability and apoptosis analysis. Similar to these results, a study showed that low doses of boron application increase cell viability and G2 phase cell accumulation in primary rat cells [29]. It has been reported that a different natural product, propolis protected HaCaT cells against apoptosis induced by UV-irradiation [8,30].…”

Section: Discussionsupporting

confidence: 60%

The Protective Effects of Sodium Pentaborate Tetrahydrate Against UVB-induced Apoptosis in Human Keratinocytes

Abdik

2022

Hittite Journal of Science and Engineering

View full text Add to dashboard Cite

Ultraviolet radiation (UV) is an environmental carcinogen causing human skin cancer. Exposure of the skin to UV produces apoptotic keratinocytes called sunburn cells within the epidermis. Boron, an essential element for plants, has several biological properties, such as anti-cancer, anti-microbial, and anti-oxidant. In the present study, the possible protective effects of sodium pentaborate pentahydrate (SPT) against UVB-induced apoptosis in human keratinocyte cells, HaCaT, were investigated. They were treated with SPT at different concentrations (7.8-125 μg/mL) for 24h after UVB irradiation (20, 30 and 60mJ/cm2). Cell viability, annexin V assay, cell cycle analysis, and apoptosis-related gene levels were measured using RT-PCR. Treatment with SPT (15.6-31.25μg/mL) after 30 mJ/m2 UVB exposure significantly increased cell survival. Annexin V apoptosis analysis demonstrated a robust protective effect by treatment with SPT at concentrations of 15.6 and 31.25μg/mL after 30mJ/cm2 UVB irradiation. The cell cycle analysis revealed that UVB irradiation elevated the number of cells at the G0/G1 phase while SPT treatment after UVB irradiation increased the number of cells at G2/M phase, suggesting the changes were partially reversed. Furthermore, treatment with 15.6μg/mL SPT after 30 mJ/m2 UV irradiation blocked the activation of caspase 3, caspase 9, Bax, and p53. These results indicate that treatment with SPT exerts protective effects after UVB irradiation. Thus, treatment with SPT led to strong protection against UVB-induced apoptotic cell death in HaCaT cells.

show abstract

“…Similarly, in a study reveals that both synthesized hexagonal boron nitrides and boric acid are not cytotoxic at lower than 22 µg/mL boron containing concentrations on brain neuronal mHippo E-14 cells [42]. Administration of < 0.5 mmol/L boron significantly promoted the viability of Sertoli cells (P < 0.01); however, the exposure to high dose (> 10 mmol/L) of boron exhibited significant adverse effects [43]. Deshayes et al [44] observed cytotoxic effects on B16F10 murine melanoma cells of phenyl boronic acid nanoparticles (IC 50 :186 µM).…”

Section: Number Of Revertant Coloniesmentioning

confidence: 86%

A new approach for prevention the oxidations and mutations: zinc borate

Uğur¹,

Ceylan²,

Boran³

et al. 2019

Journal of Boron

View full text Add to dashboard Cite

Zinc borate is a white crystalline powder substance with variable composition (34% B 2 O 3 , 45% ZnO and 20% H 2 O). It is used as a fungus and mildew inhibitor, to fire proof textiles, and for other uses. Although there are limited studies about the biologic properties of this compound. The present study was aimed to investigate the antioxidant and antimutagenic activity of zinc borate and also to figure out its cytotoxic effect. Antioxidant activity of zinc borate was determined with βcarotene linoleic acid and DPPH radical scavenging assays. The mutagenicity and antimutagenic activity was determined with AMES/ Salmonella microsomal test systems using Salmonella typhimurium TA98 and TA100 strains. The cytotoxic effect of zinc borate was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using mouse fibroblast L929 cell line. When compared to the control, % inhibition values of 1 mg/mL zinc borate for DPPH radical scavenging and β-carotene linoleic acid tests were calculated as 13.50% and 30%, respectively. At the tested concentrations, zinc borate did not exhibit any mutagenic effect. Zinc borate exhibited low antimutagenic activity at 0.2 and 0.04 mg/plate concentrations on S. typhimurium TA98 strain while it exhibited no antimutagenic activity on S. typhimurium TA100 strain. In vitro toxicity against L929 cell line with IC 50 values of zinc borate were found to be 40.70 µg/mL and 32.93 µg/ mL for 24h and 72h, respectively. In spite of the low DPPH free radical scavenging activity, zinc borate has found to have moderate total antioxidant activity besides low antimutagenic potential. Zinc borate has a potential to use for medical purpose with its antioxidative and antimutagenic properties. 196

show abstract

Effects of Boron on Cytotoxicity, Apoptosis, and Cell Cycle of Cultured Rat Sertoli Cells In vitro

Cited by 15 publications

References 22 publications

Effects of boron on fat synthesis in porcine mammary epithelial cells

Effects of boron on fat synthesis in porcine mammary epithelial cells

The Protective Effects of Sodium Pentaborate Tetrahydrate Against UVB-induced Apoptosis in Human Keratinocytes

A new approach for prevention the oxidations and mutations: zinc borate

Contact Info

Product

Resources

About