Effects of Bothrops moojeni venom on Leishmania amazonensis promastigote forms

Castilhos, Patrícia de; Pereira, Camila G.; Silva, Aln; Napolitano,; Oliveira, Fábio de; Souza, Mariana Assunção de

doi:10.1590/s1678-91992011000200005

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…Studies involving Leishmania spp. have focused on the extracellular form of the parasite, known as promastigote, instead of the amastigote form, due to easier in vitro growth and not requiring another cell culture such as macrophages (Castilhos et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Phenolic composition and antiparasitic activity of plants from the Brazilian Northeast “Cerrado”

Júnior

Morais

Gómez

et al. 2016

Saudi Journal of Biological Sciences

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This work describes the antiparasitic and cytotoxic activities of three plant species from the Cerrado biome, Northeastern Brazil. Significant antiparasitic inhibition was observed against Trypanosoma cruzi (63.86%), Leishmania brasiliensis (92.20%) and Leishmania infantum (95.23%) when using ethanol extract from leaves of Guazuma ulmifolia Lam. (Malvaceae), at a concentration of 500 μg/mL. However, low levels of inhibition were observed when assessing leishmanicidal and trypanocidal (Clone CL-B5) activities of crude ethanol extracts from leaves and bast tissue of Luehea paniculata (Malvaceae) and leaves and bark of Prockia crucis (Salicaceae) at a concentration of 500 μg/mL. The extracts revealed the presence of phenolic acids such as gallic acid, chlorogenic acid, caffeic acid and rosmarinic acid, as well as flavonoids such as rutin, luteolin, apigenin and quercetin - the latter detected only in G. ulmifolia. G. ulmifolia extract displayed higher leishmanicidal activity probably due to the presence of quercetin, a potent known leishmanicidal compound. A cytotoxicity test indicated values over 50% at the highest concentration (1000 μg/mL) for all natural products, which were considered cytotoxic. This points out the need for further tests to enable future in vivo trials, including antineoplastic activity on human tumor cells.

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Section: Resultsmentioning

confidence: 99%

Phenolic composition and antiparasitic activity of plants from the Brazilian Northeast “Cerrado”

Júnior

Morais

Gómez

et al. 2016

Saudi Journal of Biological Sciences

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“…The only drug available in Brazil within the highly limited therapeutic arsenal is benznidazole, which presents limited efficacy in the chronic phase and high toxicity [ 5 ]. Thus, there is an essential need for the discovery of new drug entities, using animal toxins as a source [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Antileishmanial and antitrypanosomal activity of the cutaneous secretion of Siphonops annulatus

Pinto

Antoniazzi

Jared

et al. 2014

J Venom Anim Toxins incl Trop Dis

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BackgroundAmong the tropical parasitic diseases, those caused by protozoans are considered a challenge to public health, being represented by leishmaniasis and Chagas disease. In view of the low effectiveness and toxicity of the current therapy, animal venoms such as amphibian secretions have been used as a promising source of new drug prototypes. The present work aimed to achieve bioguided fractionation of metabolites present in a cutaneous secretion of the caecilian Siphonops annulatus (Amphibia: Gymnophiona: Siphonopidae) with antileishmanial and antitrypanosomal activity.MethodsThrough liquid-liquid partition and chromatographic techniques, the secretion was fractionated using bioguided assays. The 50% inhibitory concentration (IC50) of the main fraction (SaFr1) was studied against Leishmania (L.) infantum promastigotes and intracellular amastigotes, trypomastigotes of Trypanosoma cruzi and mammalian cells; viability was detected by the colorimetric MTT assay. By using a spectrofluorimetric assay with the probe SYTOX® Green and transmission electron microscopy (TEM), we also investigated the potential damage caused by SaFr1 in the plasma membrane and mitochondria of Leishmania.ResultsThe bioguided assay enabled isolation of a highly purified fraction (SaFr1) with an IC50 of 0.065 μg/mL against promastigotes and 2.75 μg/mL against trypomastigotes. Due to its high toxicity to peritoneal macrophages, SaFr1 showed no selectivity towards the intracellular forms of Leishmania. Ultrastructural studies with Leishmania demonstrated severe mitochondrial damage and the formation of large cytoplasmic vacuoles, leading to the parasite’s death within a few hours. Nevertheless, it caused no alteration in the plasma membrane permeability as detected by the fluorescent probe and TEM.ConclusionsThe present study demonstrated for the first time the antiparasitic activity of the skin secretion of the caecilian S. annulatus against Leishmania and T. cruzi, confirming that skin secretions of these amphibians, similarly to those of anurans and salamanders, are also potential tools for the development of new drug candidates against neglected diseases.

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Synergism of in vitro plasmodicidal activity of phospholipase A2 isoforms isolated from panamanian Bothrops asper venom

Simões-Silva

Alfonso

Gomez

et al. 2021

Chemico-Biological Interactions

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Effects of Bothrops moojeni venom on Leishmania amazonensis promastigote forms

Cited by 5 publications

References 29 publications

Phenolic composition and antiparasitic activity of plants from the Brazilian Northeast “Cerrado”

Phenolic composition and antiparasitic activity of plants from the Brazilian Northeast “Cerrado”

Antileishmanial and antitrypanosomal activity of the cutaneous secretion of Siphonops annulatus

Synergism of in vitro plasmodicidal activity of phospholipase A2 isoforms isolated from panamanian Bothrops asper venom

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