Effects of bound phenolic from defatted Moringa oleifera seed flour on diet-induced hypercholesterolemic mice

Abstract

The effect of bound phenolic and catechins extracted from defatted Moringa oleifera seed (DMSF) flour on diet‐induced hypercholesterolemia was evaluated in this study. The animals (C57BL6 mice) were divided into four groups: group one received AIN 93M diet (control) group two received high‐fat cholesterol diet (HFCD), three and four received HFCD along with bound phenolic and catechins, respectively, through oral administration at the dose of 25 mg/kg body weight daily for 8 weeks. Blood and tissue analysis showed that bound phenolic and catechins were able to reduce plasma and liver cholesterol significantly (p < .05). And these extracts were also able to reduce stress enzymes and molecules level in liver. The bound phenolic and catechins extracts from DMSF were able to increase plasma HDL level and lower atherogenic index in the treated groups. The data suggests that the combination of HFCD and bound phenolic or catechins extracted from DMSF as a source of antioxidants was beneficial. Practical applications In the present study the bound phenolic and catechins extracted from DMSF was studied for its effect on reducing cholesterol. DMSF is a processing waste left over after extraction of oil, hence prove to be cost‐effective. In this study catechins (catechin and epicatechin) as well as combination of catechin‐rich bound phenolic containing gallic acid, protocatechuic acid, ferulic acid, and catechin gallate were studied for its hypocholesterolemic effect. The results revealed that the combination of bound phenolic or catechins were comparably beneficial in reducing cholesterol and atherogenic index in diet induced mice model, which is the critical risk factor for atherosclerosis.

Thiamethoxam-induced hematological, biochemical, and genetic alterations and the ameliorated effect of Moringa oleifera in male mice

“…Moringa oleifera is widely utilized in alternative medicine for its antioxidant and health-promoting characteristics [ 60 ]. Its leaves are a good source of bioactive and phenolic substances, which have been shown to protect against chronic illnesses associated with oxidative damage [ 61 ].…”

Antioxidant traits and protective impact ofMoringa oleiferaleaf extract against diclofenac sodium-induced liver toxicity in rats

“…Moringa oleifera is commonly applied in folk medicine via its antioxidant and healt‐promoting properties. Yet, its biological activities are not limited to the antioxidant capacity (Pandu, Cherupanalli, & Muthukumar, ). M. oleifera leaves are a great source of bioactives and phenol compounds, such as flavonoids and phenolic acids.…”

Moringa oleifera defatted flour: Nutritive and bioactive impact of shells

“…We have further detailed the main phenolic compounds by HPLC after alkaline hydrolysis (Govardhan‐Singh, Negi, & Radha, 2013), with catechin as a main phenolic compound, in agreement with the composition given by the previous authors for kernel flour. Again, the results for whole seed flour were higher than those obtained for the kernel flour (7.15 vs. 3.40 mg/kg, respectively), indicating that shells are an important source of these compounds, whose positive effect in the lipid profile of diet‐induced hypercholesterolemic mice has been reported (Pandu, Cherupanalli, & Muthukumar, 2018). Our results are within the expected amounts based on Ijarotimi et al (2013) findings, but lower than the ones reported by Govardhan‐Singh et al.…”