Effects of bovine serum albumin during preservation of boar semen at 17 °C

Abstract

Bovine serum albumin (BSA) has been reported to improve sperm quality, primarily by enhancing sperm motility, viability, membrane integrity and acrosome integrity. In this study, crossbred (75% LWY x 25% Zovawk) boars' semen samples were collected and diluted with GEPS extender containing different concentration (control, 5, 10 and 15%) of BSA at 24 hours of preservation and recorded for sperm motility, per cent live sperm, membrane integrity and acrosome integrity. The results showed that supplementation of BSA showed significantly (P<0.01) higher in sperm parameters compared to control. Interestingly we have found that 10% BSA level group was significantly (P<0.01) higher sperm motility, per cent live sperm, membrane integrity and acrosome integrity which was followed by 5% BSA, 15% BSA and control group at 24 hours of preservation at 17°C. Taken together these results suggested that BSA had a positive role in the regulation of crossbred (75% LWY x 25% Zovawk) boar sperm quality.